[2023-03-19 05:32:20,809] [INFO] DFAST_QC pipeline started.
[2023-03-19 05:32:20,809] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 05:32:20,809] [INFO] DQC Reference Directory: /var/lib/cwl/stga9574de0-4a7d-4741-9a8a-eefa1f5dcd30/dqc_reference
[2023-03-19 05:32:22,378] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 05:32:22,379] [INFO] Task started: Prodigal
[2023-03-19 05:32:22,379] [INFO] Running command: cat /var/lib/cwl/stg65244fb5-7e01-403e-9620-0c0383e1d720/OceanDNA-a97.fa | prodigal -d OceanDNA-a97/cds.fna -a OceanDNA-a97/protein.faa -g 11 -q > /dev/null
[2023-03-19 05:32:26,376] [INFO] Task succeeded: Prodigal
[2023-03-19 05:32:26,376] [INFO] Task started: HMMsearch
[2023-03-19 05:32:26,376] [INFO] Running command: hmmsearch --tblout OceanDNA-a97/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga9574de0-4a7d-4741-9a8a-eefa1f5dcd30/dqc_reference/reference_markers.hmm OceanDNA-a97/protein.faa > /dev/null
[2023-03-19 05:32:26,483] [INFO] Task succeeded: HMMsearch
[2023-03-19 05:32:26,483] [WARNING] Found 1/6 markers. [/var/lib/cwl/stg65244fb5-7e01-403e-9620-0c0383e1d720/OceanDNA-a97.fa]
[2023-03-19 05:32:26,491] [INFO] Query marker FASTA was written to OceanDNA-a97/markers.fasta
[2023-03-19 05:32:26,491] [INFO] Task started: Blastn
[2023-03-19 05:32:26,492] [INFO] Running command: blastn -query OceanDNA-a97/markers.fasta -db /var/lib/cwl/stga9574de0-4a7d-4741-9a8a-eefa1f5dcd30/dqc_reference/reference_markers.fasta -out OceanDNA-a97/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 05:32:28,015] [INFO] Task succeeded: Blastn
[2023-03-19 05:32:28,015] [INFO] Selected 3 target genomes.
[2023-03-19 05:32:28,016] [INFO] Target genome list was writen to OceanDNA-a97/target_genomes.txt
[2023-03-19 05:32:28,017] [INFO] Task started: fastANI
[2023-03-19 05:32:28,017] [INFO] Running command: fastANI --query /var/lib/cwl/stg65244fb5-7e01-403e-9620-0c0383e1d720/OceanDNA-a97.fa --refList OceanDNA-a97/target_genomes.txt --output OceanDNA-a97/fastani_result.tsv --threads 1
[2023-03-19 05:32:28,823] [INFO] Task succeeded: fastANI
[2023-03-19 05:32:28,824] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga9574de0-4a7d-4741-9a8a-eefa1f5dcd30/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 05:32:28,824] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga9574de0-4a7d-4741-9a8a-eefa1f5dcd30/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 05:32:28,824] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 05:32:28,824] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-19 05:32:28,824] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-19 05:32:28,824] [INFO] DFAST Taxonomy check result was written to OceanDNA-a97/tc_result.tsv
[2023-03-19 05:32:28,824] [INFO] ===== Taxonomy check completed =====
[2023-03-19 05:32:28,824] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 05:32:28,825] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga9574de0-4a7d-4741-9a8a-eefa1f5dcd30/dqc_reference/checkm_data
[2023-03-19 05:32:28,827] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 05:32:28,829] [INFO] Task started: CheckM
[2023-03-19 05:32:28,829] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-a97/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-a97/checkm_input OceanDNA-a97/checkm_result
[2023-03-19 05:32:45,065] [INFO] Task succeeded: CheckM
[2023-03-19 05:32:45,066] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.95%
Contamintation: 6.71%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 05:32:45,067] [INFO] ===== Completeness check finished =====
[2023-03-19 05:32:45,067] [INFO] ===== Start GTDB Search =====
[2023-03-19 05:32:45,067] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-a97/markers.fasta)
[2023-03-19 05:32:45,069] [INFO] Task started: Blastn
[2023-03-19 05:32:45,069] [INFO] Running command: blastn -query OceanDNA-a97/markers.fasta -db /var/lib/cwl/stga9574de0-4a7d-4741-9a8a-eefa1f5dcd30/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-a97/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 05:32:45,615] [INFO] Task succeeded: Blastn
[2023-03-19 05:32:45,615] [INFO] Selected 5 target genomes.
[2023-03-19 05:32:45,615] [INFO] Target genome list was writen to OceanDNA-a97/target_genomes_gtdb.txt
[2023-03-19 05:32:45,621] [INFO] Task started: fastANI
[2023-03-19 05:32:45,621] [INFO] Running command: fastANI --query /var/lib/cwl/stg65244fb5-7e01-403e-9620-0c0383e1d720/OceanDNA-a97.fa --refList OceanDNA-a97/target_genomes_gtdb.txt --output OceanDNA-a97/fastani_result_gtdb.tsv --threads 1
[2023-03-19 05:32:46,471] [INFO] Task succeeded: fastANI
[2023-03-19 05:32:46,475] [INFO] Found 5 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-19 05:32:46,475] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_013390375.1	s__Nitrosopelagicus sp000484935	81.3121	170	247	d__Archaea;p__Thermoproteota;c__Nitrososphaeria;o__Nitrososphaerales;f__Nitrosopumilaceae;g__Nitrosopelagicus	95.0	97.75	96.12	0.83	0.80	4	-
GCA_013390765.1	s__Nitrosopelagicus sp013390765	81.2195	109	247	d__Archaea;p__Thermoproteota;c__Nitrososphaeria;o__Nitrososphaerales;f__Nitrosopumilaceae;g__Nitrosopelagicus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013390785.1	s__Nitrosopelagicus sp013390785	81.1881	142	247	d__Archaea;p__Thermoproteota;c__Nitrososphaeria;o__Nitrososphaerales;f__Nitrosopumilaceae;g__Nitrosopelagicus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902606965.1	s__Nitrosopelagicus sp902606965	80.3924	113	247	d__Archaea;p__Thermoproteota;c__Nitrososphaeria;o__Nitrososphaerales;f__Nitrosopumilaceae;g__Nitrosopelagicus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902590655.1	s__Nitrosopelagicus sp902590655	80.0797	127	247	d__Archaea;p__Thermoproteota;c__Nitrososphaeria;o__Nitrososphaerales;f__Nitrosopumilaceae;g__Nitrosopelagicus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-19 05:32:46,475] [INFO] GTDB search result was written to OceanDNA-a97/result_gtdb.tsv
[2023-03-19 05:32:46,476] [INFO] ===== GTDB Search completed =====
[2023-03-19 05:32:46,476] [INFO] DFAST_QC result json was written to OceanDNA-a97/dqc_result.json
[2023-03-19 05:32:46,476] [INFO] DFAST_QC completed!
[2023-03-19 05:32:46,476] [INFO] Total running time: 0h0m26s