[2023-03-15 05:29:10,779] [INFO] DFAST_QC pipeline started.
[2023-03-15 05:29:10,784] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 05:29:10,784] [INFO] DQC Reference Directory: /var/lib/cwl/stgc5b72df8-e7e4-487e-a704-60e017a55814/dqc_reference
[2023-03-15 05:29:12,382] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 05:29:12,382] [INFO] Task started: Prodigal
[2023-03-15 05:29:12,382] [INFO] Running command: cat /var/lib/cwl/stg55827676-9fad-4be6-b796-20a7b5141ad3/OceanDNA-b14469.fa | prodigal -d OceanDNA-b14469/cds.fna -a OceanDNA-b14469/protein.faa -g 11 -q > /dev/null
[2023-03-15 05:29:22,491] [INFO] Task succeeded: Prodigal
[2023-03-15 05:29:22,491] [INFO] Task started: HMMsearch
[2023-03-15 05:29:22,491] [INFO] Running command: hmmsearch --tblout OceanDNA-b14469/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc5b72df8-e7e4-487e-a704-60e017a55814/dqc_reference/reference_markers.hmm OceanDNA-b14469/protein.faa > /dev/null
[2023-03-15 05:29:22,669] [INFO] Task succeeded: HMMsearch
[2023-03-15 05:29:22,669] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg55827676-9fad-4be6-b796-20a7b5141ad3/OceanDNA-b14469.fa]
[2023-03-15 05:29:22,682] [INFO] Query marker FASTA was written to OceanDNA-b14469/markers.fasta
[2023-03-15 05:29:22,682] [INFO] Task started: Blastn
[2023-03-15 05:29:22,682] [INFO] Running command: blastn -query OceanDNA-b14469/markers.fasta -db /var/lib/cwl/stgc5b72df8-e7e4-487e-a704-60e017a55814/dqc_reference/reference_markers.fasta -out OceanDNA-b14469/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:29:23,183] [INFO] Task succeeded: Blastn
[2023-03-15 05:29:23,184] [INFO] Selected 13 target genomes.
[2023-03-15 05:29:23,184] [INFO] Target genome list was writen to OceanDNA-b14469/target_genomes.txt
[2023-03-15 05:29:23,190] [INFO] Task started: fastANI
[2023-03-15 05:29:23,190] [INFO] Running command: fastANI --query /var/lib/cwl/stg55827676-9fad-4be6-b796-20a7b5141ad3/OceanDNA-b14469.fa --refList OceanDNA-b14469/target_genomes.txt --output OceanDNA-b14469/fastani_result.tsv --threads 1
[2023-03-15 05:29:29,894] [INFO] Task succeeded: fastANI
[2023-03-15 05:29:29,894] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc5b72df8-e7e4-487e-a704-60e017a55814/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 05:29:29,894] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc5b72df8-e7e4-487e-a704-60e017a55814/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 05:29:29,894] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 05:29:29,895] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 05:29:29,895] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 05:29:29,895] [INFO] DFAST Taxonomy check result was written to OceanDNA-b14469/tc_result.tsv
[2023-03-15 05:29:29,895] [INFO] ===== Taxonomy check completed =====
[2023-03-15 05:29:29,895] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 05:29:29,895] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc5b72df8-e7e4-487e-a704-60e017a55814/dqc_reference/checkm_data
[2023-03-15 05:29:29,898] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 05:29:29,902] [INFO] Task started: CheckM
[2023-03-15 05:29:29,902] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b14469/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b14469/checkm_input OceanDNA-b14469/checkm_result
[2023-03-15 05:29:59,890] [INFO] Task succeeded: CheckM
[2023-03-15 05:29:59,890] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 87.50%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 05:29:59,892] [INFO] ===== Completeness check finished =====
[2023-03-15 05:29:59,892] [INFO] ===== Start GTDB Search =====
[2023-03-15 05:29:59,892] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b14469/markers.fasta)
[2023-03-15 05:29:59,893] [INFO] Task started: Blastn
[2023-03-15 05:29:59,893] [INFO] Running command: blastn -query OceanDNA-b14469/markers.fasta -db /var/lib/cwl/stgc5b72df8-e7e4-487e-a704-60e017a55814/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b14469/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:30:00,616] [INFO] Task succeeded: Blastn
[2023-03-15 05:30:00,616] [INFO] Selected 9 target genomes.
[2023-03-15 05:30:00,617] [INFO] Target genome list was writen to OceanDNA-b14469/target_genomes_gtdb.txt
[2023-03-15 05:30:00,641] [INFO] Task started: fastANI
[2023-03-15 05:30:00,641] [INFO] Running command: fastANI --query /var/lib/cwl/stg55827676-9fad-4be6-b796-20a7b5141ad3/OceanDNA-b14469.fa --refList OceanDNA-b14469/target_genomes_gtdb.txt --output OceanDNA-b14469/fastani_result_gtdb.tsv --threads 1
[2023-03-15 05:30:03,930] [INFO] Task succeeded: fastANI
[2023-03-15 05:30:03,934] [INFO] Found 4 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 05:30:03,934] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_009392215.1	s__GCA-002725925 sp009392215	99.6905	470	590	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__UBA1328;g__GCA-002725925	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_008373165.1	s__GCA-002725925 sp009392785	89.9057	458	590	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__UBA1328;g__GCA-002725925	95.0	98.70	97.00	0.83	0.72	6	-
GCA_002687335.1	s__GCA-002725925 sp002687335	87.952	240	590	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__UBA1328;g__GCA-002725925	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002725925.1	s__GCA-002725925 sp002725925	82.1807	335	590	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__UBA1328;g__GCA-002725925	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 05:30:03,934] [INFO] GTDB search result was written to OceanDNA-b14469/result_gtdb.tsv
[2023-03-15 05:30:03,934] [INFO] ===== GTDB Search completed =====
[2023-03-15 05:30:03,935] [INFO] DFAST_QC result json was written to OceanDNA-b14469/dqc_result.json
[2023-03-15 05:30:03,935] [INFO] DFAST_QC completed!
[2023-03-15 05:30:03,935] [INFO] Total running time: 0h0m53s