[2023-03-15 10:21:47,932] [INFO] DFAST_QC pipeline started.
[2023-03-15 10:21:47,932] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 10:21:47,932] [INFO] DQC Reference Directory: /var/lib/cwl/stgc3ac4a09-a4a0-4e98-bcd5-81943021c6e5/dqc_reference
[2023-03-15 10:21:49,124] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 10:21:49,124] [INFO] Task started: Prodigal
[2023-03-15 10:21:49,125] [INFO] Running command: cat /var/lib/cwl/stg286cfc52-ea77-46a9-ac75-2f78b0bd023c/OceanDNA-a4788.fa | prodigal -d OceanDNA-a4788/cds.fna -a OceanDNA-a4788/protein.faa -g 11 -q > /dev/null
[2023-03-15 10:21:58,777] [INFO] Task succeeded: Prodigal
[2023-03-15 10:21:58,777] [INFO] Task started: HMMsearch
[2023-03-15 10:21:58,778] [INFO] Running command: hmmsearch --tblout OceanDNA-a4788/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc3ac4a09-a4a0-4e98-bcd5-81943021c6e5/dqc_reference/reference_markers.hmm OceanDNA-a4788/protein.faa > /dev/null
[2023-03-15 10:21:58,954] [INFO] Task succeeded: HMMsearch
[2023-03-15 10:21:58,955] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg286cfc52-ea77-46a9-ac75-2f78b0bd023c/OceanDNA-a4788.fa]
[2023-03-15 10:21:58,967] [INFO] Query marker FASTA was written to OceanDNA-a4788/markers.fasta
[2023-03-15 10:21:58,967] [INFO] Task started: Blastn
[2023-03-15 10:21:58,967] [INFO] Running command: blastn -query OceanDNA-a4788/markers.fasta -db /var/lib/cwl/stgc3ac4a09-a4a0-4e98-bcd5-81943021c6e5/dqc_reference/reference_markers.fasta -out OceanDNA-a4788/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 10:21:59,393] [INFO] Task succeeded: Blastn
[2023-03-15 10:21:59,394] [INFO] Selected 1 target genomes.
[2023-03-15 10:21:59,394] [INFO] Target genome list was writen to OceanDNA-a4788/target_genomes.txt
[2023-03-15 10:21:59,395] [INFO] Task started: fastANI
[2023-03-15 10:21:59,395] [INFO] Running command: fastANI --query /var/lib/cwl/stg286cfc52-ea77-46a9-ac75-2f78b0bd023c/OceanDNA-a4788.fa --refList OceanDNA-a4788/target_genomes.txt --output OceanDNA-a4788/fastani_result.tsv --threads 1
[2023-03-15 10:21:59,807] [INFO] Task succeeded: fastANI
[2023-03-15 10:21:59,808] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc3ac4a09-a4a0-4e98-bcd5-81943021c6e5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 10:21:59,808] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc3ac4a09-a4a0-4e98-bcd5-81943021c6e5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 10:21:59,808] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 10:21:59,808] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 10:21:59,808] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 10:21:59,809] [INFO] DFAST Taxonomy check result was written to OceanDNA-a4788/tc_result.tsv
[2023-03-15 10:21:59,809] [INFO] ===== Taxonomy check completed =====
[2023-03-15 10:21:59,809] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 10:21:59,809] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc3ac4a09-a4a0-4e98-bcd5-81943021c6e5/dqc_reference/checkm_data
[2023-03-15 10:21:59,811] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 10:21:59,814] [INFO] Task started: CheckM
[2023-03-15 10:21:59,814] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a4788/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a4788/checkm_input OceanDNA-a4788/checkm_result
[2023-03-15 10:22:29,557] [INFO] Task succeeded: CheckM
[2023-03-15 10:22:29,558] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 80.24%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 10:22:29,559] [INFO] ===== Completeness check finished =====
[2023-03-15 10:22:29,559] [INFO] ===== Start GTDB Search =====
[2023-03-15 10:22:29,560] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a4788/markers.fasta)
[2023-03-15 10:22:29,560] [INFO] Task started: Blastn
[2023-03-15 10:22:29,560] [INFO] Running command: blastn -query OceanDNA-a4788/markers.fasta -db /var/lib/cwl/stgc3ac4a09-a4a0-4e98-bcd5-81943021c6e5/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a4788/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 10:22:29,983] [INFO] Task succeeded: Blastn
[2023-03-15 10:22:29,984] [INFO] Selected 4 target genomes.
[2023-03-15 10:22:29,984] [INFO] Target genome list was writen to OceanDNA-a4788/target_genomes_gtdb.txt
[2023-03-15 10:22:30,058] [INFO] Task started: fastANI
[2023-03-15 10:22:30,058] [INFO] Running command: fastANI --query /var/lib/cwl/stg286cfc52-ea77-46a9-ac75-2f78b0bd023c/OceanDNA-a4788.fa --refList OceanDNA-a4788/target_genomes_gtdb.txt --output OceanDNA-a4788/fastani_result_gtdb.tsv --threads 1
[2023-03-15 10:22:31,386] [INFO] Task succeeded: fastANI
[2023-03-15 10:22:31,390] [INFO] Found 4 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 10:22:31,390] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002505685.1	s__MGIIb-P sp002505685	99.3684	375	494	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-P	95.0	99.68	99.68	0.73	0.73	2	conclusive
GCA_002724815.1	s__MGIIb-P sp002724815	79.0132	252	494	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-P	95.0	96.66	96.66	0.89	0.89	2	-
GCA_002498455.1	s__MGIIb-P sp002498455	78.8559	251	494	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-P	95.0	98.13	97.46	0.87	0.81	6	-
GCA_002701965.1	s__MGIIb-P sp002701965	78.7009	197	494	d__Archaea;p__Thermoplasmatota;c__Poseidoniia;o__Poseidoniales;f__Thalassarchaeaceae;g__MGIIb-P	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 10:22:31,390] [INFO] GTDB search result was written to OceanDNA-a4788/result_gtdb.tsv
[2023-03-15 10:22:31,390] [INFO] ===== GTDB Search completed =====
[2023-03-15 10:22:31,391] [INFO] DFAST_QC result json was written to OceanDNA-a4788/dqc_result.json
[2023-03-15 10:22:31,391] [INFO] DFAST_QC completed!
[2023-03-15 10:22:31,391] [INFO] Total running time: 0h0m43s