[2023-03-15 10:21:47,997] [INFO] DFAST_QC pipeline started.
[2023-03-15 10:21:47,998] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 10:21:47,998] [INFO] DQC Reference Directory: /var/lib/cwl/stgb03938c4-3459-432c-9663-fe877828643b/dqc_reference
[2023-03-15 10:21:49,088] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 10:21:49,088] [INFO] Task started: Prodigal
[2023-03-15 10:21:49,088] [INFO] Running command: cat /var/lib/cwl/stg1aedb3e1-5733-435b-a8d0-5d6f872bc029/OceanDNA-a1593.fa | prodigal -d OceanDNA-a1593/cds.fna -a OceanDNA-a1593/protein.faa -g 11 -q > /dev/null
[2023-03-15 10:21:51,403] [INFO] Task succeeded: Prodigal
[2023-03-15 10:21:51,403] [INFO] Task started: HMMsearch
[2023-03-15 10:21:51,404] [INFO] Running command: hmmsearch --tblout OceanDNA-a1593/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb03938c4-3459-432c-9663-fe877828643b/dqc_reference/reference_markers.hmm OceanDNA-a1593/protein.faa > /dev/null
[2023-03-15 10:21:51,561] [INFO] Task succeeded: HMMsearch
[2023-03-15 10:21:51,562] [WARNING] Found 2/6 markers. [/var/lib/cwl/stg1aedb3e1-5733-435b-a8d0-5d6f872bc029/OceanDNA-a1593.fa]
[2023-03-15 10:21:51,566] [INFO] Query marker FASTA was written to OceanDNA-a1593/markers.fasta
[2023-03-15 10:21:51,566] [INFO] Task started: Blastn
[2023-03-15 10:21:51,566] [INFO] Running command: blastn -query OceanDNA-a1593/markers.fasta -db /var/lib/cwl/stgb03938c4-3459-432c-9663-fe877828643b/dqc_reference/reference_markers.fasta -out OceanDNA-a1593/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 10:21:53,771] [INFO] Task succeeded: Blastn
[2023-03-15 10:21:53,772] [INFO] Selected 2 target genomes.
[2023-03-15 10:21:53,772] [INFO] Target genome list was writen to OceanDNA-a1593/target_genomes.txt
[2023-03-15 10:21:53,774] [INFO] Task started: fastANI
[2023-03-15 10:21:53,774] [INFO] Running command: fastANI --query /var/lib/cwl/stg1aedb3e1-5733-435b-a8d0-5d6f872bc029/OceanDNA-a1593.fa --refList OceanDNA-a1593/target_genomes.txt --output OceanDNA-a1593/fastani_result.tsv --threads 1
[2023-03-15 10:21:54,553] [INFO] Task succeeded: fastANI
[2023-03-15 10:21:54,554] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb03938c4-3459-432c-9663-fe877828643b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 10:21:54,554] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb03938c4-3459-432c-9663-fe877828643b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 10:21:54,554] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 10:21:54,554] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 10:21:54,554] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 10:21:54,554] [INFO] DFAST Taxonomy check result was written to OceanDNA-a1593/tc_result.tsv
[2023-03-15 10:21:54,555] [INFO] ===== Taxonomy check completed =====
[2023-03-15 10:21:54,555] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 10:21:54,555] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb03938c4-3459-432c-9663-fe877828643b/dqc_reference/checkm_data
[2023-03-15 10:21:54,557] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 10:21:54,559] [INFO] Task started: CheckM
[2023-03-15 10:21:54,559] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a1593/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a1593/checkm_input OceanDNA-a1593/checkm_result
[2023-03-15 10:22:14,726] [INFO] Task succeeded: CheckM
[2023-03-15 10:22:14,726] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 63.38%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 10:22:14,727] [INFO] ===== Completeness check finished =====
[2023-03-15 10:22:14,727] [INFO] ===== Start GTDB Search =====
[2023-03-15 10:22:14,728] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a1593/markers.fasta)
[2023-03-15 10:22:14,731] [INFO] Task started: Blastn
[2023-03-15 10:22:14,731] [INFO] Running command: blastn -query OceanDNA-a1593/markers.fasta -db /var/lib/cwl/stgb03938c4-3459-432c-9663-fe877828643b/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a1593/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 10:22:16,364] [INFO] Task succeeded: Blastn
[2023-03-15 10:22:16,365] [INFO] Selected 6 target genomes.
[2023-03-15 10:22:16,365] [INFO] Target genome list was writen to OceanDNA-a1593/target_genomes_gtdb.txt
[2023-03-15 10:22:16,576] [INFO] Task started: fastANI
[2023-03-15 10:22:16,576] [INFO] Running command: fastANI --query /var/lib/cwl/stg1aedb3e1-5733-435b-a8d0-5d6f872bc029/OceanDNA-a1593.fa --refList OceanDNA-a1593/target_genomes_gtdb.txt --output OceanDNA-a1593/fastani_result_gtdb.tsv --threads 1
[2023-03-15 10:22:17,617] [INFO] Task succeeded: fastANI
[2023-03-15 10:22:17,622] [INFO] Found 6 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 10:22:17,622] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002718915.1	s__CG-Epi1 sp002718915	81.0009	88	146	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__MGIII;f__CG-Epi1;g__CG-Epi1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002497685.1	s__CG-Epi1 sp002497685	80.432	73	146	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__MGIII;f__CG-Epi1;g__CG-Epi1	95.0	99.79	99.79	0.70	0.70	2	-
GCA_016845825.1	s__CG-Epi1 sp016845825	79.8949	92	146	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__MGIII;f__CG-Epi1;g__CG-Epi1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_001875345.1	s__CG-Epi1 sp001875345	79.8533	97	146	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__MGIII;f__CG-Epi1;g__CG-Epi1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002724775.1	s__CG-Epi1 sp002724775	79.7033	84	146	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__MGIII;f__CG-Epi1;g__CG-Epi1	95.0	96.65	95.92	0.84	0.84	3	-
GCA_001875365.1	s__CG-Epi1 sp001875365	79.2832	68	146	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__MGIII;f__CG-Epi1;g__CG-Epi1	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 10:22:17,622] [INFO] GTDB search result was written to OceanDNA-a1593/result_gtdb.tsv
[2023-03-15 10:22:17,622] [INFO] ===== GTDB Search completed =====
[2023-03-15 10:22:17,623] [INFO] DFAST_QC result json was written to OceanDNA-a1593/dqc_result.json
[2023-03-15 10:22:17,623] [INFO] DFAST_QC completed!
[2023-03-15 10:22:17,623] [INFO] Total running time: 0h0m30s