[2023-03-16 23:05:03,841] [INFO] DFAST_QC pipeline started.
[2023-03-16 23:05:03,841] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 23:05:03,841] [INFO] DQC Reference Directory: /var/lib/cwl/stgae7e7363-1735-4603-acb3-c3c3ab11c78c/dqc_reference
[2023-03-16 23:05:05,097] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 23:05:05,097] [INFO] Task started: Prodigal
[2023-03-16 23:05:05,097] [INFO] Running command: cat /var/lib/cwl/stg64934eaf-7ba2-4eb7-a08c-048a73091cbe/OceanDNA-a3165.fa | prodigal -d OceanDNA-a3165/cds.fna -a OceanDNA-a3165/protein.faa -g 11 -q > /dev/null
[2023-03-16 23:05:13,078] [INFO] Task succeeded: Prodigal
[2023-03-16 23:05:13,079] [INFO] Task started: HMMsearch
[2023-03-16 23:05:13,079] [INFO] Running command: hmmsearch --tblout OceanDNA-a3165/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgae7e7363-1735-4603-acb3-c3c3ab11c78c/dqc_reference/reference_markers.hmm OceanDNA-a3165/protein.faa > /dev/null
[2023-03-16 23:05:13,232] [INFO] Task succeeded: HMMsearch
[2023-03-16 23:05:13,233] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg64934eaf-7ba2-4eb7-a08c-048a73091cbe/OceanDNA-a3165.fa]
[2023-03-16 23:05:13,245] [INFO] Query marker FASTA was written to OceanDNA-a3165/markers.fasta
[2023-03-16 23:05:13,246] [INFO] Task started: Blastn
[2023-03-16 23:05:13,246] [INFO] Running command: blastn -query OceanDNA-a3165/markers.fasta -db /var/lib/cwl/stgae7e7363-1735-4603-acb3-c3c3ab11c78c/dqc_reference/reference_markers.fasta -out OceanDNA-a3165/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:05:13,764] [INFO] Task succeeded: Blastn
[2023-03-16 23:05:13,764] [INFO] Selected 6 target genomes.
[2023-03-16 23:05:13,765] [INFO] Target genome list was writen to OceanDNA-a3165/target_genomes.txt
[2023-03-16 23:05:13,768] [INFO] Task started: fastANI
[2023-03-16 23:05:13,768] [INFO] Running command: fastANI --query /var/lib/cwl/stg64934eaf-7ba2-4eb7-a08c-048a73091cbe/OceanDNA-a3165.fa --refList OceanDNA-a3165/target_genomes.txt --output OceanDNA-a3165/fastani_result.tsv --threads 1
[2023-03-16 23:05:18,248] [INFO] Task succeeded: fastANI
[2023-03-16 23:05:18,249] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgae7e7363-1735-4603-acb3-c3c3ab11c78c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 23:05:18,249] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgae7e7363-1735-4603-acb3-c3c3ab11c78c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 23:05:18,249] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 23:05:18,249] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 23:05:18,249] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 23:05:18,254] [INFO] DFAST Taxonomy check result was written to OceanDNA-a3165/tc_result.tsv
[2023-03-16 23:05:18,254] [INFO] ===== Taxonomy check completed =====
[2023-03-16 23:05:18,254] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 23:05:18,255] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgae7e7363-1735-4603-acb3-c3c3ab11c78c/dqc_reference/checkm_data
[2023-03-16 23:05:18,257] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 23:05:18,261] [INFO] Task started: CheckM
[2023-03-16 23:05:18,261] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a3165/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a3165/checkm_input OceanDNA-a3165/checkm_result
[2023-03-16 23:05:43,876] [INFO] Task succeeded: CheckM
[2023-03-16 23:05:43,877] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.94%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 23:05:43,879] [INFO] ===== Completeness check finished =====
[2023-03-16 23:05:43,879] [INFO] ===== Start GTDB Search =====
[2023-03-16 23:05:43,879] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a3165/markers.fasta)
[2023-03-16 23:05:43,879] [INFO] Task started: Blastn
[2023-03-16 23:05:43,879] [INFO] Running command: blastn -query OceanDNA-a3165/markers.fasta -db /var/lib/cwl/stgae7e7363-1735-4603-acb3-c3c3ab11c78c/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a3165/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:05:44,498] [INFO] Task succeeded: Blastn
[2023-03-16 23:05:44,499] [INFO] Selected 10 target genomes.
[2023-03-16 23:05:44,499] [INFO] Target genome list was writen to OceanDNA-a3165/target_genomes_gtdb.txt
[2023-03-16 23:05:44,739] [INFO] Task started: fastANI
[2023-03-16 23:05:44,739] [INFO] Running command: fastANI --query /var/lib/cwl/stg64934eaf-7ba2-4eb7-a08c-048a73091cbe/OceanDNA-a3165.fa --refList OceanDNA-a3165/target_genomes_gtdb.txt --output OceanDNA-a3165/fastani_result_gtdb.tsv --threads 1
[2023-03-16 23:05:50,257] [INFO] Task succeeded: fastANI
[2023-03-16 23:05:50,259] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 23:05:50,259] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002503395.1	s__UBA60 sp002503395	85.104	365	420	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Poseidoniaceae;g__UBA60	95.0	96.30	96.26	0.88	0.85	5	-
--------------------------------------------------------------------------------
[2023-03-16 23:05:50,259] [INFO] GTDB search result was written to OceanDNA-a3165/result_gtdb.tsv
[2023-03-16 23:05:50,259] [INFO] ===== GTDB Search completed =====
[2023-03-16 23:05:50,260] [INFO] DFAST_QC result json was written to OceanDNA-a3165/dqc_result.json
[2023-03-16 23:05:50,260] [INFO] DFAST_QC completed!
[2023-03-16 23:05:50,260] [INFO] Total running time: 0h0m46s