[2023-03-17 23:10:23,653] [INFO] DFAST_QC pipeline started.
[2023-03-17 23:10:23,653] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 23:10:23,654] [INFO] DQC Reference Directory: /var/lib/cwl/stg4bbb4f0a-dbab-4d19-98fc-35b40881f00f/dqc_reference
[2023-03-17 23:10:24,742] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 23:10:24,742] [INFO] Task started: Prodigal
[2023-03-17 23:10:24,742] [INFO] Running command: cat /var/lib/cwl/stgff2e73c6-4b2b-4415-8613-21b36a5072a8/OceanDNA-a4770.fa | prodigal -d OceanDNA-a4770/cds.fna -a OceanDNA-a4770/protein.faa -g 11 -q > /dev/null
[2023-03-17 23:10:32,559] [INFO] Task succeeded: Prodigal
[2023-03-17 23:10:32,560] [INFO] Task started: HMMsearch
[2023-03-17 23:10:32,560] [INFO] Running command: hmmsearch --tblout OceanDNA-a4770/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg4bbb4f0a-dbab-4d19-98fc-35b40881f00f/dqc_reference/reference_markers.hmm OceanDNA-a4770/protein.faa > /dev/null
[2023-03-17 23:10:32,716] [INFO] Task succeeded: HMMsearch
[2023-03-17 23:10:32,716] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgff2e73c6-4b2b-4415-8613-21b36a5072a8/OceanDNA-a4770.fa]
[2023-03-17 23:10:32,729] [INFO] Query marker FASTA was written to OceanDNA-a4770/markers.fasta
[2023-03-17 23:10:32,730] [INFO] Task started: Blastn
[2023-03-17 23:10:32,730] [INFO] Running command: blastn -query OceanDNA-a4770/markers.fasta -db /var/lib/cwl/stg4bbb4f0a-dbab-4d19-98fc-35b40881f00f/dqc_reference/reference_markers.fasta -out OceanDNA-a4770/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 23:10:33,154] [INFO] Task succeeded: Blastn
[2023-03-17 23:10:33,155] [INFO] Selected 5 target genomes.
[2023-03-17 23:10:33,155] [INFO] Target genome list was writen to OceanDNA-a4770/target_genomes.txt
[2023-03-17 23:10:33,159] [INFO] Task started: fastANI
[2023-03-17 23:10:33,159] [INFO] Running command: fastANI --query /var/lib/cwl/stgff2e73c6-4b2b-4415-8613-21b36a5072a8/OceanDNA-a4770.fa --refList OceanDNA-a4770/target_genomes.txt --output OceanDNA-a4770/fastani_result.tsv --threads 1
[2023-03-17 23:10:35,772] [INFO] Task succeeded: fastANI
[2023-03-17 23:10:35,772] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg4bbb4f0a-dbab-4d19-98fc-35b40881f00f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 23:10:35,772] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg4bbb4f0a-dbab-4d19-98fc-35b40881f00f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 23:10:35,773] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 23:10:35,773] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 23:10:35,773] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 23:10:35,774] [INFO] DFAST Taxonomy check result was written to OceanDNA-a4770/tc_result.tsv
[2023-03-17 23:10:35,774] [INFO] ===== Taxonomy check completed =====
[2023-03-17 23:10:35,774] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 23:10:35,774] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg4bbb4f0a-dbab-4d19-98fc-35b40881f00f/dqc_reference/checkm_data
[2023-03-17 23:10:35,777] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 23:10:35,780] [INFO] Task started: CheckM
[2023-03-17 23:10:35,780] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a4770/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a4770/checkm_input OceanDNA-a4770/checkm_result
[2023-03-17 23:11:01,057] [INFO] Task succeeded: CheckM
[2023-03-17 23:11:01,057] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 70.20%
Contamintation: 1.50%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-17 23:11:01,065] [INFO] ===== Completeness check finished =====
[2023-03-17 23:11:01,065] [INFO] ===== Start GTDB Search =====
[2023-03-17 23:11:01,065] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a4770/markers.fasta)
[2023-03-17 23:11:01,066] [INFO] Task started: Blastn
[2023-03-17 23:11:01,066] [INFO] Running command: blastn -query OceanDNA-a4770/markers.fasta -db /var/lib/cwl/stg4bbb4f0a-dbab-4d19-98fc-35b40881f00f/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a4770/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 23:11:01,463] [INFO] Task succeeded: Blastn
[2023-03-17 23:11:01,466] [INFO] Selected 6 target genomes.
[2023-03-17 23:11:01,466] [INFO] Target genome list was writen to OceanDNA-a4770/target_genomes_gtdb.txt
[2023-03-17 23:11:01,469] [INFO] Task started: fastANI
[2023-03-17 23:11:01,469] [INFO] Running command: fastANI --query /var/lib/cwl/stgff2e73c6-4b2b-4415-8613-21b36a5072a8/OceanDNA-a4770.fa --refList OceanDNA-a4770/target_genomes_gtdb.txt --output OceanDNA-a4770/fastani_result_gtdb.tsv --threads 1
[2023-03-17 23:11:02,999] [INFO] Task succeeded: fastANI
[2023-03-17 23:11:02,999] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-17 23:11:02,999] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-17 23:11:03,002] [INFO] GTDB search result was written to OceanDNA-a4770/result_gtdb.tsv
[2023-03-17 23:11:03,003] [INFO] ===== GTDB Search completed =====
[2023-03-17 23:11:03,005] [INFO] DFAST_QC result json was written to OceanDNA-a4770/dqc_result.json
[2023-03-17 23:11:03,005] [INFO] DFAST_QC completed!
[2023-03-17 23:11:03,005] [INFO] Total running time: 0h0m39s