[2023-03-14 14:35:17,469] [INFO] DFAST_QC pipeline started.
[2023-03-14 14:35:17,469] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 14:35:17,469] [INFO] DQC Reference Directory: /var/lib/cwl/stg8fae0c58-78c7-41c1-95f6-081e5aaf222a/dqc_reference
[2023-03-14 14:35:20,301] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 14:35:20,302] [INFO] Task started: Prodigal
[2023-03-14 14:35:20,302] [INFO] Running command: cat /var/lib/cwl/stg39831049-c342-4055-94d1-c667c299636b/OceanDNA-a3157.fa | prodigal -d OceanDNA-a3157/cds.fna -a OceanDNA-a3157/protein.faa -g 11 -q > /dev/null
[2023-03-14 14:35:30,225] [INFO] Task succeeded: Prodigal
[2023-03-14 14:35:30,226] [INFO] Task started: HMMsearch
[2023-03-14 14:35:30,226] [INFO] Running command: hmmsearch --tblout OceanDNA-a3157/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8fae0c58-78c7-41c1-95f6-081e5aaf222a/dqc_reference/reference_markers.hmm OceanDNA-a3157/protein.faa > /dev/null
[2023-03-14 14:35:30,397] [INFO] Task succeeded: HMMsearch
[2023-03-14 14:35:30,397] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg39831049-c342-4055-94d1-c667c299636b/OceanDNA-a3157.fa]
[2023-03-14 14:35:30,409] [INFO] Query marker FASTA was written to OceanDNA-a3157/markers.fasta
[2023-03-14 14:35:30,410] [INFO] Task started: Blastn
[2023-03-14 14:35:30,410] [INFO] Running command: blastn -query OceanDNA-a3157/markers.fasta -db /var/lib/cwl/stg8fae0c58-78c7-41c1-95f6-081e5aaf222a/dqc_reference/reference_markers.fasta -out OceanDNA-a3157/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 14:35:30,831] [INFO] Task succeeded: Blastn
[2023-03-14 14:35:30,832] [INFO] Selected 0 target genomes.
[2023-03-14 14:35:30,832] [INFO] Target genome list was writen to OceanDNA-a3157/target_genomes.txt
[2023-03-14 14:35:30,832] [ERROR] File is empty. [OceanDNA-a3157/target_genomes.txt]
[2023-03-14 14:35:30,833] [ERROR] Task failed. No target genome found.
[2023-03-14 14:35:30,833] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 14:35:30,833] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8fae0c58-78c7-41c1-95f6-081e5aaf222a/dqc_reference/checkm_data
[2023-03-14 14:35:30,889] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 14:35:30,893] [INFO] Task started: CheckM
[2023-03-14 14:35:30,893] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a3157/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a3157/checkm_input OceanDNA-a3157/checkm_result
[2023-03-14 14:36:03,865] [INFO] Task succeeded: CheckM
[2023-03-14 14:36:03,865] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.94%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-14 14:36:03,867] [INFO] ===== Completeness check finished =====
[2023-03-14 14:36:03,868] [INFO] ===== Start GTDB Search =====
[2023-03-14 14:36:03,868] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a3157/markers.fasta)
[2023-03-14 14:36:03,869] [INFO] Task started: Blastn
[2023-03-14 14:36:03,869] [INFO] Running command: blastn -query OceanDNA-a3157/markers.fasta -db /var/lib/cwl/stg8fae0c58-78c7-41c1-95f6-081e5aaf222a/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a3157/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 14:36:04,473] [INFO] Task succeeded: Blastn
[2023-03-14 14:36:04,474] [INFO] Selected 9 target genomes.
[2023-03-14 14:36:04,475] [INFO] Target genome list was writen to OceanDNA-a3157/target_genomes_gtdb.txt
[2023-03-14 14:36:04,493] [INFO] Task started: fastANI
[2023-03-14 14:36:04,493] [INFO] Running command: fastANI --query /var/lib/cwl/stg39831049-c342-4055-94d1-c667c299636b/OceanDNA-a3157.fa --refList OceanDNA-a3157/target_genomes_gtdb.txt --output OceanDNA-a3157/fastani_result_gtdb.tsv --threads 1
[2023-03-14 14:36:07,013] [INFO] Task succeeded: fastANI
[2023-03-14 14:36:07,014] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-14 14:36:07,014] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-14 14:36:07,014] [INFO] GTDB search result was written to OceanDNA-a3157/result_gtdb.tsv
[2023-03-14 14:36:07,014] [INFO] ===== GTDB Search completed =====
[2023-03-14 14:36:07,015] [INFO] DFAST_QC result json was written to OceanDNA-a3157/dqc_result.json
[2023-03-14 14:36:07,015] [INFO] DFAST_QC completed!
[2023-03-14 14:36:07,015] [INFO] Total running time: 0h0m50s