[2023-03-14 13:47:31,790] [INFO] DFAST_QC pipeline started.
[2023-03-14 13:47:31,790] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 13:47:31,790] [INFO] DQC Reference Directory: /var/lib/cwl/stg1b1f4269-5f5c-4942-b9f4-3ce29e0a83c4/dqc_reference
[2023-03-14 13:47:33,025] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 13:47:33,025] [INFO] Task started: Prodigal
[2023-03-14 13:47:33,026] [INFO] Running command: cat /var/lib/cwl/stg4ad52fc9-b873-4348-81fd-50381c0d54e3/OceanDNA-b11605.fa | prodigal -d OceanDNA-b11605/cds.fna -a OceanDNA-b11605/protein.faa -g 11 -q > /dev/null
[2023-03-14 13:47:37,872] [INFO] Task succeeded: Prodigal
[2023-03-14 13:47:37,872] [INFO] Task started: HMMsearch
[2023-03-14 13:47:37,872] [INFO] Running command: hmmsearch --tblout OceanDNA-b11605/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1b1f4269-5f5c-4942-b9f4-3ce29e0a83c4/dqc_reference/reference_markers.hmm OceanDNA-b11605/protein.faa > /dev/null
[2023-03-14 13:47:38,014] [INFO] Task succeeded: HMMsearch
[2023-03-14 13:47:38,014] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg4ad52fc9-b873-4348-81fd-50381c0d54e3/OceanDNA-b11605.fa]
[2023-03-14 13:47:38,022] [INFO] Query marker FASTA was written to OceanDNA-b11605/markers.fasta
[2023-03-14 13:47:38,023] [INFO] Task started: Blastn
[2023-03-14 13:47:38,023] [INFO] Running command: blastn -query OceanDNA-b11605/markers.fasta -db /var/lib/cwl/stg1b1f4269-5f5c-4942-b9f4-3ce29e0a83c4/dqc_reference/reference_markers.fasta -out OceanDNA-b11605/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 13:47:38,507] [INFO] Task succeeded: Blastn
[2023-03-14 13:47:38,508] [INFO] Selected 13 target genomes.
[2023-03-14 13:47:38,508] [INFO] Target genome list was writen to OceanDNA-b11605/target_genomes.txt
[2023-03-14 13:47:38,519] [INFO] Task started: fastANI
[2023-03-14 13:47:38,519] [INFO] Running command: fastANI --query /var/lib/cwl/stg4ad52fc9-b873-4348-81fd-50381c0d54e3/OceanDNA-b11605.fa --refList OceanDNA-b11605/target_genomes.txt --output OceanDNA-b11605/fastani_result.tsv --threads 1
[2023-03-14 13:47:47,074] [INFO] Task succeeded: fastANI
[2023-03-14 13:47:47,075] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1b1f4269-5f5c-4942-b9f4-3ce29e0a83c4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 13:47:47,075] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1b1f4269-5f5c-4942-b9f4-3ce29e0a83c4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 13:47:47,075] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 13:47:47,075] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-14 13:47:47,075] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-14 13:47:47,075] [INFO] DFAST Taxonomy check result was written to OceanDNA-b11605/tc_result.tsv
[2023-03-14 13:47:47,075] [INFO] ===== Taxonomy check completed =====
[2023-03-14 13:47:47,076] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 13:47:47,076] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1b1f4269-5f5c-4942-b9f4-3ce29e0a83c4/dqc_reference/checkm_data
[2023-03-14 13:47:47,086] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 13:47:47,088] [INFO] Task started: CheckM
[2023-03-14 13:47:47,088] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b11605/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b11605/checkm_input OceanDNA-b11605/checkm_result
[2023-03-14 13:48:04,332] [INFO] Task succeeded: CheckM
[2023-03-14 13:48:04,332] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 50.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-14 13:48:04,334] [INFO] ===== Completeness check finished =====
[2023-03-14 13:48:04,334] [INFO] ===== Start GTDB Search =====
[2023-03-14 13:48:04,334] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b11605/markers.fasta)
[2023-03-14 13:48:04,335] [INFO] Task started: Blastn
[2023-03-14 13:48:04,335] [INFO] Running command: blastn -query OceanDNA-b11605/markers.fasta -db /var/lib/cwl/stg1b1f4269-5f5c-4942-b9f4-3ce29e0a83c4/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b11605/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 13:48:05,037] [INFO] Task succeeded: Blastn
[2023-03-14 13:48:05,038] [INFO] Selected 13 target genomes.
[2023-03-14 13:48:05,038] [INFO] Target genome list was writen to OceanDNA-b11605/target_genomes_gtdb.txt
[2023-03-14 13:48:05,048] [INFO] Task started: fastANI
[2023-03-14 13:48:05,048] [INFO] Running command: fastANI --query /var/lib/cwl/stg4ad52fc9-b873-4348-81fd-50381c0d54e3/OceanDNA-b11605.fa --refList OceanDNA-b11605/target_genomes_gtdb.txt --output OceanDNA-b11605/fastani_result_gtdb.tsv --threads 1
[2023-03-14 13:48:08,645] [INFO] Task succeeded: fastANI
[2023-03-14 13:48:08,649] [INFO] Found 3 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-14 13:48:08,649] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002170235.1	s__TMED113 sp002170235	79.1746	109	223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__TMED113	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002715445.1	s__TMED113 sp002715445	79.1088	100	223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__TMED113	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902533035.1	s__GCA-2718035 sp902533035	77.3319	63	223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__TMED113;g__GCA-2718035	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-14 13:48:08,649] [INFO] GTDB search result was written to OceanDNA-b11605/result_gtdb.tsv
[2023-03-14 13:48:08,649] [INFO] ===== GTDB Search completed =====
[2023-03-14 13:48:08,650] [INFO] DFAST_QC result json was written to OceanDNA-b11605/dqc_result.json
[2023-03-14 13:48:08,650] [INFO] DFAST_QC completed!
[2023-03-14 13:48:08,650] [INFO] Total running time: 0h0m37s