[2023-03-15 17:24:01,265] [INFO] DFAST_QC pipeline started.
[2023-03-15 17:24:01,265] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 17:24:01,265] [INFO] DQC Reference Directory: /var/lib/cwl/stg62af47e2-7277-440a-a178-5779263ef6c8/dqc_reference
[2023-03-15 17:24:02,458] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 17:24:02,458] [INFO] Task started: Prodigal
[2023-03-15 17:24:02,458] [INFO] Running command: cat /var/lib/cwl/stg4941e462-df90-4045-8a7c-2aaa4c3903c5/OceanDNA-b14697.fa | prodigal -d OceanDNA-b14697/cds.fna -a OceanDNA-b14697/protein.faa -g 11 -q > /dev/null
[2023-03-15 17:24:07,989] [INFO] Task succeeded: Prodigal
[2023-03-15 17:24:07,989] [INFO] Task started: HMMsearch
[2023-03-15 17:24:07,989] [INFO] Running command: hmmsearch --tblout OceanDNA-b14697/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg62af47e2-7277-440a-a178-5779263ef6c8/dqc_reference/reference_markers.hmm OceanDNA-b14697/protein.faa > /dev/null
[2023-03-15 17:24:08,191] [INFO] Task succeeded: HMMsearch
[2023-03-15 17:24:08,191] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg4941e462-df90-4045-8a7c-2aaa4c3903c5/OceanDNA-b14697.fa]
[2023-03-15 17:24:08,202] [INFO] Query marker FASTA was written to OceanDNA-b14697/markers.fasta
[2023-03-15 17:24:08,202] [INFO] Task started: Blastn
[2023-03-15 17:24:08,202] [INFO] Running command: blastn -query OceanDNA-b14697/markers.fasta -db /var/lib/cwl/stg62af47e2-7277-440a-a178-5779263ef6c8/dqc_reference/reference_markers.fasta -out OceanDNA-b14697/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 17:24:08,644] [INFO] Task succeeded: Blastn
[2023-03-15 17:24:08,645] [INFO] Selected 13 target genomes.
[2023-03-15 17:24:08,645] [INFO] Target genome list was writen to OceanDNA-b14697/target_genomes.txt
[2023-03-15 17:24:08,652] [INFO] Task started: fastANI
[2023-03-15 17:24:08,652] [INFO] Running command: fastANI --query /var/lib/cwl/stg4941e462-df90-4045-8a7c-2aaa4c3903c5/OceanDNA-b14697.fa --refList OceanDNA-b14697/target_genomes.txt --output OceanDNA-b14697/fastani_result.tsv --threads 1
[2023-03-15 17:24:15,329] [INFO] Task succeeded: fastANI
[2023-03-15 17:24:15,329] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg62af47e2-7277-440a-a178-5779263ef6c8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 17:24:15,330] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg62af47e2-7277-440a-a178-5779263ef6c8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 17:24:15,330] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 17:24:15,330] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 17:24:15,330] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 17:24:15,330] [INFO] DFAST Taxonomy check result was written to OceanDNA-b14697/tc_result.tsv
[2023-03-15 17:24:15,330] [INFO] ===== Taxonomy check completed =====
[2023-03-15 17:24:15,331] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 17:24:15,331] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg62af47e2-7277-440a-a178-5779263ef6c8/dqc_reference/checkm_data
[2023-03-15 17:24:15,334] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 17:24:15,336] [INFO] Task started: CheckM
[2023-03-15 17:24:15,336] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b14697/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b14697/checkm_input OceanDNA-b14697/checkm_result
[2023-03-15 17:24:35,786] [INFO] Task succeeded: CheckM
[2023-03-15 17:24:35,786] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 17:24:35,787] [INFO] ===== Completeness check finished =====
[2023-03-15 17:24:35,788] [INFO] ===== Start GTDB Search =====
[2023-03-15 17:24:35,788] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b14697/markers.fasta)
[2023-03-15 17:24:35,789] [INFO] Task started: Blastn
[2023-03-15 17:24:35,789] [INFO] Running command: blastn -query OceanDNA-b14697/markers.fasta -db /var/lib/cwl/stg62af47e2-7277-440a-a178-5779263ef6c8/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b14697/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 17:24:36,257] [INFO] Task succeeded: Blastn
[2023-03-15 17:24:36,258] [INFO] Selected 13 target genomes.
[2023-03-15 17:24:36,258] [INFO] Target genome list was writen to OceanDNA-b14697/target_genomes_gtdb.txt
[2023-03-15 17:24:36,274] [INFO] Task started: fastANI
[2023-03-15 17:24:36,274] [INFO] Running command: fastANI --query /var/lib/cwl/stg4941e462-df90-4045-8a7c-2aaa4c3903c5/OceanDNA-b14697.fa --refList OceanDNA-b14697/target_genomes_gtdb.txt --output OceanDNA-b14697/fastani_result_gtdb.tsv --threads 1
[2023-03-15 17:24:39,930] [INFO] Task succeeded: fastANI
[2023-03-15 17:24:39,936] [INFO] Found 8 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 17:24:39,936] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_009937255.1	s__TMED-70 sp009937255	86.0951	323	378	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__TMED-70;g__TMED-70	95.0	96.35	96.35	0.92	0.92	2	-
GCA_002699165.1	s__TMED-70 sp002699165	78.0003	160	378	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__TMED-70;g__TMED-70	95.0	99.00	98.33	0.87	0.75	4	-
GCA_002171675.1	s__TMED-70 sp002171675	77.9673	161	378	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__TMED-70;g__TMED-70	95.0	99.24	99.20	0.89	0.87	3	-
GCA_008373205.1	s__TMED-70 sp008373205	77.8556	169	378	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__TMED-70;g__TMED-70	95.0	99.62	99.62	0.87	0.87	2	-
GCA_002718455.1	s__GCA-2718455 sp002718455	77.7599	97	378	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__TMED-70;g__GCA-2718455	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002721875.1	s__TMED-70 sp002721875	77.5588	134	378	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__TMED-70;g__TMED-70	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002710135.1	s__TMED-70 sp002710135	77.0529	101	378	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__TMED-70;g__TMED-70	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002711965.1	s__TMED-70 sp002711965	77.0007	121	378	d__Bacteria;p__Chloroflexota;c__Dehalococcoidia;o__UBA1151;f__TMED-70;g__TMED-70	95.0	98.97	98.97	0.82	0.82	2	-
--------------------------------------------------------------------------------
[2023-03-15 17:24:39,936] [INFO] GTDB search result was written to OceanDNA-b14697/result_gtdb.tsv
[2023-03-15 17:24:39,936] [INFO] ===== GTDB Search completed =====
[2023-03-15 17:24:39,937] [INFO] DFAST_QC result json was written to OceanDNA-b14697/dqc_result.json
[2023-03-15 17:24:39,937] [INFO] DFAST_QC completed!
[2023-03-15 17:24:39,937] [INFO] Total running time: 0h0m39s