[2023-03-18 05:53:16,848] [INFO] DFAST_QC pipeline started.
[2023-03-18 05:53:16,848] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 05:53:16,848] [INFO] DQC Reference Directory: /var/lib/cwl/stg74f3ec76-7a7e-4d7c-bbcb-b0413f0291ce/dqc_reference
[2023-03-18 05:53:17,979] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 05:53:17,979] [INFO] Task started: Prodigal
[2023-03-18 05:53:17,980] [INFO] Running command: cat /var/lib/cwl/stg85328628-287d-4b80-ab02-2f2fd0b3298c/OceanDNA-a2983.fa | prodigal -d OceanDNA-a2983/cds.fna -a OceanDNA-a2983/protein.faa -g 11 -q > /dev/null
[2023-03-18 05:53:26,188] [INFO] Task succeeded: Prodigal
[2023-03-18 05:53:26,188] [INFO] Task started: HMMsearch
[2023-03-18 05:53:26,188] [INFO] Running command: hmmsearch --tblout OceanDNA-a2983/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg74f3ec76-7a7e-4d7c-bbcb-b0413f0291ce/dqc_reference/reference_markers.hmm OceanDNA-a2983/protein.faa > /dev/null
[2023-03-18 05:53:26,338] [INFO] Task succeeded: HMMsearch
[2023-03-18 05:53:26,339] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg85328628-287d-4b80-ab02-2f2fd0b3298c/OceanDNA-a2983.fa]
[2023-03-18 05:53:26,350] [INFO] Query marker FASTA was written to OceanDNA-a2983/markers.fasta
[2023-03-18 05:53:26,350] [INFO] Task started: Blastn
[2023-03-18 05:53:26,350] [INFO] Running command: blastn -query OceanDNA-a2983/markers.fasta -db /var/lib/cwl/stg74f3ec76-7a7e-4d7c-bbcb-b0413f0291ce/dqc_reference/reference_markers.fasta -out OceanDNA-a2983/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 05:53:26,783] [INFO] Task succeeded: Blastn
[2023-03-18 05:53:26,784] [INFO] Selected 5 target genomes.
[2023-03-18 05:53:26,784] [INFO] Target genome list was writen to OceanDNA-a2983/target_genomes.txt
[2023-03-18 05:53:26,787] [INFO] Task started: fastANI
[2023-03-18 05:53:26,787] [INFO] Running command: fastANI --query /var/lib/cwl/stg85328628-287d-4b80-ab02-2f2fd0b3298c/OceanDNA-a2983.fa --refList OceanDNA-a2983/target_genomes.txt --output OceanDNA-a2983/fastani_result.tsv --threads 1
[2023-03-18 05:53:30,374] [INFO] Task succeeded: fastANI
[2023-03-18 05:53:30,374] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg74f3ec76-7a7e-4d7c-bbcb-b0413f0291ce/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 05:53:30,375] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg74f3ec76-7a7e-4d7c-bbcb-b0413f0291ce/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 05:53:30,375] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 05:53:30,375] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 05:53:30,375] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 05:53:30,375] [INFO] DFAST Taxonomy check result was written to OceanDNA-a2983/tc_result.tsv
[2023-03-18 05:53:30,375] [INFO] ===== Taxonomy check completed =====
[2023-03-18 05:53:30,375] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 05:53:30,375] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg74f3ec76-7a7e-4d7c-bbcb-b0413f0291ce/dqc_reference/checkm_data
[2023-03-18 05:53:30,378] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 05:53:30,381] [INFO] Task started: CheckM
[2023-03-18 05:53:30,382] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a2983/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a2983/checkm_input OceanDNA-a2983/checkm_result
[2023-03-18 05:53:56,759] [INFO] Task succeeded: CheckM
[2023-03-18 05:53:56,759] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 71.21%
Contamintation: 1.04%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 05:53:56,790] [INFO] ===== Completeness check finished =====
[2023-03-18 05:53:56,790] [INFO] ===== Start GTDB Search =====
[2023-03-18 05:53:56,790] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a2983/markers.fasta)
[2023-03-18 05:53:56,791] [INFO] Task started: Blastn
[2023-03-18 05:53:56,792] [INFO] Running command: blastn -query OceanDNA-a2983/markers.fasta -db /var/lib/cwl/stg74f3ec76-7a7e-4d7c-bbcb-b0413f0291ce/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a2983/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 05:53:57,236] [INFO] Task succeeded: Blastn
[2023-03-18 05:53:57,236] [INFO] Selected 10 target genomes.
[2023-03-18 05:53:57,237] [INFO] Target genome list was writen to OceanDNA-a2983/target_genomes_gtdb.txt
[2023-03-18 05:53:57,261] [INFO] Task started: fastANI
[2023-03-18 05:53:57,261] [INFO] Running command: fastANI --query /var/lib/cwl/stg85328628-287d-4b80-ab02-2f2fd0b3298c/OceanDNA-a2983.fa --refList OceanDNA-a2983/target_genomes_gtdb.txt --output OceanDNA-a2983/fastani_result_gtdb.tsv --threads 1
[2023-03-18 05:54:00,291] [INFO] Task succeeded: fastANI
[2023-03-18 05:54:00,296] [INFO] Found 9 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 05:54:00,297] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002495645.1	s__Poseidonia sp002495645	92.3402	325	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	99.68	99.68	0.84	0.84	2	-
GCA_002689105.1	s__Poseidonia sp002689105	81.8194	270	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	96.72	96.72	0.80	0.80	2	-
GCA_002497765.1	s__Poseidonia sp002497765	79.936	201	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	99.70	99.65	0.88	0.86	3	-
GCA_002714305.1	s__Poseidonia sp002714305	78.9393	160	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	98.69	98.28	0.79	0.76	8	-
GCA_002694525.1	s__Poseidonia sp002694525	78.5529	132	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002502295.1	s__Poseidonia sp002502295	78.4804	143	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013330155.1	s__Poseidonia sp8339u	78.3714	151	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	99.08	98.16	0.89	0.82	3	-
GCA_002722695.1	s__Poseidonia sp002722695	78.3476	140	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	99.22	99.22	0.79	0.79	3	-
GCA_016779705.1	s__Poseidonia sp016779705	78.2694	121	368	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__Poseidonia	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 05:54:00,297] [INFO] GTDB search result was written to OceanDNA-a2983/result_gtdb.tsv
[2023-03-18 05:54:00,297] [INFO] ===== GTDB Search completed =====
[2023-03-18 05:54:00,298] [INFO] DFAST_QC result json was written to OceanDNA-a2983/dqc_result.json
[2023-03-18 05:54:00,298] [INFO] DFAST_QC completed!
[2023-03-18 05:54:00,298] [INFO] Total running time: 0h0m43s