[2023-03-18 10:15:15,035] [INFO] DFAST_QC pipeline started.
[2023-03-18 10:15:15,035] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 10:15:15,036] [INFO] DQC Reference Directory: /var/lib/cwl/stg5a68bc7a-028c-403c-a8bd-7c2d7ae5e551/dqc_reference
[2023-03-18 10:15:16,171] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 10:15:16,172] [INFO] Task started: Prodigal
[2023-03-18 10:15:16,172] [INFO] Running command: cat /var/lib/cwl/stg0c48b2a9-d585-4533-8855-e2d7dbeb9afc/OceanDNA-b10540.fa | prodigal -d OceanDNA-b10540/cds.fna -a OceanDNA-b10540/protein.faa -g 11 -q > /dev/null
[2023-03-18 10:15:20,222] [INFO] Task succeeded: Prodigal
[2023-03-18 10:15:20,222] [INFO] Task started: HMMsearch
[2023-03-18 10:15:20,223] [INFO] Running command: hmmsearch --tblout OceanDNA-b10540/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg5a68bc7a-028c-403c-a8bd-7c2d7ae5e551/dqc_reference/reference_markers.hmm OceanDNA-b10540/protein.faa > /dev/null
[2023-03-18 10:15:20,371] [INFO] Task succeeded: HMMsearch
[2023-03-18 10:15:20,371] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg0c48b2a9-d585-4533-8855-e2d7dbeb9afc/OceanDNA-b10540.fa]
[2023-03-18 10:15:20,379] [INFO] Query marker FASTA was written to OceanDNA-b10540/markers.fasta
[2023-03-18 10:15:20,379] [INFO] Task started: Blastn
[2023-03-18 10:15:20,379] [INFO] Running command: blastn -query OceanDNA-b10540/markers.fasta -db /var/lib/cwl/stg5a68bc7a-028c-403c-a8bd-7c2d7ae5e551/dqc_reference/reference_markers.fasta -out OceanDNA-b10540/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 10:15:20,832] [INFO] Task succeeded: Blastn
[2023-03-18 10:15:20,833] [INFO] Selected 18 target genomes.
[2023-03-18 10:15:20,833] [INFO] Target genome list was writen to OceanDNA-b10540/target_genomes.txt
[2023-03-18 10:15:20,906] [INFO] Task started: fastANI
[2023-03-18 10:15:20,906] [INFO] Running command: fastANI --query /var/lib/cwl/stg0c48b2a9-d585-4533-8855-e2d7dbeb9afc/OceanDNA-b10540.fa --refList OceanDNA-b10540/target_genomes.txt --output OceanDNA-b10540/fastani_result.tsv --threads 1
[2023-03-18 10:15:31,161] [INFO] Task succeeded: fastANI
[2023-03-18 10:15:31,161] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg5a68bc7a-028c-403c-a8bd-7c2d7ae5e551/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 10:15:31,162] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg5a68bc7a-028c-403c-a8bd-7c2d7ae5e551/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 10:15:31,162] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 10:15:31,162] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 10:15:31,162] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 10:15:31,162] [INFO] DFAST Taxonomy check result was written to OceanDNA-b10540/tc_result.tsv
[2023-03-18 10:15:31,162] [INFO] ===== Taxonomy check completed =====
[2023-03-18 10:15:31,162] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 10:15:31,162] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg5a68bc7a-028c-403c-a8bd-7c2d7ae5e551/dqc_reference/checkm_data
[2023-03-18 10:15:31,165] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 10:15:31,167] [INFO] Task started: CheckM
[2023-03-18 10:15:31,168] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b10540/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b10540/checkm_input OceanDNA-b10540/checkm_result
[2023-03-18 10:15:47,719] [INFO] Task succeeded: CheckM
[2023-03-18 10:15:47,720] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 27.08%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 10:15:47,721] [INFO] ===== Completeness check finished =====
[2023-03-18 10:15:47,721] [INFO] ===== Start GTDB Search =====
[2023-03-18 10:15:47,722] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b10540/markers.fasta)
[2023-03-18 10:15:47,722] [INFO] Task started: Blastn
[2023-03-18 10:15:47,722] [INFO] Running command: blastn -query OceanDNA-b10540/markers.fasta -db /var/lib/cwl/stg5a68bc7a-028c-403c-a8bd-7c2d7ae5e551/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b10540/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 10:15:48,251] [INFO] Task succeeded: Blastn
[2023-03-18 10:15:48,251] [INFO] Selected 20 target genomes.
[2023-03-18 10:15:48,252] [INFO] Target genome list was writen to OceanDNA-b10540/target_genomes_gtdb.txt
[2023-03-18 10:15:48,270] [INFO] Task started: fastANI
[2023-03-18 10:15:48,270] [INFO] Running command: fastANI --query /var/lib/cwl/stg0c48b2a9-d585-4533-8855-e2d7dbeb9afc/OceanDNA-b10540.fa --refList OceanDNA-b10540/target_genomes_gtdb.txt --output OceanDNA-b10540/fastani_result_gtdb.tsv --threads 1
[2023-03-18 10:15:56,804] [INFO] Task succeeded: fastANI
[2023-03-18 10:15:56,806] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 10:15:56,806] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002698705.1	s__UBA3537 sp002698705	76.8265	55	184	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__UBA3537	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 10:15:56,807] [INFO] GTDB search result was written to OceanDNA-b10540/result_gtdb.tsv
[2023-03-18 10:15:56,808] [INFO] ===== GTDB Search completed =====
[2023-03-18 10:15:56,809] [INFO] DFAST_QC result json was written to OceanDNA-b10540/dqc_result.json
[2023-03-18 10:15:56,809] [INFO] DFAST_QC completed!
[2023-03-18 10:15:56,809] [INFO] Total running time: 0h0m42s