[2023-03-16 04:05:20,020] [INFO] DFAST_QC pipeline started.
[2023-03-16 04:05:20,020] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 04:05:20,020] [INFO] DQC Reference Directory: /var/lib/cwl/stgfbbbf968-07bf-4dc9-bdfc-69a26e7d6476/dqc_reference
[2023-03-16 04:05:21,130] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 04:05:21,131] [INFO] Task started: Prodigal
[2023-03-16 04:05:21,131] [INFO] Running command: cat /var/lib/cwl/stg9d3bfb28-f94b-4dc4-abb8-185c4281fd76/OceanDNA-a4760.fa | prodigal -d OceanDNA-a4760/cds.fna -a OceanDNA-a4760/protein.faa -g 11 -q > /dev/null
[2023-03-16 04:05:25,468] [INFO] Task succeeded: Prodigal
[2023-03-16 04:05:25,468] [INFO] Task started: HMMsearch
[2023-03-16 04:05:25,468] [INFO] Running command: hmmsearch --tblout OceanDNA-a4760/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfbbbf968-07bf-4dc9-bdfc-69a26e7d6476/dqc_reference/reference_markers.hmm OceanDNA-a4760/protein.faa > /dev/null
[2023-03-16 04:05:25,586] [INFO] Task succeeded: HMMsearch
[2023-03-16 04:05:25,586] [WARNING] Found 1/6 markers. [/var/lib/cwl/stg9d3bfb28-f94b-4dc4-abb8-185c4281fd76/OceanDNA-a4760.fa]
[2023-03-16 04:05:25,594] [INFO] Query marker FASTA was written to OceanDNA-a4760/markers.fasta
[2023-03-16 04:05:25,594] [INFO] Task started: Blastn
[2023-03-16 04:05:25,594] [INFO] Running command: blastn -query OceanDNA-a4760/markers.fasta -db /var/lib/cwl/stgfbbbf968-07bf-4dc9-bdfc-69a26e7d6476/dqc_reference/reference_markers.fasta -out OceanDNA-a4760/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 04:05:26,005] [INFO] Task succeeded: Blastn
[2023-03-16 04:05:26,006] [INFO] Selected 0 target genomes.
[2023-03-16 04:05:26,006] [INFO] Target genome list was writen to OceanDNA-a4760/target_genomes.txt
[2023-03-16 04:05:26,006] [ERROR] File is empty. [OceanDNA-a4760/target_genomes.txt]
[2023-03-16 04:05:26,006] [ERROR] Task failed. No target genome found.
[2023-03-16 04:05:26,006] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 04:05:26,006] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfbbbf968-07bf-4dc9-bdfc-69a26e7d6476/dqc_reference/checkm_data
[2023-03-16 04:05:26,060] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 04:05:26,062] [INFO] Task started: CheckM
[2023-03-16 04:05:26,062] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a4760/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a4760/checkm_input OceanDNA-a4760/checkm_result
[2023-03-16 04:05:44,156] [INFO] Task succeeded: CheckM
[2023-03-16 04:05:44,156] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 43.17%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 04:05:44,158] [INFO] ===== Completeness check finished =====
[2023-03-16 04:05:44,158] [INFO] ===== Start GTDB Search =====
[2023-03-16 04:05:44,158] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a4760/markers.fasta)
[2023-03-16 04:05:44,158] [INFO] Task started: Blastn
[2023-03-16 04:05:44,158] [INFO] Running command: blastn -query OceanDNA-a4760/markers.fasta -db /var/lib/cwl/stgfbbbf968-07bf-4dc9-bdfc-69a26e7d6476/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a4760/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 04:05:44,871] [INFO] Task succeeded: Blastn
[2023-03-16 04:05:44,871] [INFO] Selected 3 target genomes.
[2023-03-16 04:05:44,871] [INFO] Target genome list was writen to OceanDNA-a4760/target_genomes_gtdb.txt
[2023-03-16 04:05:44,887] [INFO] Task started: fastANI
[2023-03-16 04:05:44,887] [INFO] Running command: fastANI --query /var/lib/cwl/stg9d3bfb28-f94b-4dc4-abb8-185c4281fd76/OceanDNA-a4760.fa --refList OceanDNA-a4760/target_genomes_gtdb.txt --output OceanDNA-a4760/fastani_result_gtdb.tsv --threads 1
[2023-03-16 04:05:45,616] [INFO] Task succeeded: fastANI
[2023-03-16 04:05:45,617] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 04:05:45,618] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002498455.1	s__MGIIb-P sp002498455	79.4769	150	218	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-P	95.0	98.13	97.46	0.87	0.81	6	-
--------------------------------------------------------------------------------
[2023-03-16 04:05:45,618] [INFO] GTDB search result was written to OceanDNA-a4760/result_gtdb.tsv
[2023-03-16 04:05:45,618] [INFO] ===== GTDB Search completed =====
[2023-03-16 04:05:45,618] [INFO] DFAST_QC result json was written to OceanDNA-a4760/dqc_result.json
[2023-03-16 04:05:45,618] [INFO] DFAST_QC completed!
[2023-03-16 04:05:45,618] [INFO] Total running time: 0h0m26s