[2023-03-15 07:29:11,755] [INFO] DFAST_QC pipeline started.
[2023-03-15 07:29:11,756] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 07:29:11,756] [INFO] DQC Reference Directory: /var/lib/cwl/stgbb5f385a-7e70-4c9a-83b5-4ab7ddf04277/dqc_reference
[2023-03-15 07:29:13,590] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 07:29:13,590] [INFO] Task started: Prodigal
[2023-03-15 07:29:13,590] [INFO] Running command: cat /var/lib/cwl/stg7bd1b6bc-2941-4592-bbce-0e5a5e872769/OceanDNA-b13169.fa | prodigal -d OceanDNA-b13169/cds.fna -a OceanDNA-b13169/protein.faa -g 11 -q > /dev/null
[2023-03-15 07:29:21,925] [INFO] Task succeeded: Prodigal
[2023-03-15 07:29:21,925] [INFO] Task started: HMMsearch
[2023-03-15 07:29:21,925] [INFO] Running command: hmmsearch --tblout OceanDNA-b13169/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgbb5f385a-7e70-4c9a-83b5-4ab7ddf04277/dqc_reference/reference_markers.hmm OceanDNA-b13169/protein.faa > /dev/null
[2023-03-15 07:29:22,099] [INFO] Task succeeded: HMMsearch
[2023-03-15 07:29:22,100] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg7bd1b6bc-2941-4592-bbce-0e5a5e872769/OceanDNA-b13169.fa]
[2023-03-15 07:29:22,116] [INFO] Query marker FASTA was written to OceanDNA-b13169/markers.fasta
[2023-03-15 07:29:22,116] [INFO] Task started: Blastn
[2023-03-15 07:29:22,116] [INFO] Running command: blastn -query OceanDNA-b13169/markers.fasta -db /var/lib/cwl/stgbb5f385a-7e70-4c9a-83b5-4ab7ddf04277/dqc_reference/reference_markers.fasta -out OceanDNA-b13169/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 07:29:22,547] [INFO] Task succeeded: Blastn
[2023-03-15 07:29:22,547] [INFO] Selected 15 target genomes.
[2023-03-15 07:29:22,548] [INFO] Target genome list was writen to OceanDNA-b13169/target_genomes.txt
[2023-03-15 07:29:22,557] [INFO] Task started: fastANI
[2023-03-15 07:29:22,557] [INFO] Running command: fastANI --query /var/lib/cwl/stg7bd1b6bc-2941-4592-bbce-0e5a5e872769/OceanDNA-b13169.fa --refList OceanDNA-b13169/target_genomes.txt --output OceanDNA-b13169/fastani_result.tsv --threads 1
[2023-03-15 07:29:29,326] [INFO] Task succeeded: fastANI
[2023-03-15 07:29:29,326] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgbb5f385a-7e70-4c9a-83b5-4ab7ddf04277/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 07:29:29,326] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgbb5f385a-7e70-4c9a-83b5-4ab7ddf04277/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 07:29:29,327] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 07:29:29,327] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 07:29:29,327] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 07:29:29,330] [INFO] DFAST Taxonomy check result was written to OceanDNA-b13169/tc_result.tsv
[2023-03-15 07:29:29,330] [INFO] ===== Taxonomy check completed =====
[2023-03-15 07:29:29,330] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 07:29:29,331] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgbb5f385a-7e70-4c9a-83b5-4ab7ddf04277/dqc_reference/checkm_data
[2023-03-15 07:29:29,334] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 07:29:29,338] [INFO] Task started: CheckM
[2023-03-15 07:29:29,338] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b13169/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b13169/checkm_input OceanDNA-b13169/checkm_result
[2023-03-15 07:29:55,125] [INFO] Task succeeded: CheckM
[2023-03-15 07:29:55,126] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 70.41%
Contamintation: 1.39%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 07:29:55,128] [INFO] ===== Completeness check finished =====
[2023-03-15 07:29:55,128] [INFO] ===== Start GTDB Search =====
[2023-03-15 07:29:55,128] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b13169/markers.fasta)
[2023-03-15 07:29:55,130] [INFO] Task started: Blastn
[2023-03-15 07:29:55,130] [INFO] Running command: blastn -query OceanDNA-b13169/markers.fasta -db /var/lib/cwl/stgbb5f385a-7e70-4c9a-83b5-4ab7ddf04277/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b13169/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 07:29:55,577] [INFO] Task succeeded: Blastn
[2023-03-15 07:29:55,578] [INFO] Selected 15 target genomes.
[2023-03-15 07:29:55,578] [INFO] Target genome list was writen to OceanDNA-b13169/target_genomes_gtdb.txt
[2023-03-15 07:29:55,590] [INFO] Task started: fastANI
[2023-03-15 07:29:55,590] [INFO] Running command: fastANI --query /var/lib/cwl/stg7bd1b6bc-2941-4592-bbce-0e5a5e872769/OceanDNA-b13169.fa --refList OceanDNA-b13169/target_genomes_gtdb.txt --output OceanDNA-b13169/fastani_result_gtdb.tsv --threads 1
[2023-03-15 07:30:01,466] [INFO] Task succeeded: fastANI
[2023-03-15 07:30:01,466] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 07:30:01,466] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-15 07:30:01,466] [INFO] GTDB search result was written to OceanDNA-b13169/result_gtdb.tsv
[2023-03-15 07:30:01,466] [INFO] ===== GTDB Search completed =====
[2023-03-15 07:30:01,467] [INFO] DFAST_QC result json was written to OceanDNA-b13169/dqc_result.json
[2023-03-15 07:30:01,467] [INFO] DFAST_QC completed!
[2023-03-15 07:30:01,467] [INFO] Total running time: 0h0m50s