[2023-03-16 20:25:54,869] [INFO] DFAST_QC pipeline started.
[2023-03-16 20:25:54,870] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 20:25:54,870] [INFO] DQC Reference Directory: /var/lib/cwl/stge01c6182-ac09-4534-a57c-ce981432f42f/dqc_reference
[2023-03-16 20:25:55,973] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 20:25:55,974] [INFO] Task started: Prodigal
[2023-03-16 20:25:55,974] [INFO] Running command: cat /var/lib/cwl/stg480dc82c-6ae5-4052-bfda-0c73bf69bedb/OceanDNA-a2924.fa | prodigal -d OceanDNA-a2924/cds.fna -a OceanDNA-a2924/protein.faa -g 11 -q > /dev/null
[2023-03-16 20:26:05,670] [INFO] Task succeeded: Prodigal
[2023-03-16 20:26:05,670] [INFO] Task started: HMMsearch
[2023-03-16 20:26:05,670] [INFO] Running command: hmmsearch --tblout OceanDNA-a2924/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge01c6182-ac09-4534-a57c-ce981432f42f/dqc_reference/reference_markers.hmm OceanDNA-a2924/protein.faa > /dev/null
[2023-03-16 20:26:05,832] [INFO] Task succeeded: HMMsearch
[2023-03-16 20:26:05,832] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg480dc82c-6ae5-4052-bfda-0c73bf69bedb/OceanDNA-a2924.fa]
[2023-03-16 20:26:05,844] [INFO] Query marker FASTA was written to OceanDNA-a2924/markers.fasta
[2023-03-16 20:26:05,845] [INFO] Task started: Blastn
[2023-03-16 20:26:05,845] [INFO] Running command: blastn -query OceanDNA-a2924/markers.fasta -db /var/lib/cwl/stge01c6182-ac09-4534-a57c-ce981432f42f/dqc_reference/reference_markers.fasta -out OceanDNA-a2924/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 20:26:06,316] [INFO] Task succeeded: Blastn
[2023-03-16 20:26:06,317] [INFO] Selected 5 target genomes.
[2023-03-16 20:26:06,317] [INFO] Target genome list was writen to OceanDNA-a2924/target_genomes.txt
[2023-03-16 20:26:06,324] [INFO] Task started: fastANI
[2023-03-16 20:26:06,324] [INFO] Running command: fastANI --query /var/lib/cwl/stg480dc82c-6ae5-4052-bfda-0c73bf69bedb/OceanDNA-a2924.fa --refList OceanDNA-a2924/target_genomes.txt --output OceanDNA-a2924/fastani_result.tsv --threads 1
[2023-03-16 20:26:09,320] [INFO] Task succeeded: fastANI
[2023-03-16 20:26:09,320] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge01c6182-ac09-4534-a57c-ce981432f42f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 20:26:09,320] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge01c6182-ac09-4534-a57c-ce981432f42f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 20:26:09,320] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 20:26:09,320] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 20:26:09,320] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 20:26:09,321] [INFO] DFAST Taxonomy check result was written to OceanDNA-a2924/tc_result.tsv
[2023-03-16 20:26:09,321] [INFO] ===== Taxonomy check completed =====
[2023-03-16 20:26:09,321] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 20:26:09,321] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge01c6182-ac09-4534-a57c-ce981432f42f/dqc_reference/checkm_data
[2023-03-16 20:26:09,331] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 20:26:09,777] [INFO] Task started: CheckM
[2023-03-16 20:26:09,777] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a2924/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a2924/checkm_input OceanDNA-a2924/checkm_result
[2023-03-16 20:26:47,636] [INFO] Task succeeded: CheckM
[2023-03-16 20:26:47,637] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.94%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 20:26:47,640] [INFO] ===== Completeness check finished =====
[2023-03-16 20:26:47,641] [INFO] ===== Start GTDB Search =====
[2023-03-16 20:26:47,641] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a2924/markers.fasta)
[2023-03-16 20:26:47,643] [INFO] Task started: Blastn
[2023-03-16 20:26:47,643] [INFO] Running command: blastn -query OceanDNA-a2924/markers.fasta -db /var/lib/cwl/stge01c6182-ac09-4534-a57c-ce981432f42f/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a2924/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 20:26:48,361] [INFO] Task succeeded: Blastn
[2023-03-16 20:26:48,364] [INFO] Selected 9 target genomes.
[2023-03-16 20:26:48,364] [INFO] Target genome list was writen to OceanDNA-a2924/target_genomes_gtdb.txt
[2023-03-16 20:26:48,375] [INFO] Task started: fastANI
[2023-03-16 20:26:48,376] [INFO] Running command: fastANI --query /var/lib/cwl/stg480dc82c-6ae5-4052-bfda-0c73bf69bedb/OceanDNA-a2924.fa --refList OceanDNA-a2924/target_genomes_gtdb.txt --output OceanDNA-a2924/fastani_result_gtdb.tsv --threads 1
[2023-03-16 20:26:51,984] [INFO] Task succeeded: fastANI
[2023-03-16 20:26:51,989] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 20:26:51,990] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002718195.1	s__MGIIa-L4 sp002718195	99.8582	490	570	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__MGIIa-L4	95.0	99.84	99.84	0.65	0.65	2	conclusive
--------------------------------------------------------------------------------
[2023-03-16 20:26:51,990] [INFO] GTDB search result was written to OceanDNA-a2924/result_gtdb.tsv
[2023-03-16 20:26:51,990] [INFO] ===== GTDB Search completed =====
[2023-03-16 20:26:51,991] [INFO] DFAST_QC result json was written to OceanDNA-a2924/dqc_result.json
[2023-03-16 20:26:51,991] [INFO] DFAST_QC completed!
[2023-03-16 20:26:51,991] [INFO] Total running time: 0h0m57s