[2023-03-18 05:05:06,087] [INFO] DFAST_QC pipeline started.
[2023-03-18 05:05:06,087] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 05:05:06,088] [INFO] DQC Reference Directory: /var/lib/cwl/stg3bde9258-1a41-4d8a-9dfb-62eade0ad62e/dqc_reference
[2023-03-18 05:05:07,204] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 05:05:07,204] [INFO] Task started: Prodigal
[2023-03-18 05:05:07,205] [INFO] Running command: cat /var/lib/cwl/stg55d693c3-0ae6-47b9-a3e9-483e85048116/OceanDNA-b16135.fa | prodigal -d OceanDNA-b16135/cds.fna -a OceanDNA-b16135/protein.faa -g 11 -q > /dev/null
[2023-03-18 05:05:29,117] [INFO] Task succeeded: Prodigal
[2023-03-18 05:05:29,118] [INFO] Task started: HMMsearch
[2023-03-18 05:05:29,118] [INFO] Running command: hmmsearch --tblout OceanDNA-b16135/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3bde9258-1a41-4d8a-9dfb-62eade0ad62e/dqc_reference/reference_markers.hmm OceanDNA-b16135/protein.faa > /dev/null
[2023-03-18 05:05:29,395] [INFO] Task succeeded: HMMsearch
[2023-03-18 05:05:29,396] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg55d693c3-0ae6-47b9-a3e9-483e85048116/OceanDNA-b16135.fa]
[2023-03-18 05:05:29,433] [INFO] Query marker FASTA was written to OceanDNA-b16135/markers.fasta
[2023-03-18 05:05:29,434] [INFO] Task started: Blastn
[2023-03-18 05:05:29,435] [INFO] Running command: blastn -query OceanDNA-b16135/markers.fasta -db /var/lib/cwl/stg3bde9258-1a41-4d8a-9dfb-62eade0ad62e/dqc_reference/reference_markers.fasta -out OceanDNA-b16135/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 05:05:29,975] [INFO] Task succeeded: Blastn
[2023-03-18 05:05:29,980] [INFO] Selected 25 target genomes.
[2023-03-18 05:05:29,980] [INFO] Target genome list was writen to OceanDNA-b16135/target_genomes.txt
[2023-03-18 05:05:29,998] [INFO] Task started: fastANI
[2023-03-18 05:05:29,998] [INFO] Running command: fastANI --query /var/lib/cwl/stg55d693c3-0ae6-47b9-a3e9-483e85048116/OceanDNA-b16135.fa --refList OceanDNA-b16135/target_genomes.txt --output OceanDNA-b16135/fastani_result.tsv --threads 1
[2023-03-18 05:05:48,909] [INFO] Task succeeded: fastANI
[2023-03-18 05:05:48,910] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3bde9258-1a41-4d8a-9dfb-62eade0ad62e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 05:05:48,910] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3bde9258-1a41-4d8a-9dfb-62eade0ad62e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 05:05:48,914] [INFO] Found 2 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 05:05:48,914] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-18 05:05:48,914] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Waterburya agarophytonicola	strain=KI4	GCA_020640915.1	2886916	2886916	type	True	77.1475	363	1443	95	below_threshold
Stanieria cyanosphaera	strain=PCC 7437	GCA_000317575.1	102116	102116	type	True	75.6941	91	1443	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-18 05:05:48,915] [INFO] DFAST Taxonomy check result was written to OceanDNA-b16135/tc_result.tsv
[2023-03-18 05:05:48,918] [INFO] ===== Taxonomy check completed =====
[2023-03-18 05:05:48,918] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 05:05:48,918] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3bde9258-1a41-4d8a-9dfb-62eade0ad62e/dqc_reference/checkm_data
[2023-03-18 05:05:48,919] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 05:05:48,994] [INFO] Task started: CheckM
[2023-03-18 05:05:48,994] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b16135/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b16135/checkm_input OceanDNA-b16135/checkm_result
[2023-03-18 05:06:43,450] [INFO] Task succeeded: CheckM
[2023-03-18 05:06:43,450] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 70.83%
Contamintation: 5.21%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-03-18 05:06:43,491] [INFO] ===== Completeness check finished =====
[2023-03-18 05:06:43,491] [INFO] ===== Start GTDB Search =====
[2023-03-18 05:06:43,491] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b16135/markers.fasta)
[2023-03-18 05:06:43,492] [INFO] Task started: Blastn
[2023-03-18 05:06:43,492] [INFO] Running command: blastn -query OceanDNA-b16135/markers.fasta -db /var/lib/cwl/stg3bde9258-1a41-4d8a-9dfb-62eade0ad62e/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b16135/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 05:06:44,335] [INFO] Task succeeded: Blastn
[2023-03-18 05:06:44,341] [INFO] Selected 25 target genomes.
[2023-03-18 05:06:44,341] [INFO] Target genome list was writen to OceanDNA-b16135/target_genomes_gtdb.txt
[2023-03-18 05:06:44,542] [INFO] Task started: fastANI
[2023-03-18 05:06:44,543] [INFO] Running command: fastANI --query /var/lib/cwl/stg55d693c3-0ae6-47b9-a3e9-483e85048116/OceanDNA-b16135.fa --refList OceanDNA-b16135/target_genomes_gtdb.txt --output OceanDNA-b16135/fastani_result_gtdb.tsv --threads 1
[2023-03-18 05:07:07,886] [INFO] Task succeeded: fastANI
[2023-03-18 05:07:07,891] [INFO] Found 8 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 05:07:07,892] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_012034765.1	s__Pleurocapsa sp012034765	78.0765	468	1443	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	99.36	99.13	0.90	0.87	3	-
GCF_003003995.1	s__Pleurocapsa sp003003995	78.025	467	1443	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015207195.1	s__Pleurocapsa sp015207195	77.3255	429	1443	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000332195.1	s__Pleurocapsa sp000332195	76.7281	277	1443	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002368355.1	s__Pleurocapsa sp002368355	76.4182	205	1443	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000756305.1	s__Myxosarcina sp000756305	76.2353	191	1443	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Myxosarcina	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000317575.1	s__Stanieria cyanosphaera	75.7346	88	1443	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Stanieria	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000332055.1	s__Xenococcus sp000332055	75.4215	72	1443	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Xenococcus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 05:07:07,895] [INFO] GTDB search result was written to OceanDNA-b16135/result_gtdb.tsv
[2023-03-18 05:07:07,896] [INFO] ===== GTDB Search completed =====
[2023-03-18 05:07:07,898] [INFO] DFAST_QC result json was written to OceanDNA-b16135/dqc_result.json
[2023-03-18 05:07:07,898] [INFO] DFAST_QC completed!
[2023-03-18 05:07:07,899] [INFO] Total running time: 0h2m2s