[2023-03-15 07:25:31,270] [INFO] DFAST_QC pipeline started.
[2023-03-15 07:25:31,278] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 07:25:31,278] [INFO] DQC Reference Directory: /var/lib/cwl/stg64dc0595-3aa8-4f7c-ae41-570503a8c45f/dqc_reference
[2023-03-15 07:25:32,916] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 07:25:32,917] [INFO] Task started: Prodigal
[2023-03-15 07:25:32,917] [INFO] Running command: cat /var/lib/cwl/stg0970f29d-3128-44e0-ae64-762d72ec8bed/OceanDNA-a1796.fa | prodigal -d OceanDNA-a1796/cds.fna -a OceanDNA-a1796/protein.faa -g 11 -q > /dev/null
[2023-03-15 07:25:37,892] [INFO] Task succeeded: Prodigal
[2023-03-15 07:25:37,893] [INFO] Task started: HMMsearch
[2023-03-15 07:25:37,893] [INFO] Running command: hmmsearch --tblout OceanDNA-a1796/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg64dc0595-3aa8-4f7c-ae41-570503a8c45f/dqc_reference/reference_markers.hmm OceanDNA-a1796/protein.faa > /dev/null
[2023-03-15 07:25:38,046] [INFO] Task succeeded: HMMsearch
[2023-03-15 07:25:38,047] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg0970f29d-3128-44e0-ae64-762d72ec8bed/OceanDNA-a1796.fa]
[2023-03-15 07:25:38,113] [INFO] Query marker FASTA was written to OceanDNA-a1796/markers.fasta
[2023-03-15 07:25:38,114] [INFO] Task started: Blastn
[2023-03-15 07:25:38,114] [INFO] Running command: blastn -query OceanDNA-a1796/markers.fasta -db /var/lib/cwl/stg64dc0595-3aa8-4f7c-ae41-570503a8c45f/dqc_reference/reference_markers.fasta -out OceanDNA-a1796/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 07:25:38,547] [INFO] Task succeeded: Blastn
[2023-03-15 07:25:38,575] [INFO] Selected 5 target genomes.
[2023-03-15 07:25:38,575] [INFO] Target genome list was writen to OceanDNA-a1796/target_genomes.txt
[2023-03-15 07:25:38,577] [INFO] Task started: fastANI
[2023-03-15 07:25:38,577] [INFO] Running command: fastANI --query /var/lib/cwl/stg0970f29d-3128-44e0-ae64-762d72ec8bed/OceanDNA-a1796.fa --refList OceanDNA-a1796/target_genomes.txt --output OceanDNA-a1796/fastani_result.tsv --threads 1
[2023-03-15 07:25:41,282] [INFO] Task succeeded: fastANI
[2023-03-15 07:25:41,283] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg64dc0595-3aa8-4f7c-ae41-570503a8c45f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 07:25:41,283] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg64dc0595-3aa8-4f7c-ae41-570503a8c45f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 07:25:41,283] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 07:25:41,283] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 07:25:41,283] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 07:25:41,292] [INFO] DFAST Taxonomy check result was written to OceanDNA-a1796/tc_result.tsv
[2023-03-15 07:25:41,307] [INFO] ===== Taxonomy check completed =====
[2023-03-15 07:25:41,307] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 07:25:41,307] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg64dc0595-3aa8-4f7c-ae41-570503a8c45f/dqc_reference/checkm_data
[2023-03-15 07:25:41,358] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 07:25:41,363] [INFO] Task started: CheckM
[2023-03-15 07:25:41,363] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a1796/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a1796/checkm_input OceanDNA-a1796/checkm_result
[2023-03-15 07:26:01,947] [INFO] Task succeeded: CheckM
[2023-03-15 07:26:01,948] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 07:26:02,006] [INFO] ===== Completeness check finished =====
[2023-03-15 07:26:02,006] [INFO] ===== Start GTDB Search =====
[2023-03-15 07:26:02,007] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a1796/markers.fasta)
[2023-03-15 07:26:02,007] [INFO] Task started: Blastn
[2023-03-15 07:26:02,007] [INFO] Running command: blastn -query OceanDNA-a1796/markers.fasta -db /var/lib/cwl/stg64dc0595-3aa8-4f7c-ae41-570503a8c45f/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a1796/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 07:26:02,438] [INFO] Task succeeded: Blastn
[2023-03-15 07:26:02,462] [INFO] Selected 9 target genomes.
[2023-03-15 07:26:02,462] [INFO] Target genome list was writen to OceanDNA-a1796/target_genomes_gtdb.txt
[2023-03-15 07:26:02,471] [INFO] Task started: fastANI
[2023-03-15 07:26:02,471] [INFO] Running command: fastANI --query /var/lib/cwl/stg0970f29d-3128-44e0-ae64-762d72ec8bed/OceanDNA-a1796.fa --refList OceanDNA-a1796/target_genomes_gtdb.txt --output OceanDNA-a1796/fastani_result_gtdb.tsv --threads 1
[2023-03-15 07:26:04,443] [INFO] Task succeeded: fastANI
[2023-03-15 07:26:04,446] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 07:26:04,446] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_001875425.1	s__UBA102 sp001875425	99.7525	253	309	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__MGIII;f__CG-Epi1;g__UBA102	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002509225.1	s__UBA102 sp002509225	89.0155	256	309	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__MGIII;f__CG-Epi1;g__UBA102	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 07:26:04,448] [INFO] GTDB search result was written to OceanDNA-a1796/result_gtdb.tsv
[2023-03-15 07:26:04,453] [INFO] ===== GTDB Search completed =====
[2023-03-15 07:26:04,457] [INFO] DFAST_QC result json was written to OceanDNA-a1796/dqc_result.json
[2023-03-15 07:26:04,457] [INFO] DFAST_QC completed!
[2023-03-15 07:26:04,457] [INFO] Total running time: 0h0m33s