[2023-03-18 05:05:08,004] [INFO] DFAST_QC pipeline started.
[2023-03-18 05:05:08,009] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 05:05:08,009] [INFO] DQC Reference Directory: /var/lib/cwl/stg3af4a868-1552-471a-be3a-fac58758e19d/dqc_reference
[2023-03-18 05:05:09,104] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 05:05:09,104] [INFO] Task started: Prodigal
[2023-03-18 05:05:09,104] [INFO] Running command: cat /var/lib/cwl/stg8c0641fc-4fda-4e33-a041-e5bd42d02455/OceanDNA-a4214.fa | prodigal -d OceanDNA-a4214/cds.fna -a OceanDNA-a4214/protein.faa -g 11 -q > /dev/null
[2023-03-18 05:05:15,278] [INFO] Task succeeded: Prodigal
[2023-03-18 05:05:15,278] [INFO] Task started: HMMsearch
[2023-03-18 05:05:15,278] [INFO] Running command: hmmsearch --tblout OceanDNA-a4214/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3af4a868-1552-471a-be3a-fac58758e19d/dqc_reference/reference_markers.hmm OceanDNA-a4214/protein.faa > /dev/null
[2023-03-18 05:05:15,429] [INFO] Task succeeded: HMMsearch
[2023-03-18 05:05:15,430] [WARNING] Found 2/6 markers. [/var/lib/cwl/stg8c0641fc-4fda-4e33-a041-e5bd42d02455/OceanDNA-a4214.fa]
[2023-03-18 05:05:15,468] [INFO] Query marker FASTA was written to OceanDNA-a4214/markers.fasta
[2023-03-18 05:05:15,469] [INFO] Task started: Blastn
[2023-03-18 05:05:15,469] [INFO] Running command: blastn -query OceanDNA-a4214/markers.fasta -db /var/lib/cwl/stg3af4a868-1552-471a-be3a-fac58758e19d/dqc_reference/reference_markers.fasta -out OceanDNA-a4214/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 05:05:15,932] [INFO] Task succeeded: Blastn
[2023-03-18 05:05:15,947] [INFO] Selected 3 target genomes.
[2023-03-18 05:05:15,948] [INFO] Target genome list was writen to OceanDNA-a4214/target_genomes.txt
[2023-03-18 05:05:15,950] [INFO] Task started: fastANI
[2023-03-18 05:05:15,950] [INFO] Running command: fastANI --query /var/lib/cwl/stg8c0641fc-4fda-4e33-a041-e5bd42d02455/OceanDNA-a4214.fa --refList OceanDNA-a4214/target_genomes.txt --output OceanDNA-a4214/fastani_result.tsv --threads 1
[2023-03-18 05:05:17,166] [INFO] Task succeeded: fastANI
[2023-03-18 05:05:17,166] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3af4a868-1552-471a-be3a-fac58758e19d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 05:05:17,166] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3af4a868-1552-471a-be3a-fac58758e19d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 05:05:17,167] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 05:05:17,167] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 05:05:17,167] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 05:05:17,169] [INFO] DFAST Taxonomy check result was written to OceanDNA-a4214/tc_result.tsv
[2023-03-18 05:05:17,170] [INFO] ===== Taxonomy check completed =====
[2023-03-18 05:05:17,170] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 05:05:17,170] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3af4a868-1552-471a-be3a-fac58758e19d/dqc_reference/checkm_data
[2023-03-18 05:05:17,173] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 05:05:17,207] [INFO] Task started: CheckM
[2023-03-18 05:05:17,207] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a4214/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a4214/checkm_input OceanDNA-a4214/checkm_result
[2023-03-18 05:05:36,482] [INFO] Task succeeded: CheckM
[2023-03-18 05:05:36,482] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 61.96%
Contamintation: 0.46%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 05:05:36,508] [INFO] ===== Completeness check finished =====
[2023-03-18 05:05:36,508] [INFO] ===== Start GTDB Search =====
[2023-03-18 05:05:36,508] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a4214/markers.fasta)
[2023-03-18 05:05:36,509] [INFO] Task started: Blastn
[2023-03-18 05:05:36,509] [INFO] Running command: blastn -query OceanDNA-a4214/markers.fasta -db /var/lib/cwl/stg3af4a868-1552-471a-be3a-fac58758e19d/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a4214/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 05:05:37,046] [INFO] Task succeeded: Blastn
[2023-03-18 05:05:37,060] [INFO] Selected 6 target genomes.
[2023-03-18 05:05:37,060] [INFO] Target genome list was writen to OceanDNA-a4214/target_genomes_gtdb.txt
[2023-03-18 05:05:37,154] [INFO] Task started: fastANI
[2023-03-18 05:05:37,154] [INFO] Running command: fastANI --query /var/lib/cwl/stg8c0641fc-4fda-4e33-a041-e5bd42d02455/OceanDNA-a4214.fa --refList OceanDNA-a4214/target_genomes_gtdb.txt --output OceanDNA-a4214/fastani_result_gtdb.tsv --threads 1
[2023-03-18 05:05:38,516] [INFO] Task succeeded: fastANI
[2023-03-18 05:05:38,519] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 05:05:38,519] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_004195615.1	s__MGIIb-O3 sp004195615	80.9165	194	241	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O3	95.0	99.94	99.91	0.95	0.93	3	-
GCA_014239955.1	s__MGIIb-O3 sp014239955	80.8896	185	241	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-O3	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 05:05:38,524] [INFO] GTDB search result was written to OceanDNA-a4214/result_gtdb.tsv
[2023-03-18 05:05:38,537] [INFO] ===== GTDB Search completed =====
[2023-03-18 05:05:38,543] [INFO] DFAST_QC result json was written to OceanDNA-a4214/dqc_result.json
[2023-03-18 05:05:38,543] [INFO] DFAST_QC completed!
[2023-03-18 05:05:38,543] [INFO] Total running time: 0h0m31s