[2023-03-14 12:43:36,764] [INFO] DFAST_QC pipeline started.
[2023-03-14 12:43:36,764] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 12:43:36,765] [INFO] DQC Reference Directory: /var/lib/cwl/stgfbfd750b-c057-44ce-9cb8-a97ecc4397cd/dqc_reference
[2023-03-14 12:43:40,857] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 12:43:40,858] [INFO] Task started: Prodigal
[2023-03-14 12:43:40,858] [INFO] Running command: cat /var/lib/cwl/stgfbfb64c4-5d98-46b9-b6e8-c3e1757445c3/OceanDNA-a3221.fa | prodigal -d OceanDNA-a3221/cds.fna -a OceanDNA-a3221/protein.faa -g 11 -q > /dev/null
[2023-03-14 12:43:48,911] [INFO] Task succeeded: Prodigal
[2023-03-14 12:43:48,912] [INFO] Task started: HMMsearch
[2023-03-14 12:43:48,912] [INFO] Running command: hmmsearch --tblout OceanDNA-a3221/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfbfd750b-c057-44ce-9cb8-a97ecc4397cd/dqc_reference/reference_markers.hmm OceanDNA-a3221/protein.faa > /dev/null
[2023-03-14 12:43:49,095] [INFO] Task succeeded: HMMsearch
[2023-03-14 12:43:49,095] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgfbfb64c4-5d98-46b9-b6e8-c3e1757445c3/OceanDNA-a3221.fa]
[2023-03-14 12:43:49,108] [INFO] Query marker FASTA was written to OceanDNA-a3221/markers.fasta
[2023-03-14 12:43:49,109] [INFO] Task started: Blastn
[2023-03-14 12:43:49,109] [INFO] Running command: blastn -query OceanDNA-a3221/markers.fasta -db /var/lib/cwl/stgfbfd750b-c057-44ce-9cb8-a97ecc4397cd/dqc_reference/reference_markers.fasta -out OceanDNA-a3221/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 12:43:49,597] [INFO] Task succeeded: Blastn
[2023-03-14 12:43:49,598] [INFO] Selected 5 target genomes.
[2023-03-14 12:43:49,598] [INFO] Target genome list was writen to OceanDNA-a3221/target_genomes.txt
[2023-03-14 12:43:49,606] [INFO] Task started: fastANI
[2023-03-14 12:43:49,606] [INFO] Running command: fastANI --query /var/lib/cwl/stgfbfb64c4-5d98-46b9-b6e8-c3e1757445c3/OceanDNA-a3221.fa --refList OceanDNA-a3221/target_genomes.txt --output OceanDNA-a3221/fastani_result.tsv --threads 1
[2023-03-14 12:43:54,637] [INFO] Task succeeded: fastANI
[2023-03-14 12:43:54,638] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgfbfd750b-c057-44ce-9cb8-a97ecc4397cd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 12:43:54,638] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgfbfd750b-c057-44ce-9cb8-a97ecc4397cd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 12:43:54,638] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 12:43:54,638] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-14 12:43:54,638] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-14 12:43:54,639] [INFO] DFAST Taxonomy check result was written to OceanDNA-a3221/tc_result.tsv
[2023-03-14 12:43:54,640] [INFO] ===== Taxonomy check completed =====
[2023-03-14 12:43:54,640] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 12:43:54,640] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfbfd750b-c057-44ce-9cb8-a97ecc4397cd/dqc_reference/checkm_data
[2023-03-14 12:43:54,652] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 12:43:54,656] [INFO] Task started: CheckM
[2023-03-14 12:43:54,657] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a3221/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a3221/checkm_input OceanDNA-a3221/checkm_result
[2023-03-14 12:44:24,410] [INFO] Task succeeded: CheckM
[2023-03-14 12:44:24,410] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 76.94%
Contamintation: 0.46%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-14 12:44:24,415] [INFO] ===== Completeness check finished =====
[2023-03-14 12:44:24,415] [INFO] ===== Start GTDB Search =====
[2023-03-14 12:44:24,415] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a3221/markers.fasta)
[2023-03-14 12:44:24,417] [INFO] Task started: Blastn
[2023-03-14 12:44:24,417] [INFO] Running command: blastn -query OceanDNA-a3221/markers.fasta -db /var/lib/cwl/stgfbfd750b-c057-44ce-9cb8-a97ecc4397cd/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a3221/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 12:44:25,140] [INFO] Task succeeded: Blastn
[2023-03-14 12:44:25,141] [INFO] Selected 5 target genomes.
[2023-03-14 12:44:25,141] [INFO] Target genome list was writen to OceanDNA-a3221/target_genomes_gtdb.txt
[2023-03-14 12:44:25,144] [INFO] Task started: fastANI
[2023-03-14 12:44:25,144] [INFO] Running command: fastANI --query /var/lib/cwl/stgfbfb64c4-5d98-46b9-b6e8-c3e1757445c3/OceanDNA-a3221.fa --refList OceanDNA-a3221/target_genomes_gtdb.txt --output OceanDNA-a3221/fastani_result_gtdb.tsv --threads 1
[2023-03-14 12:44:27,101] [INFO] Task succeeded: fastANI
[2023-03-14 12:44:27,101] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-14 12:44:27,102] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-14 12:44:27,104] [INFO] GTDB search result was written to OceanDNA-a3221/result_gtdb.tsv
[2023-03-14 12:44:27,104] [INFO] ===== GTDB Search completed =====
[2023-03-14 12:44:27,105] [INFO] DFAST_QC result json was written to OceanDNA-a3221/dqc_result.json
[2023-03-14 12:44:27,105] [INFO] DFAST_QC completed!
[2023-03-14 12:44:27,105] [INFO] Total running time: 0h0m50s