[2023-03-16 09:13:21,780] [INFO] DFAST_QC pipeline started.
[2023-03-16 09:13:21,781] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 09:13:21,781] [INFO] DQC Reference Directory: /var/lib/cwl/stgb4920400-29ef-4693-90cb-8a3eef3780af/dqc_reference
[2023-03-16 09:13:22,883] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 09:13:22,884] [INFO] Task started: Prodigal
[2023-03-16 09:13:22,884] [INFO] Running command: cat /var/lib/cwl/stgcc19d1e6-785b-40b3-958b-6280ac29be00/OceanDNA-b16624.fa | prodigal -d OceanDNA-b16624/cds.fna -a OceanDNA-b16624/protein.faa -g 11 -q > /dev/null
[2023-03-16 09:13:29,670] [INFO] Task succeeded: Prodigal
[2023-03-16 09:13:29,670] [INFO] Task started: HMMsearch
[2023-03-16 09:13:29,671] [INFO] Running command: hmmsearch --tblout OceanDNA-b16624/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb4920400-29ef-4693-90cb-8a3eef3780af/dqc_reference/reference_markers.hmm OceanDNA-b16624/protein.faa > /dev/null
[2023-03-16 09:13:29,797] [INFO] Task succeeded: HMMsearch
[2023-03-16 09:13:29,797] [WARNING] Found 2/6 markers. [/var/lib/cwl/stgcc19d1e6-785b-40b3-958b-6280ac29be00/OceanDNA-b16624.fa]
[2023-03-16 09:13:29,814] [INFO] Query marker FASTA was written to OceanDNA-b16624/markers.fasta
[2023-03-16 09:13:29,814] [INFO] Task started: Blastn
[2023-03-16 09:13:29,814] [INFO] Running command: blastn -query OceanDNA-b16624/markers.fasta -db /var/lib/cwl/stgb4920400-29ef-4693-90cb-8a3eef3780af/dqc_reference/reference_markers.fasta -out OceanDNA-b16624/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 09:13:30,261] [INFO] Task succeeded: Blastn
[2023-03-16 09:13:30,267] [INFO] Selected 10 target genomes.
[2023-03-16 09:13:30,267] [INFO] Target genome list was writen to OceanDNA-b16624/target_genomes.txt
[2023-03-16 09:13:30,272] [INFO] Task started: fastANI
[2023-03-16 09:13:30,272] [INFO] Running command: fastANI --query /var/lib/cwl/stgcc19d1e6-785b-40b3-958b-6280ac29be00/OceanDNA-b16624.fa --refList OceanDNA-b16624/target_genomes.txt --output OceanDNA-b16624/fastani_result.tsv --threads 1
[2023-03-16 09:13:36,169] [INFO] Task succeeded: fastANI
[2023-03-16 09:13:36,169] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb4920400-29ef-4693-90cb-8a3eef3780af/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 09:13:36,169] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb4920400-29ef-4693-90cb-8a3eef3780af/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 09:13:36,169] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 09:13:36,169] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 09:13:36,169] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 09:13:36,171] [INFO] DFAST Taxonomy check result was written to OceanDNA-b16624/tc_result.tsv
[2023-03-16 09:13:36,173] [INFO] ===== Taxonomy check completed =====
[2023-03-16 09:13:36,173] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 09:13:36,173] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb4920400-29ef-4693-90cb-8a3eef3780af/dqc_reference/checkm_data
[2023-03-16 09:13:36,176] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 09:13:36,206] [INFO] Task started: CheckM
[2023-03-16 09:13:36,206] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b16624/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b16624/checkm_input OceanDNA-b16624/checkm_result
[2023-03-16 09:14:00,383] [INFO] Task succeeded: CheckM
[2023-03-16 09:14:00,384] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 40.51%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 09:14:00,437] [INFO] ===== Completeness check finished =====
[2023-03-16 09:14:00,437] [INFO] ===== Start GTDB Search =====
[2023-03-16 09:14:00,437] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b16624/markers.fasta)
[2023-03-16 09:14:00,438] [INFO] Task started: Blastn
[2023-03-16 09:14:00,438] [INFO] Running command: blastn -query OceanDNA-b16624/markers.fasta -db /var/lib/cwl/stgb4920400-29ef-4693-90cb-8a3eef3780af/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b16624/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 09:14:00,948] [INFO] Task succeeded: Blastn
[2023-03-16 09:14:00,950] [INFO] Selected 7 target genomes.
[2023-03-16 09:14:00,950] [INFO] Target genome list was writen to OceanDNA-b16624/target_genomes_gtdb.txt
[2023-03-16 09:14:00,957] [INFO] Task started: fastANI
[2023-03-16 09:14:00,957] [INFO] Running command: fastANI --query /var/lib/cwl/stgcc19d1e6-785b-40b3-958b-6280ac29be00/OceanDNA-b16624.fa --refList OceanDNA-b16624/target_genomes_gtdb.txt --output OceanDNA-b16624/fastani_result_gtdb.tsv --threads 1
[2023-03-16 09:14:03,813] [INFO] Task succeeded: fastANI
[2023-03-16 09:14:03,817] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 09:14:03,818] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001040845.1	s__Synechococcus_C sp001040845	99.091	78	78	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__PCC-6307;f__Cyanobiaceae;g__Synechococcus_C	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_000014585.1	s__Synechococcus_C sp000014585	91.5617	78	78	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__PCC-6307;f__Cyanobiaceae;g__Synechococcus_C	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003210315.1	s__Synechococcus_C sp003210315	87.9958	53	78	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__PCC-6307;f__Cyanobiaceae;g__Synechococcus_C	95.0	96.13	96.13	0.52	0.52	2	-
GCF_014279775.1	s__Synechococcus_C sp002500205	87.7452	76	78	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__PCC-6307;f__Cyanobiaceae;g__Synechococcus_C	95.0	99.03	97.76	0.95	0.86	9	-
GCF_014279835.1	s__Synechococcus_C sp014279835	87.1922	77	78	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__PCC-6307;f__Cyanobiaceae;g__Synechococcus_C	95.0	96.93	96.36	0.92	0.87	4	-
GCF_000230675.1	s__Synechococcus_C sp000230675	86.3076	78	78	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__PCC-6307;f__Cyanobiaceae;g__Synechococcus_C	95.0	96.29	95.09	0.90	0.84	8	-
--------------------------------------------------------------------------------
[2023-03-16 09:14:03,821] [INFO] GTDB search result was written to OceanDNA-b16624/result_gtdb.tsv
[2023-03-16 09:14:03,824] [INFO] ===== GTDB Search completed =====
[2023-03-16 09:14:03,828] [INFO] DFAST_QC result json was written to OceanDNA-b16624/dqc_result.json
[2023-03-16 09:14:03,828] [INFO] DFAST_QC completed!
[2023-03-16 09:14:03,828] [INFO] Total running time: 0h0m42s