[2023-03-19 00:01:03,322] [INFO] DFAST_QC pipeline started.
[2023-03-19 00:01:03,323] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 00:01:03,323] [INFO] DQC Reference Directory: /var/lib/cwl/stg82a2859b-1f72-4bc3-9d4f-af9212849911/dqc_reference
[2023-03-19 00:01:04,563] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 00:01:04,563] [INFO] Task started: Prodigal
[2023-03-19 00:01:04,564] [INFO] Running command: cat /var/lib/cwl/stg7f2b0a6e-c9bf-4615-8055-f2f435ae0db3/OceanDNA-b12047.fa | prodigal -d OceanDNA-b12047/cds.fna -a OceanDNA-b12047/protein.faa -g 11 -q > /dev/null
[2023-03-19 00:01:16,557] [INFO] Task succeeded: Prodigal
[2023-03-19 00:01:16,558] [INFO] Task started: HMMsearch
[2023-03-19 00:01:16,558] [INFO] Running command: hmmsearch --tblout OceanDNA-b12047/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg82a2859b-1f72-4bc3-9d4f-af9212849911/dqc_reference/reference_markers.hmm OceanDNA-b12047/protein.faa > /dev/null
[2023-03-19 00:01:16,769] [INFO] Task succeeded: HMMsearch
[2023-03-19 00:01:16,770] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg7f2b0a6e-c9bf-4615-8055-f2f435ae0db3/OceanDNA-b12047.fa]
[2023-03-19 00:01:16,783] [INFO] Query marker FASTA was written to OceanDNA-b12047/markers.fasta
[2023-03-19 00:01:16,783] [INFO] Task started: Blastn
[2023-03-19 00:01:16,783] [INFO] Running command: blastn -query OceanDNA-b12047/markers.fasta -db /var/lib/cwl/stg82a2859b-1f72-4bc3-9d4f-af9212849911/dqc_reference/reference_markers.fasta -out OceanDNA-b12047/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 00:01:17,313] [INFO] Task succeeded: Blastn
[2023-03-19 00:01:17,314] [INFO] Selected 21 target genomes.
[2023-03-19 00:01:17,314] [INFO] Target genome list was writen to OceanDNA-b12047/target_genomes.txt
[2023-03-19 00:01:17,326] [INFO] Task started: fastANI
[2023-03-19 00:01:17,327] [INFO] Running command: fastANI --query /var/lib/cwl/stg7f2b0a6e-c9bf-4615-8055-f2f435ae0db3/OceanDNA-b12047.fa --refList OceanDNA-b12047/target_genomes.txt --output OceanDNA-b12047/fastani_result.tsv --threads 1
[2023-03-19 00:01:29,790] [INFO] Task succeeded: fastANI
[2023-03-19 00:01:29,791] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg82a2859b-1f72-4bc3-9d4f-af9212849911/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 00:01:29,791] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg82a2859b-1f72-4bc3-9d4f-af9212849911/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 00:01:29,791] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 00:01:29,791] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-19 00:01:29,791] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-19 00:01:29,791] [INFO] DFAST Taxonomy check result was written to OceanDNA-b12047/tc_result.tsv
[2023-03-19 00:01:29,792] [INFO] ===== Taxonomy check completed =====
[2023-03-19 00:01:29,792] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 00:01:29,792] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg82a2859b-1f72-4bc3-9d4f-af9212849911/dqc_reference/checkm_data
[2023-03-19 00:01:29,794] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 00:01:29,798] [INFO] Task started: CheckM
[2023-03-19 00:01:29,798] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b12047/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b12047/checkm_input OceanDNA-b12047/checkm_result
[2023-03-19 00:02:03,803] [INFO] Task succeeded: CheckM
[2023-03-19 00:02:03,804] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 4.17%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-19 00:02:03,806] [INFO] ===== Completeness check finished =====
[2023-03-19 00:02:03,806] [INFO] ===== Start GTDB Search =====
[2023-03-19 00:02:03,806] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b12047/markers.fasta)
[2023-03-19 00:02:03,806] [INFO] Task started: Blastn
[2023-03-19 00:02:03,806] [INFO] Running command: blastn -query OceanDNA-b12047/markers.fasta -db /var/lib/cwl/stg82a2859b-1f72-4bc3-9d4f-af9212849911/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b12047/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 00:02:04,519] [INFO] Task succeeded: Blastn
[2023-03-19 00:02:04,520] [INFO] Selected 19 target genomes.
[2023-03-19 00:02:04,520] [INFO] Target genome list was writen to OceanDNA-b12047/target_genomes_gtdb.txt
[2023-03-19 00:02:04,558] [INFO] Task started: fastANI
[2023-03-19 00:02:04,559] [INFO] Running command: fastANI --query /var/lib/cwl/stg7f2b0a6e-c9bf-4615-8055-f2f435ae0db3/OceanDNA-b12047.fa --refList OceanDNA-b12047/target_genomes_gtdb.txt --output OceanDNA-b12047/fastani_result_gtdb.tsv --threads 1
[2023-03-19 00:02:12,383] [INFO] Task succeeded: fastANI
[2023-03-19 00:02:12,389] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-19 00:02:12,390] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_015660615.1	s__UBA974 sp015660615	95.7514	318	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_018700255.1	s__UBA974 sp018700255	92.7083	348	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002292405.1	s__UBA974 sp002292405	92.008	374	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	99.64	99.64	0.67	0.67	2	-
GCA_002339135.1	s__UBA974 sp002339135	80.1819	365	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905480095.1	s__UBA974 sp905480095	78.6642	105	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002700285.1	s__UBA974 sp002700285	78.13	123	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	99.89	99.89	0.95	0.95	2	-
GCA_015663855.1	s__UBA974 sp012963975	77.935	93	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	99.32	99.24	0.89	0.87	4	-
GCA_002746975.1	s__UBA974 sp002746975	77.2469	117	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002693775.1	s__UBA974 sp002693775	76.6086	61	575	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__UA16;g__UBA974	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-19 00:02:12,390] [INFO] GTDB search result was written to OceanDNA-b12047/result_gtdb.tsv
[2023-03-19 00:02:12,390] [INFO] ===== GTDB Search completed =====
[2023-03-19 00:02:12,391] [INFO] DFAST_QC result json was written to OceanDNA-b12047/dqc_result.json
[2023-03-19 00:02:12,391] [INFO] DFAST_QC completed!
[2023-03-19 00:02:12,391] [INFO] Total running time: 0h1m9s