[2023-03-19 04:43:18,654] [INFO] DFAST_QC pipeline started.
[2023-03-19 04:43:18,654] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 04:43:18,655] [INFO] DQC Reference Directory: /var/lib/cwl/stgece6ee81-81bb-4ca6-8b2b-02a28d141aa3/dqc_reference
[2023-03-19 04:43:19,763] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 04:43:19,764] [INFO] Task started: Prodigal
[2023-03-19 04:43:19,764] [INFO] Running command: cat /var/lib/cwl/stg38bdd32a-5556-4667-b7f5-1a950c422c7f/OceanDNA-a3255.fa | prodigal -d OceanDNA-a3255/cds.fna -a OceanDNA-a3255/protein.faa -g 11 -q > /dev/null
[2023-03-19 04:43:27,525] [INFO] Task succeeded: Prodigal
[2023-03-19 04:43:27,525] [INFO] Task started: HMMsearch
[2023-03-19 04:43:27,525] [INFO] Running command: hmmsearch --tblout OceanDNA-a3255/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgece6ee81-81bb-4ca6-8b2b-02a28d141aa3/dqc_reference/reference_markers.hmm OceanDNA-a3255/protein.faa > /dev/null
[2023-03-19 04:43:27,659] [INFO] Task succeeded: HMMsearch
[2023-03-19 04:43:27,659] [WARNING] Found 2/6 markers. [/var/lib/cwl/stg38bdd32a-5556-4667-b7f5-1a950c422c7f/OceanDNA-a3255.fa]
[2023-03-19 04:43:27,668] [INFO] Query marker FASTA was written to OceanDNA-a3255/markers.fasta
[2023-03-19 04:43:27,668] [INFO] Task started: Blastn
[2023-03-19 04:43:27,668] [INFO] Running command: blastn -query OceanDNA-a3255/markers.fasta -db /var/lib/cwl/stgece6ee81-81bb-4ca6-8b2b-02a28d141aa3/dqc_reference/reference_markers.fasta -out OceanDNA-a3255/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 04:43:28,097] [INFO] Task succeeded: Blastn
[2023-03-19 04:43:28,098] [INFO] Selected 0 target genomes.
[2023-03-19 04:43:28,098] [INFO] Target genome list was writen to OceanDNA-a3255/target_genomes.txt
[2023-03-19 04:43:28,098] [ERROR] File is empty. [OceanDNA-a3255/target_genomes.txt]
[2023-03-19 04:43:28,098] [ERROR] Task failed. No target genome found.
[2023-03-19 04:43:28,098] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 04:43:28,098] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgece6ee81-81bb-4ca6-8b2b-02a28d141aa3/dqc_reference/checkm_data
[2023-03-19 04:43:28,151] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 04:43:28,154] [INFO] Task started: CheckM
[2023-03-19 04:43:28,154] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a3255/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a3255/checkm_input OceanDNA-a3255/checkm_result
[2023-03-19 04:43:56,313] [INFO] Task succeeded: CheckM
[2023-03-19 04:43:56,314] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 77.40%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 04:43:56,323] [INFO] ===== Completeness check finished =====
[2023-03-19 04:43:56,323] [INFO] ===== Start GTDB Search =====
[2023-03-19 04:43:56,323] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a3255/markers.fasta)
[2023-03-19 04:43:56,324] [INFO] Task started: Blastn
[2023-03-19 04:43:56,324] [INFO] Running command: blastn -query OceanDNA-a3255/markers.fasta -db /var/lib/cwl/stgece6ee81-81bb-4ca6-8b2b-02a28d141aa3/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a3255/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 04:43:56,794] [INFO] Task succeeded: Blastn
[2023-03-19 04:43:56,795] [INFO] Selected 2 target genomes.
[2023-03-19 04:43:56,795] [INFO] Target genome list was writen to OceanDNA-a3255/target_genomes_gtdb.txt
[2023-03-19 04:43:56,797] [INFO] Task started: fastANI
[2023-03-19 04:43:56,797] [INFO] Running command: fastANI --query /var/lib/cwl/stg38bdd32a-5556-4667-b7f5-1a950c422c7f/OceanDNA-a3255.fa --refList OceanDNA-a3255/target_genomes_gtdb.txt --output OceanDNA-a3255/fastani_result_gtdb.tsv --threads 1
[2023-03-19 04:43:57,471] [INFO] Task succeeded: fastANI
[2023-03-19 04:43:57,474] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-19 04:43:57,474] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002495675.1	s__MGIIb-N1 sp002495675	99.7641	360	401	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-N1	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002507175.1	s__MGIIb-N1 sp002507175	89.4446	354	401	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-N1	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-19 04:43:57,475] [INFO] GTDB search result was written to OceanDNA-a3255/result_gtdb.tsv
[2023-03-19 04:43:57,475] [INFO] ===== GTDB Search completed =====
[2023-03-19 04:43:57,475] [INFO] DFAST_QC result json was written to OceanDNA-a3255/dqc_result.json
[2023-03-19 04:43:57,475] [INFO] DFAST_QC completed!
[2023-03-19 04:43:57,475] [INFO] Total running time: 0h0m39s