[2023-03-15 09:48:16,399] [INFO] DFAST_QC pipeline started.
[2023-03-15 09:48:16,399] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 09:48:16,399] [INFO] DQC Reference Directory: /var/lib/cwl/stg1a27d2e4-6fb8-4858-b2d1-4434b5db2fe5/dqc_reference
[2023-03-15 09:48:18,053] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 09:48:18,054] [INFO] Task started: Prodigal
[2023-03-15 09:48:18,054] [INFO] Running command: cat /var/lib/cwl/stgcb01016c-9823-4090-ab0f-53d73feea371/OceanDNA-b16018.fa | prodigal -d OceanDNA-b16018/cds.fna -a OceanDNA-b16018/protein.faa -g 11 -q > /dev/null
[2023-03-15 09:48:27,587] [INFO] Task succeeded: Prodigal
[2023-03-15 09:48:27,588] [INFO] Task started: HMMsearch
[2023-03-15 09:48:27,588] [INFO] Running command: hmmsearch --tblout OceanDNA-b16018/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1a27d2e4-6fb8-4858-b2d1-4434b5db2fe5/dqc_reference/reference_markers.hmm OceanDNA-b16018/protein.faa > /dev/null
[2023-03-15 09:48:27,817] [INFO] Task succeeded: HMMsearch
[2023-03-15 09:48:27,818] [INFO] Found 6/6 markers.
[2023-03-15 09:48:27,832] [INFO] Query marker FASTA was written to OceanDNA-b16018/markers.fasta
[2023-03-15 09:48:27,833] [INFO] Task started: Blastn
[2023-03-15 09:48:27,833] [INFO] Running command: blastn -query OceanDNA-b16018/markers.fasta -db /var/lib/cwl/stg1a27d2e4-6fb8-4858-b2d1-4434b5db2fe5/dqc_reference/reference_markers.fasta -out OceanDNA-b16018/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 09:48:28,383] [INFO] Task succeeded: Blastn
[2023-03-15 09:48:28,385] [INFO] Selected 28 target genomes.
[2023-03-15 09:48:28,385] [INFO] Target genome list was writen to OceanDNA-b16018/target_genomes.txt
[2023-03-15 09:48:28,402] [INFO] Task started: fastANI
[2023-03-15 09:48:28,402] [INFO] Running command: fastANI --query /var/lib/cwl/stgcb01016c-9823-4090-ab0f-53d73feea371/OceanDNA-b16018.fa --refList OceanDNA-b16018/target_genomes.txt --output OceanDNA-b16018/fastani_result.tsv --threads 1
[2023-03-15 09:48:47,127] [INFO] Task succeeded: fastANI
[2023-03-15 09:48:47,127] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1a27d2e4-6fb8-4858-b2d1-4434b5db2fe5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 09:48:47,128] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1a27d2e4-6fb8-4858-b2d1-4434b5db2fe5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 09:48:47,128] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 09:48:47,128] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 09:48:47,128] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 09:48:47,128] [INFO] DFAST Taxonomy check result was written to OceanDNA-b16018/tc_result.tsv
[2023-03-15 09:48:47,128] [INFO] ===== Taxonomy check completed =====
[2023-03-15 09:48:47,128] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 09:48:47,128] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1a27d2e4-6fb8-4858-b2d1-4434b5db2fe5/dqc_reference/checkm_data
[2023-03-15 09:48:47,131] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 09:48:47,137] [INFO] Task started: CheckM
[2023-03-15 09:48:47,137] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b16018/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b16018/checkm_input OceanDNA-b16018/checkm_result
[2023-03-15 09:49:15,740] [INFO] Task succeeded: CheckM
[2023-03-15 09:49:15,740] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.46%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 09:49:15,742] [INFO] ===== Completeness check finished =====
[2023-03-15 09:49:15,742] [INFO] ===== Start GTDB Search =====
[2023-03-15 09:49:15,742] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b16018/markers.fasta)
[2023-03-15 09:49:15,742] [INFO] Task started: Blastn
[2023-03-15 09:49:15,743] [INFO] Running command: blastn -query OceanDNA-b16018/markers.fasta -db /var/lib/cwl/stg1a27d2e4-6fb8-4858-b2d1-4434b5db2fe5/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b16018/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 09:49:16,447] [INFO] Task succeeded: Blastn
[2023-03-15 09:49:16,448] [INFO] Selected 29 target genomes.
[2023-03-15 09:49:16,448] [INFO] Target genome list was writen to OceanDNA-b16018/target_genomes_gtdb.txt
[2023-03-15 09:49:16,830] [INFO] Task started: fastANI
[2023-03-15 09:49:16,830] [INFO] Running command: fastANI --query /var/lib/cwl/stgcb01016c-9823-4090-ab0f-53d73feea371/OceanDNA-b16018.fa --refList OceanDNA-b16018/target_genomes_gtdb.txt --output OceanDNA-b16018/fastani_result_gtdb.tsv --threads 1
[2023-03-15 09:49:31,047] [INFO] Task succeeded: fastANI
[2023-03-15 09:49:31,049] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 09:49:31,049] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018654125.1	s__JABIME01 sp018654125	98.5984	455	592	d__Bacteria;p__Cloacimonadota;c__Cloacimonadia;o__Cloacimonadales;f__TCS61;g__JABIME01	95.0	98.84	98.78	0.81	0.79	3	conclusive
--------------------------------------------------------------------------------
[2023-03-15 09:49:31,049] [INFO] GTDB search result was written to OceanDNA-b16018/result_gtdb.tsv
[2023-03-15 09:49:31,049] [INFO] ===== GTDB Search completed =====
[2023-03-15 09:49:31,050] [INFO] DFAST_QC result json was written to OceanDNA-b16018/dqc_result.json
[2023-03-15 09:49:31,050] [INFO] DFAST_QC completed!
[2023-03-15 09:49:31,050] [INFO] Total running time: 0h1m15s