[2023-03-16 16:35:56,434] [INFO] DFAST_QC pipeline started.
[2023-03-16 16:35:56,435] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 16:35:56,435] [INFO] DQC Reference Directory: /var/lib/cwl/stgd90a0908-e6d7-4f37-b5b7-82234f51b198/dqc_reference
[2023-03-16 16:35:57,560] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 16:35:57,560] [INFO] Task started: Prodigal
[2023-03-16 16:35:57,561] [INFO] Running command: cat /var/lib/cwl/stg01c8ac03-ecf4-4cc8-9e2d-4213e8812c01/OceanDNA-a1883.fa | prodigal -d OceanDNA-a1883/cds.fna -a OceanDNA-a1883/protein.faa -g 11 -q > /dev/null
[2023-03-16 16:36:09,132] [INFO] Task succeeded: Prodigal
[2023-03-16 16:36:09,133] [INFO] Task started: HMMsearch
[2023-03-16 16:36:09,133] [INFO] Running command: hmmsearch --tblout OceanDNA-a1883/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd90a0908-e6d7-4f37-b5b7-82234f51b198/dqc_reference/reference_markers.hmm OceanDNA-a1883/protein.faa > /dev/null
[2023-03-16 16:36:09,362] [INFO] Task succeeded: HMMsearch
[2023-03-16 16:36:09,362] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg01c8ac03-ecf4-4cc8-9e2d-4213e8812c01/OceanDNA-a1883.fa]
[2023-03-16 16:36:09,376] [INFO] Query marker FASTA was written to OceanDNA-a1883/markers.fasta
[2023-03-16 16:36:09,378] [INFO] Task started: Blastn
[2023-03-16 16:36:09,379] [INFO] Running command: blastn -query OceanDNA-a1883/markers.fasta -db /var/lib/cwl/stgd90a0908-e6d7-4f37-b5b7-82234f51b198/dqc_reference/reference_markers.fasta -out OceanDNA-a1883/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 16:36:09,985] [INFO] Task succeeded: Blastn
[2023-03-16 16:36:09,986] [INFO] Selected 5 target genomes.
[2023-03-16 16:36:09,986] [INFO] Target genome list was writen to OceanDNA-a1883/target_genomes.txt
[2023-03-16 16:36:09,989] [INFO] Task started: fastANI
[2023-03-16 16:36:09,989] [INFO] Running command: fastANI --query /var/lib/cwl/stg01c8ac03-ecf4-4cc8-9e2d-4213e8812c01/OceanDNA-a1883.fa --refList OceanDNA-a1883/target_genomes.txt --output OceanDNA-a1883/fastani_result.tsv --threads 1
[2023-03-16 16:36:12,175] [INFO] Task succeeded: fastANI
[2023-03-16 16:36:12,175] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd90a0908-e6d7-4f37-b5b7-82234f51b198/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 16:36:12,175] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd90a0908-e6d7-4f37-b5b7-82234f51b198/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 16:36:12,175] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 16:36:12,175] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 16:36:12,175] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 16:36:12,176] [INFO] DFAST Taxonomy check result was written to OceanDNA-a1883/tc_result.tsv
[2023-03-16 16:36:12,176] [INFO] ===== Taxonomy check completed =====
[2023-03-16 16:36:12,176] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 16:36:12,176] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd90a0908-e6d7-4f37-b5b7-82234f51b198/dqc_reference/checkm_data
[2023-03-16 16:36:12,188] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 16:36:12,191] [INFO] Task started: CheckM
[2023-03-16 16:36:12,191] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a1883/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a1883/checkm_input OceanDNA-a1883/checkm_result
[2023-03-16 16:36:45,888] [INFO] Task succeeded: CheckM
[2023-03-16 16:36:45,889] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.15%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 16:36:45,891] [INFO] ===== Completeness check finished =====
[2023-03-16 16:36:45,891] [INFO] ===== Start GTDB Search =====
[2023-03-16 16:36:45,891] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a1883/markers.fasta)
[2023-03-16 16:36:45,892] [INFO] Task started: Blastn
[2023-03-16 16:36:45,892] [INFO] Running command: blastn -query OceanDNA-a1883/markers.fasta -db /var/lib/cwl/stgd90a0908-e6d7-4f37-b5b7-82234f51b198/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a1883/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 16:36:46,528] [INFO] Task succeeded: Blastn
[2023-03-16 16:36:46,529] [INFO] Selected 12 target genomes.
[2023-03-16 16:36:46,529] [INFO] Target genome list was writen to OceanDNA-a1883/target_genomes_gtdb.txt
[2023-03-16 16:36:46,658] [INFO] Task started: fastANI
[2023-03-16 16:36:46,658] [INFO] Running command: fastANI --query /var/lib/cwl/stg01c8ac03-ecf4-4cc8-9e2d-4213e8812c01/OceanDNA-a1883.fa --refList OceanDNA-a1883/target_genomes_gtdb.txt --output OceanDNA-a1883/fastani_result_gtdb.tsv --threads 1
[2023-03-16 16:36:50,580] [INFO] Task succeeded: fastANI
[2023-03-16 16:36:50,587] [INFO] Found 11 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 16:36:50,587] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002689565.1	s__MGIIa-K1 sp002689565	93.3097	259	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002710205.1	s__MGIIa-K1 sp002710205	78.8493	193	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002727695.1	s__MGIIa-K1 sp002727695	78.4606	175	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002498185.1	s__MGIIa-K1 sp002498185	78.4068	181	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	99.51	99.39	0.80	0.77	3	-
GCA_002715725.1	s__MGIIa-K1 sp002715725	78.2573	188	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	99.59	99.59	0.80	0.80	2	-
GCA_011525095.1	s__MGIIa-K1 sp011525095	78.0947	139	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003602415.1	s__MGIIa-K1 sp003602415	77.7847	179	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	97.94	97.94	0.93	0.93	2	-
GCA_002720115.1	s__MGIIa-K1 sp002720115	77.5642	159	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002701145.1	s__MGIIa-K1 sp002701145	77.0267	123	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002727515.1	s__MGIIa-K1 sp002727515	76.8895	92	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002694245.1	s__MGIIa-K1 sp002694245	76.6951	111	458	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-K1	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 16:36:50,588] [INFO] GTDB search result was written to OceanDNA-a1883/result_gtdb.tsv
[2023-03-16 16:36:50,588] [INFO] ===== GTDB Search completed =====
[2023-03-16 16:36:50,588] [INFO] DFAST_QC result json was written to OceanDNA-a1883/dqc_result.json
[2023-03-16 16:36:50,589] [INFO] DFAST_QC completed!
[2023-03-16 16:36:50,589] [INFO] Total running time: 0h0m54s