[2023-03-15 21:48:07,254] [INFO] DFAST_QC pipeline started.
[2023-03-15 21:48:07,259] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 21:48:07,259] [INFO] DQC Reference Directory: /var/lib/cwl/stg07c47bf6-edaa-447b-ac0c-7d4673614cc8/dqc_reference
[2023-03-15 21:48:08,903] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 21:48:08,903] [INFO] Task started: Prodigal
[2023-03-15 21:48:08,903] [INFO] Running command: cat /var/lib/cwl/stg004b73a8-da04-495d-9d07-f64725c40ea9/OceanDNA-b15322.fa | prodigal -d OceanDNA-b15322/cds.fna -a OceanDNA-b15322/protein.faa -g 11 -q > /dev/null
[2023-03-15 21:48:12,047] [INFO] Task succeeded: Prodigal
[2023-03-15 21:48:12,047] [INFO] Task started: HMMsearch
[2023-03-15 21:48:12,047] [INFO] Running command: hmmsearch --tblout OceanDNA-b15322/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg07c47bf6-edaa-447b-ac0c-7d4673614cc8/dqc_reference/reference_markers.hmm OceanDNA-b15322/protein.faa > /dev/null
[2023-03-15 21:48:12,222] [INFO] Task succeeded: HMMsearch
[2023-03-15 21:48:12,223] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg004b73a8-da04-495d-9d07-f64725c40ea9/OceanDNA-b15322.fa]
[2023-03-15 21:48:12,230] [INFO] Query marker FASTA was written to OceanDNA-b15322/markers.fasta
[2023-03-15 21:48:12,232] [INFO] Task started: Blastn
[2023-03-15 21:48:12,232] [INFO] Running command: blastn -query OceanDNA-b15322/markers.fasta -db /var/lib/cwl/stg07c47bf6-edaa-447b-ac0c-7d4673614cc8/dqc_reference/reference_markers.fasta -out OceanDNA-b15322/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 21:48:14,491] [INFO] Task succeeded: Blastn
[2023-03-15 21:48:14,491] [INFO] Selected 0 target genomes.
[2023-03-15 21:48:14,491] [INFO] Target genome list was writen to OceanDNA-b15322/target_genomes.txt
[2023-03-15 21:48:14,491] [ERROR] File is empty. [OceanDNA-b15322/target_genomes.txt]
[2023-03-15 21:48:14,492] [ERROR] Task failed. No target genome found.
[2023-03-15 21:48:14,492] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 21:48:14,492] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg07c47bf6-edaa-447b-ac0c-7d4673614cc8/dqc_reference/checkm_data
[2023-03-15 21:48:14,501] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 21:48:14,505] [INFO] Task started: CheckM
[2023-03-15 21:48:14,505] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b15322/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b15322/checkm_input OceanDNA-b15322/checkm_result
[2023-03-15 21:48:31,416] [INFO] Task succeeded: CheckM
[2023-03-15 21:48:31,416] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 41.91%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 21:48:31,418] [INFO] ===== Completeness check finished =====
[2023-03-15 21:48:31,418] [INFO] ===== Start GTDB Search =====
[2023-03-15 21:48:31,418] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b15322/markers.fasta)
[2023-03-15 21:48:31,419] [INFO] Task started: Blastn
[2023-03-15 21:48:31,419] [INFO] Running command: blastn -query OceanDNA-b15322/markers.fasta -db /var/lib/cwl/stg07c47bf6-edaa-447b-ac0c-7d4673614cc8/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b15322/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 21:48:32,303] [INFO] Task succeeded: Blastn
[2023-03-15 21:48:32,304] [INFO] Selected 5 target genomes.
[2023-03-15 21:48:32,304] [INFO] Target genome list was writen to OceanDNA-b15322/target_genomes_gtdb.txt
[2023-03-15 21:48:32,311] [INFO] Task started: fastANI
[2023-03-15 21:48:32,311] [INFO] Running command: fastANI --query /var/lib/cwl/stg004b73a8-da04-495d-9d07-f64725c40ea9/OceanDNA-b15322.fa --refList OceanDNA-b15322/target_genomes_gtdb.txt --output OceanDNA-b15322/fastani_result_gtdb.tsv --threads 1
[2023-03-15 21:48:34,290] [INFO] Task succeeded: fastANI
[2023-03-15 21:48:34,291] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 21:48:34,291] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-15 21:48:34,291] [INFO] GTDB search result was written to OceanDNA-b15322/result_gtdb.tsv
[2023-03-15 21:48:34,291] [INFO] ===== GTDB Search completed =====
[2023-03-15 21:48:34,292] [INFO] DFAST_QC result json was written to OceanDNA-b15322/dqc_result.json
[2023-03-15 21:48:34,292] [INFO] DFAST_QC completed!
[2023-03-15 21:48:34,292] [INFO] Total running time: 0h0m27s