[2023-03-15 17:24:01,580] [INFO] DFAST_QC pipeline started.
[2023-03-15 17:24:01,580] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 17:24:01,580] [INFO] DQC Reference Directory: /var/lib/cwl/stgcd3c2ff8-9f27-48c2-9cc3-add3b91d7e91/dqc_reference
[2023-03-15 17:24:02,833] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 17:24:02,833] [INFO] Task started: Prodigal
[2023-03-15 17:24:02,833] [INFO] Running command: cat /var/lib/cwl/stg2e5421f8-6d12-4b0a-baa7-ce0c2cc724ff/OceanDNA-a1857.fa | prodigal -d OceanDNA-a1857/cds.fna -a OceanDNA-a1857/protein.faa -g 11 -q > /dev/null
[2023-03-15 17:24:09,548] [INFO] Task succeeded: Prodigal
[2023-03-15 17:24:09,549] [INFO] Task started: HMMsearch
[2023-03-15 17:24:09,549] [INFO] Running command: hmmsearch --tblout OceanDNA-a1857/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgcd3c2ff8-9f27-48c2-9cc3-add3b91d7e91/dqc_reference/reference_markers.hmm OceanDNA-a1857/protein.faa > /dev/null
[2023-03-15 17:24:09,718] [INFO] Task succeeded: HMMsearch
[2023-03-15 17:24:09,718] [WARNING] Found 2/6 markers. [/var/lib/cwl/stg2e5421f8-6d12-4b0a-baa7-ce0c2cc724ff/OceanDNA-a1857.fa]
[2023-03-15 17:24:09,731] [INFO] Query marker FASTA was written to OceanDNA-a1857/markers.fasta
[2023-03-15 17:24:09,732] [INFO] Task started: Blastn
[2023-03-15 17:24:09,732] [INFO] Running command: blastn -query OceanDNA-a1857/markers.fasta -db /var/lib/cwl/stgcd3c2ff8-9f27-48c2-9cc3-add3b91d7e91/dqc_reference/reference_markers.fasta -out OceanDNA-a1857/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 17:24:10,168] [INFO] Task succeeded: Blastn
[2023-03-15 17:24:10,169] [INFO] Selected 1 target genomes.
[2023-03-15 17:24:10,169] [INFO] Target genome list was writen to OceanDNA-a1857/target_genomes.txt
[2023-03-15 17:24:10,170] [INFO] Task started: fastANI
[2023-03-15 17:24:10,170] [INFO] Running command: fastANI --query /var/lib/cwl/stg2e5421f8-6d12-4b0a-baa7-ce0c2cc724ff/OceanDNA-a1857.fa --refList OceanDNA-a1857/target_genomes.txt --output OceanDNA-a1857/fastani_result.tsv --threads 1
[2023-03-15 17:24:10,452] [INFO] Task succeeded: fastANI
[2023-03-15 17:24:10,453] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgcd3c2ff8-9f27-48c2-9cc3-add3b91d7e91/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 17:24:10,453] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgcd3c2ff8-9f27-48c2-9cc3-add3b91d7e91/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 17:24:10,453] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 17:24:10,453] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 17:24:10,453] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 17:24:10,453] [INFO] DFAST Taxonomy check result was written to OceanDNA-a1857/tc_result.tsv
[2023-03-15 17:24:10,453] [INFO] ===== Taxonomy check completed =====
[2023-03-15 17:24:10,454] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 17:24:10,454] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgcd3c2ff8-9f27-48c2-9cc3-add3b91d7e91/dqc_reference/checkm_data
[2023-03-15 17:24:10,456] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 17:24:10,459] [INFO] Task started: CheckM
[2023-03-15 17:24:10,459] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a1857/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a1857/checkm_input OceanDNA-a1857/checkm_result
[2023-03-15 17:24:31,743] [INFO] Task succeeded: CheckM
[2023-03-15 17:24:31,744] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 68.30%
Contamintation: 0.93%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-03-15 17:24:31,745] [INFO] ===== Completeness check finished =====
[2023-03-15 17:24:31,745] [INFO] ===== Start GTDB Search =====
[2023-03-15 17:24:31,746] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a1857/markers.fasta)
[2023-03-15 17:24:31,747] [INFO] Task started: Blastn
[2023-03-15 17:24:31,747] [INFO] Running command: blastn -query OceanDNA-a1857/markers.fasta -db /var/lib/cwl/stgcd3c2ff8-9f27-48c2-9cc3-add3b91d7e91/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a1857/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 17:24:32,213] [INFO] Task succeeded: Blastn
[2023-03-15 17:24:32,213] [INFO] Selected 10 target genomes.
[2023-03-15 17:24:32,214] [INFO] Target genome list was writen to OceanDNA-a1857/target_genomes_gtdb.txt
[2023-03-15 17:24:32,225] [INFO] Task started: fastANI
[2023-03-15 17:24:32,225] [INFO] Running command: fastANI --query /var/lib/cwl/stg2e5421f8-6d12-4b0a-baa7-ce0c2cc724ff/OceanDNA-a1857.fa --refList OceanDNA-a1857/target_genomes_gtdb.txt --output OceanDNA-a1857/fastani_result_gtdb.tsv --threads 1
[2023-03-15 17:24:35,243] [INFO] Task succeeded: fastANI
[2023-03-15 17:24:35,250] [INFO] Found 6 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 17:24:35,250] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002506705.1	s__MGIIa-K1 sp002506705	81.6463	162	302	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_011525095.1	s__MGIIa-K1 sp011525095	81.428	202	302	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002727695.1	s__MGIIa-K1 sp002727695	77.8765	104	302	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002689565.1	s__MGIIa-K1 sp002689565	77.4131	64	302	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003602415.1	s__MGIIa-K1 sp003602415	77.0063	96	302	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	97.94	97.94	0.93	0.93	2	-
GCA_018650165.1	s__MGIIa-K1 sp018650165	76.4402	56	302	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	99.75	99.69	0.90	0.90	3	-
--------------------------------------------------------------------------------
[2023-03-15 17:24:35,250] [INFO] GTDB search result was written to OceanDNA-a1857/result_gtdb.tsv
[2023-03-15 17:24:35,251] [INFO] ===== GTDB Search completed =====
[2023-03-15 17:24:35,252] [INFO] DFAST_QC result json was written to OceanDNA-a1857/dqc_result.json
[2023-03-15 17:24:35,252] [INFO] DFAST_QC completed!
[2023-03-15 17:24:35,252] [INFO] Total running time: 0h0m34s