[2023-03-17 03:29:19,768] [INFO] DFAST_QC pipeline started.
[2023-03-17 03:29:19,769] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 03:29:19,769] [INFO] DQC Reference Directory: /var/lib/cwl/stga0ffdaf7-86b3-422d-88d5-fe8d14419380/dqc_reference
[2023-03-17 03:29:21,321] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 03:29:21,321] [INFO] Task started: Prodigal
[2023-03-17 03:29:21,322] [INFO] Running command: cat /var/lib/cwl/stg59b3756b-3465-4db4-ac59-361db921129c/OceanDNA-a4102.fa | prodigal -d OceanDNA-a4102/cds.fna -a OceanDNA-a4102/protein.faa -g 11 -q > /dev/null
[2023-03-17 03:29:29,594] [INFO] Task succeeded: Prodigal
[2023-03-17 03:29:29,595] [INFO] Task started: HMMsearch
[2023-03-17 03:29:29,595] [INFO] Running command: hmmsearch --tblout OceanDNA-a4102/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga0ffdaf7-86b3-422d-88d5-fe8d14419380/dqc_reference/reference_markers.hmm OceanDNA-a4102/protein.faa > /dev/null
[2023-03-17 03:29:29,750] [INFO] Task succeeded: HMMsearch
[2023-03-17 03:29:29,750] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg59b3756b-3465-4db4-ac59-361db921129c/OceanDNA-a4102.fa]
[2023-03-17 03:29:29,760] [INFO] Query marker FASTA was written to OceanDNA-a4102/markers.fasta
[2023-03-17 03:29:29,760] [INFO] Task started: Blastn
[2023-03-17 03:29:29,760] [INFO] Running command: blastn -query OceanDNA-a4102/markers.fasta -db /var/lib/cwl/stga0ffdaf7-86b3-422d-88d5-fe8d14419380/dqc_reference/reference_markers.fasta -out OceanDNA-a4102/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 03:29:30,178] [INFO] Task succeeded: Blastn
[2023-03-17 03:29:30,178] [INFO] Selected 5 target genomes.
[2023-03-17 03:29:30,178] [INFO] Target genome list was writen to OceanDNA-a4102/target_genomes.txt
[2023-03-17 03:29:30,181] [INFO] Task started: fastANI
[2023-03-17 03:29:30,181] [INFO] Running command: fastANI --query /var/lib/cwl/stg59b3756b-3465-4db4-ac59-361db921129c/OceanDNA-a4102.fa --refList OceanDNA-a4102/target_genomes.txt --output OceanDNA-a4102/fastani_result.tsv --threads 1
[2023-03-17 03:29:32,664] [INFO] Task succeeded: fastANI
[2023-03-17 03:29:32,664] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga0ffdaf7-86b3-422d-88d5-fe8d14419380/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 03:29:32,665] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga0ffdaf7-86b3-422d-88d5-fe8d14419380/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 03:29:32,665] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 03:29:32,665] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 03:29:32,665] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 03:29:32,665] [INFO] DFAST Taxonomy check result was written to OceanDNA-a4102/tc_result.tsv
[2023-03-17 03:29:32,665] [INFO] ===== Taxonomy check completed =====
[2023-03-17 03:29:32,665] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 03:29:32,665] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga0ffdaf7-86b3-422d-88d5-fe8d14419380/dqc_reference/checkm_data
[2023-03-17 03:29:32,668] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 03:29:32,671] [INFO] Task started: CheckM
[2023-03-17 03:29:32,671] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a4102/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a4102/checkm_input OceanDNA-a4102/checkm_result
[2023-03-17 03:30:00,211] [INFO] Task succeeded: CheckM
[2023-03-17 03:30:00,211] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 94.07%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-17 03:30:00,213] [INFO] ===== Completeness check finished =====
[2023-03-17 03:30:00,213] [INFO] ===== Start GTDB Search =====
[2023-03-17 03:30:00,213] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a4102/markers.fasta)
[2023-03-17 03:30:00,213] [INFO] Task started: Blastn
[2023-03-17 03:30:00,214] [INFO] Running command: blastn -query OceanDNA-a4102/markers.fasta -db /var/lib/cwl/stga0ffdaf7-86b3-422d-88d5-fe8d14419380/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a4102/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 03:30:00,626] [INFO] Task succeeded: Blastn
[2023-03-17 03:30:00,627] [INFO] Selected 10 target genomes.
[2023-03-17 03:30:00,627] [INFO] Target genome list was writen to OceanDNA-a4102/target_genomes_gtdb.txt
[2023-03-17 03:30:00,638] [INFO] Task started: fastANI
[2023-03-17 03:30:00,638] [INFO] Running command: fastANI --query /var/lib/cwl/stg59b3756b-3465-4db4-ac59-361db921129c/OceanDNA-a4102.fa --refList OceanDNA-a4102/target_genomes_gtdb.txt --output OceanDNA-a4102/fastani_result_gtdb.tsv --threads 1
[2023-03-17 03:30:03,947] [INFO] Task succeeded: fastANI
[2023-03-17 03:30:03,951] [INFO] Found 5 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-17 03:30:03,952] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002725315.1	s__MGIIb-O3 sp002725315	80.1029	252	452	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O3	95.0	99.12	98.81	0.90	0.86	5	-
GCA_001628485.1	s__MGIIb-O3 sp001628485	77.542	84	452	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O3	95.0	98.15	97.12	0.88	0.79	10	-
GCA_902601345.1	s__MGIIb-O3 sp902601345	77.2658	58	452	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O3	95.0	N/A	N/A	N/A	N/A	1	-
GCA_001629255.1	s__MGIIb-O3 sp001629255	77.0541	62	452	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O3	95.0	98.42	97.36	0.80	0.70	6	-
GCA_001629235.1	s__MGIIb-O3 sp001629235	76.6664	71	452	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O3	95.0	97.45	95.03	0.80	0.68	7	-
--------------------------------------------------------------------------------
[2023-03-17 03:30:03,952] [INFO] GTDB search result was written to OceanDNA-a4102/result_gtdb.tsv
[2023-03-17 03:30:03,952] [INFO] ===== GTDB Search completed =====
[2023-03-17 03:30:03,952] [INFO] DFAST_QC result json was written to OceanDNA-a4102/dqc_result.json
[2023-03-17 03:30:03,953] [INFO] DFAST_QC completed!
[2023-03-17 03:30:03,953] [INFO] Total running time: 0h0m44s