[2023-03-16 06:03:26,036] [INFO] DFAST_QC pipeline started.
[2023-03-16 06:03:26,036] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 06:03:26,036] [INFO] DQC Reference Directory: /var/lib/cwl/stg8895cd20-4955-4ed7-a2cb-27f609fe4a38/dqc_reference
[2023-03-16 06:03:27,146] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 06:03:27,147] [INFO] Task started: Prodigal
[2023-03-16 06:03:27,147] [INFO] Running command: cat /var/lib/cwl/stga3b49795-2aba-4d9d-aa1f-9c03bba0719c/OceanDNA-a2821.fa | prodigal -d OceanDNA-a2821/cds.fna -a OceanDNA-a2821/protein.faa -g 11 -q > /dev/null
[2023-03-16 06:03:32,040] [INFO] Task succeeded: Prodigal
[2023-03-16 06:03:32,040] [INFO] Task started: HMMsearch
[2023-03-16 06:03:32,040] [INFO] Running command: hmmsearch --tblout OceanDNA-a2821/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8895cd20-4955-4ed7-a2cb-27f609fe4a38/dqc_reference/reference_markers.hmm OceanDNA-a2821/protein.faa > /dev/null
[2023-03-16 06:03:32,183] [INFO] Task succeeded: HMMsearch
[2023-03-16 06:03:32,184] [WARNING] Found 1/6 markers. [/var/lib/cwl/stga3b49795-2aba-4d9d-aa1f-9c03bba0719c/OceanDNA-a2821.fa]
[2023-03-16 06:03:32,195] [INFO] Query marker FASTA was written to OceanDNA-a2821/markers.fasta
[2023-03-16 06:03:32,196] [INFO] Task started: Blastn
[2023-03-16 06:03:32,196] [INFO] Running command: blastn -query OceanDNA-a2821/markers.fasta -db /var/lib/cwl/stg8895cd20-4955-4ed7-a2cb-27f609fe4a38/dqc_reference/reference_markers.fasta -out OceanDNA-a2821/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 06:03:32,642] [INFO] Task succeeded: Blastn
[2023-03-16 06:03:32,642] [INFO] Selected 7 target genomes.
[2023-03-16 06:03:32,643] [INFO] Target genome list was writen to OceanDNA-a2821/target_genomes.txt
[2023-03-16 06:03:32,647] [INFO] Task started: fastANI
[2023-03-16 06:03:32,647] [INFO] Running command: fastANI --query /var/lib/cwl/stga3b49795-2aba-4d9d-aa1f-9c03bba0719c/OceanDNA-a2821.fa --refList OceanDNA-a2821/target_genomes.txt --output OceanDNA-a2821/fastani_result.tsv --threads 1
[2023-03-16 06:03:36,362] [INFO] Task succeeded: fastANI
[2023-03-16 06:03:36,362] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8895cd20-4955-4ed7-a2cb-27f609fe4a38/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 06:03:36,363] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8895cd20-4955-4ed7-a2cb-27f609fe4a38/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 06:03:36,363] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 06:03:36,363] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 06:03:36,363] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 06:03:36,363] [INFO] DFAST Taxonomy check result was written to OceanDNA-a2821/tc_result.tsv
[2023-03-16 06:03:36,363] [INFO] ===== Taxonomy check completed =====
[2023-03-16 06:03:36,363] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 06:03:36,363] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8895cd20-4955-4ed7-a2cb-27f609fe4a38/dqc_reference/checkm_data
[2023-03-16 06:03:36,366] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 06:03:36,369] [INFO] Task started: CheckM
[2023-03-16 06:03:36,369] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a2821/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a2821/checkm_input OceanDNA-a2821/checkm_result
[2023-03-16 06:03:55,257] [INFO] Task succeeded: CheckM
[2023-03-16 06:03:55,258] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 62.57%
Contamintation: 0.38%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 06:03:55,288] [INFO] ===== Completeness check finished =====
[2023-03-16 06:03:55,288] [INFO] ===== Start GTDB Search =====
[2023-03-16 06:03:55,288] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a2821/markers.fasta)
[2023-03-16 06:03:55,288] [INFO] Task started: Blastn
[2023-03-16 06:03:55,288] [INFO] Running command: blastn -query OceanDNA-a2821/markers.fasta -db /var/lib/cwl/stg8895cd20-4955-4ed7-a2cb-27f609fe4a38/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a2821/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 06:03:55,806] [INFO] Task succeeded: Blastn
[2023-03-16 06:03:55,807] [INFO] Selected 5 target genomes.
[2023-03-16 06:03:55,807] [INFO] Target genome list was writen to OceanDNA-a2821/target_genomes_gtdb.txt
[2023-03-16 06:03:55,825] [INFO] Task started: fastANI
[2023-03-16 06:03:55,825] [INFO] Running command: fastANI --query /var/lib/cwl/stga3b49795-2aba-4d9d-aa1f-9c03bba0719c/OceanDNA-a2821.fa --refList OceanDNA-a2821/target_genomes_gtdb.txt --output OceanDNA-a2821/fastani_result_gtdb.tsv --threads 1
[2023-03-16 06:03:57,330] [INFO] Task succeeded: fastANI
[2023-03-16 06:03:57,334] [INFO] Found 5 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 06:03:57,334] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018645535.1	s__MGIIa-L2 sp018645535	92.5234	138	187	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L2	95.0	N/A	N/A	N/A	N/A	1	-
GCA_009887135.1	s__MGIIa-L2 sp009887135	88.3135	167	187	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L2	95.0	98.87	98.45	0.83	0.81	3	-
GCA_016777125.1	s__MGIIa-L2 sp016777125	87.989	180	187	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L2	95.0	99.94	99.94	0.96	0.96	2	-
GCA_003602665.1	s__MGIIa-L2 sp003602665	85.1376	163	187	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L2	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018666575.1	s__MGIIa-L2 sp018666575	83.4673	147	187	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L2	95.0	99.20	99.20	0.82	0.82	2	-
--------------------------------------------------------------------------------
[2023-03-16 06:03:57,336] [INFO] GTDB search result was written to OceanDNA-a2821/result_gtdb.tsv
[2023-03-16 06:03:57,336] [INFO] ===== GTDB Search completed =====
[2023-03-16 06:03:57,337] [INFO] DFAST_QC result json was written to OceanDNA-a2821/dqc_result.json
[2023-03-16 06:03:57,337] [INFO] DFAST_QC completed!
[2023-03-16 06:03:57,337] [INFO] Total running time: 0h0m31s