[2023-03-18 00:05:54,477] [INFO] DFAST_QC pipeline started.
[2023-03-18 00:05:54,477] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 00:05:54,477] [INFO] DQC Reference Directory: /var/lib/cwl/stg360ecbe8-753f-4368-9a4b-0ff179f9f878/dqc_reference
[2023-03-18 00:05:55,626] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 00:05:55,626] [INFO] Task started: Prodigal
[2023-03-18 00:05:55,626] [INFO] Running command: cat /var/lib/cwl/stga8041107-05c6-455a-88a5-ce249612731f/OceanDNA-b13922.fa | prodigal -d OceanDNA-b13922/cds.fna -a OceanDNA-b13922/protein.faa -g 11 -q > /dev/null
[2023-03-18 00:06:16,503] [INFO] Task succeeded: Prodigal
[2023-03-18 00:06:16,503] [INFO] Task started: HMMsearch
[2023-03-18 00:06:16,503] [INFO] Running command: hmmsearch --tblout OceanDNA-b13922/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg360ecbe8-753f-4368-9a4b-0ff179f9f878/dqc_reference/reference_markers.hmm OceanDNA-b13922/protein.faa > /dev/null
[2023-03-18 00:06:16,779] [INFO] Task succeeded: HMMsearch
[2023-03-18 00:06:16,780] [INFO] Found 6/6 markers.
[2023-03-18 00:06:16,801] [INFO] Query marker FASTA was written to OceanDNA-b13922/markers.fasta
[2023-03-18 00:06:16,802] [INFO] Task started: Blastn
[2023-03-18 00:06:16,802] [INFO] Running command: blastn -query OceanDNA-b13922/markers.fasta -db /var/lib/cwl/stg360ecbe8-753f-4368-9a4b-0ff179f9f878/dqc_reference/reference_markers.fasta -out OceanDNA-b13922/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 00:06:17,377] [INFO] Task succeeded: Blastn
[2023-03-18 00:06:17,378] [INFO] Selected 15 target genomes.
[2023-03-18 00:06:17,378] [INFO] Target genome list was writen to OceanDNA-b13922/target_genomes.txt
[2023-03-18 00:06:17,387] [INFO] Task started: fastANI
[2023-03-18 00:06:17,387] [INFO] Running command: fastANI --query /var/lib/cwl/stga8041107-05c6-455a-88a5-ce249612731f/OceanDNA-b13922.fa --refList OceanDNA-b13922/target_genomes.txt --output OceanDNA-b13922/fastani_result.tsv --threads 1
[2023-03-18 00:06:26,583] [INFO] Task succeeded: fastANI
[2023-03-18 00:06:26,584] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg360ecbe8-753f-4368-9a4b-0ff179f9f878/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 00:06:26,584] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg360ecbe8-753f-4368-9a4b-0ff179f9f878/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 00:06:26,584] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 00:06:26,584] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 00:06:26,584] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 00:06:26,584] [INFO] DFAST Taxonomy check result was written to OceanDNA-b13922/tc_result.tsv
[2023-03-18 00:06:26,584] [INFO] ===== Taxonomy check completed =====
[2023-03-18 00:06:26,584] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 00:06:26,585] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg360ecbe8-753f-4368-9a4b-0ff179f9f878/dqc_reference/checkm_data
[2023-03-18 00:06:26,587] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 00:06:26,601] [INFO] Task started: CheckM
[2023-03-18 00:06:26,601] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b13922/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b13922/checkm_input OceanDNA-b13922/checkm_result
[2023-03-18 00:07:18,949] [INFO] Task succeeded: CheckM
[2023-03-18 00:07:18,950] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.46%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-18 00:07:18,978] [INFO] ===== Completeness check finished =====
[2023-03-18 00:07:18,978] [INFO] ===== Start GTDB Search =====
[2023-03-18 00:07:18,978] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b13922/markers.fasta)
[2023-03-18 00:07:18,979] [INFO] Task started: Blastn
[2023-03-18 00:07:18,980] [INFO] Running command: blastn -query OceanDNA-b13922/markers.fasta -db /var/lib/cwl/stg360ecbe8-753f-4368-9a4b-0ff179f9f878/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b13922/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 00:07:19,715] [INFO] Task succeeded: Blastn
[2023-03-18 00:07:19,716] [INFO] Selected 22 target genomes.
[2023-03-18 00:07:19,716] [INFO] Target genome list was writen to OceanDNA-b13922/target_genomes_gtdb.txt
[2023-03-18 00:07:19,727] [INFO] Task started: fastANI
[2023-03-18 00:07:19,727] [INFO] Running command: fastANI --query /var/lib/cwl/stga8041107-05c6-455a-88a5-ce249612731f/OceanDNA-b13922.fa --refList OceanDNA-b13922/target_genomes_gtdb.txt --output OceanDNA-b13922/fastani_result_gtdb.tsv --threads 1
[2023-03-18 00:07:30,834] [INFO] Task succeeded: fastANI
[2023-03-18 00:07:30,837] [INFO] Found 4 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-18 00:07:30,837] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002328865.1	s__UBA2160 sp002328865	99.7962	1027	1117	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__SAR202;f__UBA11138;g__UBA2160	95.0	99.82	99.77	0.91	0.88	7	conclusive
GCA_009838465.1	s__VXRN01 sp009838465	76.0413	54	1117	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__SAR202;f__UBA11138;g__VXRN01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002313895.1	s__UBA1123 sp002313895	75.9985	88	1117	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__SAR202;f__UBA11138;g__UBA1123	95.0	98.51	97.41	0.87	0.77	6	-
GCA_002710885.1	s__UBA1123 sp002710885	75.9364	52	1117	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__SAR202;f__UBA11138;g__UBA1123	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 00:07:30,845] [INFO] GTDB search result was written to OceanDNA-b13922/result_gtdb.tsv
[2023-03-18 00:07:30,848] [INFO] ===== GTDB Search completed =====
[2023-03-18 00:07:30,848] [INFO] DFAST_QC result json was written to OceanDNA-b13922/dqc_result.json
[2023-03-18 00:07:30,849] [INFO] DFAST_QC completed!
[2023-03-18 00:07:30,849] [INFO] Total running time: 0h1m36s