[2023-03-17 09:46:50,397] [INFO] DFAST_QC pipeline started.
[2023-03-17 09:46:50,399] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 09:46:50,399] [INFO] DQC Reference Directory: /var/lib/cwl/stg7168c08c-aac0-4936-b187-8eae0edf6a9b/dqc_reference
[2023-03-17 09:46:51,528] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 09:46:51,530] [INFO] Task started: Prodigal
[2023-03-17 09:46:51,530] [INFO] Running command: cat /var/lib/cwl/stgf4b0ab41-e899-4245-b881-cbaaf5c454a7/OceanDNA-a2910.fa | prodigal -d OceanDNA-a2910/cds.fna -a OceanDNA-a2910/protein.faa -g 11 -q > /dev/null
[2023-03-17 09:47:03,519] [INFO] Task succeeded: Prodigal
[2023-03-17 09:47:03,520] [INFO] Task started: HMMsearch
[2023-03-17 09:47:03,520] [INFO] Running command: hmmsearch --tblout OceanDNA-a2910/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg7168c08c-aac0-4936-b187-8eae0edf6a9b/dqc_reference/reference_markers.hmm OceanDNA-a2910/protein.faa > /dev/null
[2023-03-17 09:47:03,714] [INFO] Task succeeded: HMMsearch
[2023-03-17 09:47:03,715] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgf4b0ab41-e899-4245-b881-cbaaf5c454a7/OceanDNA-a2910.fa]
[2023-03-17 09:47:03,750] [INFO] Query marker FASTA was written to OceanDNA-a2910/markers.fasta
[2023-03-17 09:47:03,751] [INFO] Task started: Blastn
[2023-03-17 09:47:03,751] [INFO] Running command: blastn -query OceanDNA-a2910/markers.fasta -db /var/lib/cwl/stg7168c08c-aac0-4936-b187-8eae0edf6a9b/dqc_reference/reference_markers.fasta -out OceanDNA-a2910/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 09:47:04,413] [INFO] Task succeeded: Blastn
[2023-03-17 09:47:04,425] [INFO] Selected 5 target genomes.
[2023-03-17 09:47:04,425] [INFO] Target genome list was writen to OceanDNA-a2910/target_genomes.txt
[2023-03-17 09:47:04,427] [INFO] Task started: fastANI
[2023-03-17 09:47:04,428] [INFO] Running command: fastANI --query /var/lib/cwl/stgf4b0ab41-e899-4245-b881-cbaaf5c454a7/OceanDNA-a2910.fa --refList OceanDNA-a2910/target_genomes.txt --output OceanDNA-a2910/fastani_result.tsv --threads 1
[2023-03-17 09:47:07,189] [INFO] Task succeeded: fastANI
[2023-03-17 09:47:07,189] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg7168c08c-aac0-4936-b187-8eae0edf6a9b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 09:47:07,189] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg7168c08c-aac0-4936-b187-8eae0edf6a9b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 09:47:07,190] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 09:47:07,190] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 09:47:07,190] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 09:47:07,194] [INFO] DFAST Taxonomy check result was written to OceanDNA-a2910/tc_result.tsv
[2023-03-17 09:47:07,198] [INFO] ===== Taxonomy check completed =====
[2023-03-17 09:47:07,198] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 09:47:07,198] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg7168c08c-aac0-4936-b187-8eae0edf6a9b/dqc_reference/checkm_data
[2023-03-17 09:47:07,256] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 09:47:07,361] [INFO] Task started: CheckM
[2023-03-17 09:47:07,361] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a2910/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a2910/checkm_input OceanDNA-a2910/checkm_result
[2023-03-17 09:47:58,001] [INFO] Task succeeded: CheckM
[2023-03-17 09:47:58,001] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 65.66%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-17 09:47:58,087] [INFO] ===== Completeness check finished =====
[2023-03-17 09:47:58,087] [INFO] ===== Start GTDB Search =====
[2023-03-17 09:47:58,087] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a2910/markers.fasta)
[2023-03-17 09:47:58,102] [INFO] Task started: Blastn
[2023-03-17 09:47:58,102] [INFO] Running command: blastn -query OceanDNA-a2910/markers.fasta -db /var/lib/cwl/stg7168c08c-aac0-4936-b187-8eae0edf6a9b/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a2910/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 09:47:58,973] [INFO] Task succeeded: Blastn
[2023-03-17 09:47:58,982] [INFO] Selected 6 target genomes.
[2023-03-17 09:47:58,982] [INFO] Target genome list was writen to OceanDNA-a2910/target_genomes_gtdb.txt
[2023-03-17 09:47:59,034] [INFO] Task started: fastANI
[2023-03-17 09:47:59,034] [INFO] Running command: fastANI --query /var/lib/cwl/stgf4b0ab41-e899-4245-b881-cbaaf5c454a7/OceanDNA-a2910.fa --refList OceanDNA-a2910/target_genomes_gtdb.txt --output OceanDNA-a2910/fastani_result_gtdb.tsv --threads 1
[2023-03-17 09:48:01,030] [INFO] Task succeeded: fastANI
[2023-03-17 09:48:01,035] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-17 09:48:01,035] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_013330925.1	s__MGIIa-L3 sp11892u	99.3473	397	552	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L3	95.0	99.55	99.23	0.93	0.88	5	conclusive
GCA_013330765.1	s__MGIIa-L3 sp12206u	83.8047	279	552	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L3	95.0	100.00	100.00	0.99	0.99	2	-
GCA_002722135.1	s__MGIIa-L3 sp002722135	81.7059	389	552	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L3	95.0	99.74	99.72	0.92	0.89	4	-
GCA_002694585.1	s__MGIIa-L3 sp002694585	81.5462	312	552	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L3	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002731395.1	s__MGIIa-L3 sp002731395	80.8085	260	552	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L3	95.0	99.15	99.02	0.81	0.76	3	-
GCA_002497765.1	s__Poseidonia sp002497765	77.0705	67	552	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	99.70	99.65	0.88	0.86	3	-
--------------------------------------------------------------------------------
[2023-03-17 09:48:01,038] [INFO] GTDB search result was written to OceanDNA-a2910/result_gtdb.tsv
[2023-03-17 09:48:01,048] [INFO] ===== GTDB Search completed =====
[2023-03-17 09:48:01,055] [INFO] DFAST_QC result json was written to OceanDNA-a2910/dqc_result.json
[2023-03-17 09:48:01,055] [INFO] DFAST_QC completed!
[2023-03-17 09:48:01,055] [INFO] Total running time: 0h1m11s