[2023-03-14 13:09:10,231] [INFO] DFAST_QC pipeline started.
[2023-03-14 13:09:10,231] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 13:09:10,232] [INFO] DQC Reference Directory: /var/lib/cwl/stg9bc8cf32-2536-425b-a8d8-98fd6ae8e3cc/dqc_reference
[2023-03-14 13:09:11,512] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 13:09:11,512] [INFO] Task started: Prodigal
[2023-03-14 13:09:11,512] [INFO] Running command: cat /var/lib/cwl/stg764d7690-cd3f-4160-a6bd-d51e97793095/OceanDNA-b16146.fa | prodigal -d OceanDNA-b16146/cds.fna -a OceanDNA-b16146/protein.faa -g 11 -q > /dev/null
[2023-03-14 13:09:38,772] [INFO] Task succeeded: Prodigal
[2023-03-14 13:09:38,773] [INFO] Task started: HMMsearch
[2023-03-14 13:09:38,773] [INFO] Running command: hmmsearch --tblout OceanDNA-b16146/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9bc8cf32-2536-425b-a8d8-98fd6ae8e3cc/dqc_reference/reference_markers.hmm OceanDNA-b16146/protein.faa > /dev/null
[2023-03-14 13:09:39,184] [INFO] Task succeeded: HMMsearch
[2023-03-14 13:09:39,185] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg764d7690-cd3f-4160-a6bd-d51e97793095/OceanDNA-b16146.fa]
[2023-03-14 13:09:39,210] [INFO] Query marker FASTA was written to OceanDNA-b16146/markers.fasta
[2023-03-14 13:09:39,211] [INFO] Task started: Blastn
[2023-03-14 13:09:39,211] [INFO] Running command: blastn -query OceanDNA-b16146/markers.fasta -db /var/lib/cwl/stg9bc8cf32-2536-425b-a8d8-98fd6ae8e3cc/dqc_reference/reference_markers.fasta -out OceanDNA-b16146/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 13:09:39,743] [INFO] Task succeeded: Blastn
[2023-03-14 13:09:39,744] [INFO] Selected 19 target genomes.
[2023-03-14 13:09:39,745] [INFO] Target genome list was writen to OceanDNA-b16146/target_genomes.txt
[2023-03-14 13:09:39,763] [INFO] Task started: fastANI
[2023-03-14 13:09:39,763] [INFO] Running command: fastANI --query /var/lib/cwl/stg764d7690-cd3f-4160-a6bd-d51e97793095/OceanDNA-b16146.fa --refList OceanDNA-b16146/target_genomes.txt --output OceanDNA-b16146/fastani_result.tsv --threads 1
[2023-03-14 13:09:52,969] [INFO] Task succeeded: fastANI
[2023-03-14 13:09:52,969] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9bc8cf32-2536-425b-a8d8-98fd6ae8e3cc/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 13:09:52,970] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9bc8cf32-2536-425b-a8d8-98fd6ae8e3cc/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 13:09:52,973] [INFO] Found 1 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 13:09:52,973] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-14 13:09:52,973] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Waterburya agarophytonicola	strain=KI4	GCA_020640915.1	2886916	2886916	type	True	76.5872	214	1167	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-14 13:09:52,974] [INFO] DFAST Taxonomy check result was written to OceanDNA-b16146/tc_result.tsv
[2023-03-14 13:09:52,974] [INFO] ===== Taxonomy check completed =====
[2023-03-14 13:09:52,974] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 13:09:52,974] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9bc8cf32-2536-425b-a8d8-98fd6ae8e3cc/dqc_reference/checkm_data
[2023-03-14 13:09:52,975] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 13:09:52,982] [INFO] Task started: CheckM
[2023-03-14 13:09:52,982] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b16146/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b16146/checkm_input OceanDNA-b16146/checkm_result
[2023-03-14 13:10:47,443] [INFO] Task succeeded: CheckM
[2023-03-14 13:10:47,443] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 55.38%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-14 13:10:47,446] [INFO] ===== Completeness check finished =====
[2023-03-14 13:10:47,446] [INFO] ===== Start GTDB Search =====
[2023-03-14 13:10:47,446] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b16146/markers.fasta)
[2023-03-14 13:10:47,447] [INFO] Task started: Blastn
[2023-03-14 13:10:47,447] [INFO] Running command: blastn -query OceanDNA-b16146/markers.fasta -db /var/lib/cwl/stg9bc8cf32-2536-425b-a8d8-98fd6ae8e3cc/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b16146/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 13:10:48,170] [INFO] Task succeeded: Blastn
[2023-03-14 13:10:48,171] [INFO] Selected 21 target genomes.
[2023-03-14 13:10:48,171] [INFO] Target genome list was writen to OceanDNA-b16146/target_genomes_gtdb.txt
[2023-03-14 13:10:48,204] [INFO] Task started: fastANI
[2023-03-14 13:10:48,204] [INFO] Running command: fastANI --query /var/lib/cwl/stg764d7690-cd3f-4160-a6bd-d51e97793095/OceanDNA-b16146.fa --refList OceanDNA-b16146/target_genomes_gtdb.txt --output OceanDNA-b16146/fastani_result_gtdb.tsv --threads 1
[2023-03-14 13:11:05,404] [INFO] Task succeeded: fastANI
[2023-03-14 13:11:05,409] [INFO] Found 7 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-14 13:11:05,410] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003003995.1	s__Pleurocapsa sp003003995	77.7166	368	1167	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	N/A	N/A	N/A	N/A	1	-
GCA_012034765.1	s__Pleurocapsa sp012034765	77.3993	385	1167	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	99.36	99.13	0.90	0.87	3	-
GCA_015207195.1	s__Pleurocapsa sp015207195	77.0397	269	1167	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000332195.1	s__Pleurocapsa sp000332195	76.317	197	1167	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002368355.1	s__Pleurocapsa sp002368355	76.0245	125	1167	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Pleurocapsa	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000756305.1	s__Myxosarcina sp000756305	75.7687	108	1167	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Myxosarcina	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900659865.1	s__Hyella patelloides	75.7026	106	1167	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Xenococcaceae;g__Hyella	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-14 13:11:05,410] [INFO] GTDB search result was written to OceanDNA-b16146/result_gtdb.tsv
[2023-03-14 13:11:05,410] [INFO] ===== GTDB Search completed =====
[2023-03-14 13:11:05,411] [INFO] DFAST_QC result json was written to OceanDNA-b16146/dqc_result.json
[2023-03-14 13:11:05,411] [INFO] DFAST_QC completed!
[2023-03-14 13:11:05,411] [INFO] Total running time: 0h1m55s