[2023-03-19 00:01:03,331] [INFO] DFAST_QC pipeline started.
[2023-03-19 00:01:03,331] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 00:01:03,331] [INFO] DQC Reference Directory: /var/lib/cwl/stg85816b04-e80f-4d44-811d-1cf4deeba5b4/dqc_reference
[2023-03-19 00:01:04,638] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 00:01:04,638] [INFO] Task started: Prodigal
[2023-03-19 00:01:04,638] [INFO] Running command: cat /var/lib/cwl/stge908fac6-659f-442a-892e-0920ddd831c4/OceanDNA-a3763.fa | prodigal -d OceanDNA-a3763/cds.fna -a OceanDNA-a3763/protein.faa -g 11 -q > /dev/null
[2023-03-19 00:01:10,137] [INFO] Task succeeded: Prodigal
[2023-03-19 00:01:10,138] [INFO] Task started: HMMsearch
[2023-03-19 00:01:10,138] [INFO] Running command: hmmsearch --tblout OceanDNA-a3763/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg85816b04-e80f-4d44-811d-1cf4deeba5b4/dqc_reference/reference_markers.hmm OceanDNA-a3763/protein.faa > /dev/null
[2023-03-19 00:01:10,329] [INFO] Task succeeded: HMMsearch
[2023-03-19 00:01:10,329] [WARNING] Found 2/6 markers. [/var/lib/cwl/stge908fac6-659f-442a-892e-0920ddd831c4/OceanDNA-a3763.fa]
[2023-03-19 00:01:10,339] [INFO] Query marker FASTA was written to OceanDNA-a3763/markers.fasta
[2023-03-19 00:01:10,340] [INFO] Task started: Blastn
[2023-03-19 00:01:10,340] [INFO] Running command: blastn -query OceanDNA-a3763/markers.fasta -db /var/lib/cwl/stg85816b04-e80f-4d44-811d-1cf4deeba5b4/dqc_reference/reference_markers.fasta -out OceanDNA-a3763/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 00:01:10,815] [INFO] Task succeeded: Blastn
[2023-03-19 00:01:10,815] [INFO] Selected 5 target genomes.
[2023-03-19 00:01:10,816] [INFO] Target genome list was writen to OceanDNA-a3763/target_genomes.txt
[2023-03-19 00:01:10,819] [INFO] Task started: fastANI
[2023-03-19 00:01:10,819] [INFO] Running command: fastANI --query /var/lib/cwl/stge908fac6-659f-442a-892e-0920ddd831c4/OceanDNA-a3763.fa --refList OceanDNA-a3763/target_genomes.txt --output OceanDNA-a3763/fastani_result.tsv --threads 1
[2023-03-19 00:01:13,399] [INFO] Task succeeded: fastANI
[2023-03-19 00:01:13,400] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg85816b04-e80f-4d44-811d-1cf4deeba5b4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 00:01:13,400] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg85816b04-e80f-4d44-811d-1cf4deeba5b4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 00:01:13,400] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 00:01:13,400] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-19 00:01:13,400] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-19 00:01:13,400] [INFO] DFAST Taxonomy check result was written to OceanDNA-a3763/tc_result.tsv
[2023-03-19 00:01:13,400] [INFO] ===== Taxonomy check completed =====
[2023-03-19 00:01:13,401] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 00:01:13,401] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg85816b04-e80f-4d44-811d-1cf4deeba5b4/dqc_reference/checkm_data
[2023-03-19 00:01:13,453] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 00:01:13,538] [INFO] Task started: CheckM
[2023-03-19 00:01:13,538] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a3763/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a3763/checkm_input OceanDNA-a3763/checkm_result
[2023-03-19 00:01:34,211] [INFO] Task succeeded: CheckM
[2023-03-19 00:01:34,211] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 62.63%
Contamintation: 0.46%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 00:01:34,213] [INFO] ===== Completeness check finished =====
[2023-03-19 00:01:34,213] [INFO] ===== Start GTDB Search =====
[2023-03-19 00:01:34,213] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a3763/markers.fasta)
[2023-03-19 00:01:34,215] [INFO] Task started: Blastn
[2023-03-19 00:01:34,215] [INFO] Running command: blastn -query OceanDNA-a3763/markers.fasta -db /var/lib/cwl/stg85816b04-e80f-4d44-811d-1cf4deeba5b4/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a3763/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 00:01:34,966] [INFO] Task succeeded: Blastn
[2023-03-19 00:01:34,967] [INFO] Selected 7 target genomes.
[2023-03-19 00:01:34,967] [INFO] Target genome list was writen to OceanDNA-a3763/target_genomes_gtdb.txt
[2023-03-19 00:01:34,979] [INFO] Task started: fastANI
[2023-03-19 00:01:34,979] [INFO] Running command: fastANI --query /var/lib/cwl/stge908fac6-659f-442a-892e-0920ddd831c4/OceanDNA-a3763.fa --refList OceanDNA-a3763/target_genomes_gtdb.txt --output OceanDNA-a3763/fastani_result_gtdb.tsv --threads 1
[2023-03-19 00:01:36,999] [INFO] Task succeeded: fastANI
[2023-03-19 00:01:37,004] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-19 00:01:37,004] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_902593945.1	s__MGIIb-O2 sp902593945	98.6749	260	343	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002497245.1	s__MGIIb-O2 sp002497245	88.5796	303	343	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	97.50	97.50	0.90	0.90	2	-
GCA_002504905.1	s__MGIIb-O2 sp002504905	83.5168	291	343	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	99.85	99.68	0.95	0.91	6	-
GCA_002495525.1	s__MGIIb-O2 sp002495525	83.083	271	343	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	98.21	96.68	0.79	0.75	3	-
GCA_002498985.1	s__MGIIb-O2 sp002498985	82.6372	248	343	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	97.30	95.20	0.78	0.62	4	-
GCA_002685315.1	s__MGIIb-O2 sp002685315	82.3539	189	343	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002686525.1	s__MGIIb-O2 sp002686525	79.4833	222	343	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O2	95.0	97.02	96.68	0.82	0.74	10	-
--------------------------------------------------------------------------------
[2023-03-19 00:01:37,004] [INFO] GTDB search result was written to OceanDNA-a3763/result_gtdb.tsv
[2023-03-19 00:01:37,004] [INFO] ===== GTDB Search completed =====
[2023-03-19 00:01:37,005] [INFO] DFAST_QC result json was written to OceanDNA-a3763/dqc_result.json
[2023-03-19 00:01:37,005] [INFO] DFAST_QC completed!
[2023-03-19 00:01:37,005] [INFO] Total running time: 0h0m34s