[2023-03-15 05:00:03,355] [INFO] DFAST_QC pipeline started.
[2023-03-15 05:00:03,355] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 05:00:03,356] [INFO] DQC Reference Directory: /var/lib/cwl/stg937e4f18-fec8-47af-81a4-16321eafcf14/dqc_reference
[2023-03-15 05:00:04,450] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 05:00:04,450] [INFO] Task started: Prodigal
[2023-03-15 05:00:04,451] [INFO] Running command: cat /var/lib/cwl/stgef875a46-21e4-417c-bca5-852d214637e9/OceanDNA-b11576.fa | prodigal -d OceanDNA-b11576/cds.fna -a OceanDNA-b11576/protein.faa -g 11 -q > /dev/null
[2023-03-15 05:00:11,682] [INFO] Task succeeded: Prodigal
[2023-03-15 05:00:11,682] [INFO] Task started: HMMsearch
[2023-03-15 05:00:11,682] [INFO] Running command: hmmsearch --tblout OceanDNA-b11576/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg937e4f18-fec8-47af-81a4-16321eafcf14/dqc_reference/reference_markers.hmm OceanDNA-b11576/protein.faa > /dev/null
[2023-03-15 05:00:11,864] [INFO] Task succeeded: HMMsearch
[2023-03-15 05:00:11,864] [INFO] Found 6/6 markers.
[2023-03-15 05:00:11,890] [INFO] Query marker FASTA was written to OceanDNA-b11576/markers.fasta
[2023-03-15 05:00:11,891] [INFO] Task started: Blastn
[2023-03-15 05:00:11,891] [INFO] Running command: blastn -query OceanDNA-b11576/markers.fasta -db /var/lib/cwl/stg937e4f18-fec8-47af-81a4-16321eafcf14/dqc_reference/reference_markers.fasta -out OceanDNA-b11576/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:00:12,415] [INFO] Task succeeded: Blastn
[2023-03-15 05:00:12,421] [INFO] Selected 26 target genomes.
[2023-03-15 05:00:12,422] [INFO] Target genome list was writen to OceanDNA-b11576/target_genomes.txt
[2023-03-15 05:00:12,435] [INFO] Task started: fastANI
[2023-03-15 05:00:12,435] [INFO] Running command: fastANI --query /var/lib/cwl/stgef875a46-21e4-417c-bca5-852d214637e9/OceanDNA-b11576.fa --refList OceanDNA-b11576/target_genomes.txt --output OceanDNA-b11576/fastani_result.tsv --threads 1
[2023-03-15 05:00:26,056] [INFO] Task succeeded: fastANI
[2023-03-15 05:00:26,056] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg937e4f18-fec8-47af-81a4-16321eafcf14/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 05:00:26,056] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg937e4f18-fec8-47af-81a4-16321eafcf14/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 05:00:26,056] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 05:00:26,056] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 05:00:26,056] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 05:00:26,059] [INFO] DFAST Taxonomy check result was written to OceanDNA-b11576/tc_result.tsv
[2023-03-15 05:00:26,061] [INFO] ===== Taxonomy check completed =====
[2023-03-15 05:00:26,062] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 05:00:26,062] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg937e4f18-fec8-47af-81a4-16321eafcf14/dqc_reference/checkm_data
[2023-03-15 05:00:26,064] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 05:00:26,114] [INFO] Task started: CheckM
[2023-03-15 05:00:26,114] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b11576/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b11576/checkm_input OceanDNA-b11576/checkm_result
[2023-03-15 05:00:49,473] [INFO] Task succeeded: CheckM
[2023-03-15 05:00:49,473] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 74.62%
Contamintation: 0.38%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 05:00:49,499] [INFO] ===== Completeness check finished =====
[2023-03-15 05:00:49,499] [INFO] ===== Start GTDB Search =====
[2023-03-15 05:00:49,499] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b11576/markers.fasta)
[2023-03-15 05:00:49,500] [INFO] Task started: Blastn
[2023-03-15 05:00:49,501] [INFO] Running command: blastn -query OceanDNA-b11576/markers.fasta -db /var/lib/cwl/stg937e4f18-fec8-47af-81a4-16321eafcf14/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b11576/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:00:50,244] [INFO] Task succeeded: Blastn
[2023-03-15 05:00:50,252] [INFO] Selected 17 target genomes.
[2023-03-15 05:00:50,252] [INFO] Target genome list was writen to OceanDNA-b11576/target_genomes_gtdb.txt
[2023-03-15 05:00:50,296] [INFO] Task started: fastANI
[2023-03-15 05:00:50,296] [INFO] Running command: fastANI --query /var/lib/cwl/stgef875a46-21e4-417c-bca5-852d214637e9/OceanDNA-b11576.fa --refList OceanDNA-b11576/target_genomes_gtdb.txt --output OceanDNA-b11576/fastani_result_gtdb.tsv --threads 1
[2023-03-15 05:00:56,411] [INFO] Task succeeded: fastANI
[2023-03-15 05:00:56,416] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 05:00:56,417] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002698365.1	s__GCA-002707245 sp002698365	99.726	290	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__GCA-002707245	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002707245.1	s__GCA-002707245 sp002707245	88.1181	266	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__GCA-002707245	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002714185.1	s__SP256 sp002714185	77.5447	143	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__SP256	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002713285.1	s__SP256 sp002713285	77.2414	132	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__SP256	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002701365.1	s__GCA-002701365 sp002701365	77.1049	105	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__GCA-002701365	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002696965.1	s__GCA-2696965 sp002696965	77.0963	84	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__GCA-2696965	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002171975.2	s__GCA-2696965 sp002171975	77.0433	119	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__GCA-2696965	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002722215.1	s__SP256 sp002722215	77.0408	132	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__SP256	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002718895.1	s__GCA-2718895 sp002718895	76.3702	91	364	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__GCA-2718895	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 05:00:56,417] [INFO] GTDB search result was written to OceanDNA-b11576/result_gtdb.tsv
[2023-03-15 05:00:56,417] [INFO] ===== GTDB Search completed =====
[2023-03-15 05:00:56,418] [INFO] DFAST_QC result json was written to OceanDNA-b11576/dqc_result.json
[2023-03-15 05:00:56,418] [INFO] DFAST_QC completed!
[2023-03-15 05:00:56,418] [INFO] Total running time: 0h0m53s