[2023-03-18 22:17:12,621] [INFO] DFAST_QC pipeline started.
[2023-03-18 22:17:12,622] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 22:17:12,622] [INFO] DQC Reference Directory: /var/lib/cwl/stg2596b3fd-2448-4d20-a7fa-62d375b3f745/dqc_reference
[2023-03-18 22:17:13,705] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 22:17:13,705] [INFO] Task started: Prodigal
[2023-03-18 22:17:13,705] [INFO] Running command: cat /var/lib/cwl/stgbf7c535e-60a1-45c8-8305-df53feb1c27a/OceanDNA-b17149.fa | prodigal -d OceanDNA-b17149/cds.fna -a OceanDNA-b17149/protein.faa -g 11 -q > /dev/null
[2023-03-18 22:17:19,864] [INFO] Task succeeded: Prodigal
[2023-03-18 22:17:19,864] [INFO] Task started: HMMsearch
[2023-03-18 22:17:19,865] [INFO] Running command: hmmsearch --tblout OceanDNA-b17149/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2596b3fd-2448-4d20-a7fa-62d375b3f745/dqc_reference/reference_markers.hmm OceanDNA-b17149/protein.faa > /dev/null
[2023-03-18 22:17:20,024] [INFO] Task succeeded: HMMsearch
[2023-03-18 22:17:20,025] [INFO] Found 6/6 markers.
[2023-03-18 22:17:20,062] [INFO] Query marker FASTA was written to OceanDNA-b17149/markers.fasta
[2023-03-18 22:17:20,063] [INFO] Task started: Blastn
[2023-03-18 22:17:20,063] [INFO] Running command: blastn -query OceanDNA-b17149/markers.fasta -db /var/lib/cwl/stg2596b3fd-2448-4d20-a7fa-62d375b3f745/dqc_reference/reference_markers.fasta -out OceanDNA-b17149/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 22:17:20,570] [INFO] Task succeeded: Blastn
[2023-03-18 22:17:20,572] [INFO] Selected 11 target genomes.
[2023-03-18 22:17:20,573] [INFO] Target genome list was writen to OceanDNA-b17149/target_genomes.txt
[2023-03-18 22:17:20,578] [INFO] Task started: fastANI
[2023-03-18 22:17:20,579] [INFO] Running command: fastANI --query /var/lib/cwl/stgbf7c535e-60a1-45c8-8305-df53feb1c27a/OceanDNA-b17149.fa --refList OceanDNA-b17149/target_genomes.txt --output OceanDNA-b17149/fastani_result.tsv --threads 1
[2023-03-18 22:17:25,899] [INFO] Task succeeded: fastANI
[2023-03-18 22:17:25,900] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2596b3fd-2448-4d20-a7fa-62d375b3f745/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 22:17:25,900] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2596b3fd-2448-4d20-a7fa-62d375b3f745/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 22:17:25,900] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 22:17:25,900] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 22:17:25,900] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 22:17:25,900] [INFO] DFAST Taxonomy check result was written to OceanDNA-b17149/tc_result.tsv
[2023-03-18 22:17:25,901] [INFO] ===== Taxonomy check completed =====
[2023-03-18 22:17:25,901] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 22:17:25,901] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2596b3fd-2448-4d20-a7fa-62d375b3f745/dqc_reference/checkm_data
[2023-03-18 22:17:25,904] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 22:17:25,910] [INFO] Task started: CheckM
[2023-03-18 22:17:25,910] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b17149/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b17149/checkm_input OceanDNA-b17149/checkm_result
[2023-03-18 22:17:46,711] [INFO] Task succeeded: CheckM
[2023-03-18 22:17:46,712] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 79.17%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 22:17:46,714] [INFO] ===== Completeness check finished =====
[2023-03-18 22:17:46,714] [INFO] ===== Start GTDB Search =====
[2023-03-18 22:17:46,715] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b17149/markers.fasta)
[2023-03-18 22:17:46,715] [INFO] Task started: Blastn
[2023-03-18 22:17:46,715] [INFO] Running command: blastn -query OceanDNA-b17149/markers.fasta -db /var/lib/cwl/stg2596b3fd-2448-4d20-a7fa-62d375b3f745/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b17149/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 22:17:47,448] [INFO] Task succeeded: Blastn
[2023-03-18 22:17:47,449] [INFO] Selected 17 target genomes.
[2023-03-18 22:17:47,449] [INFO] Target genome list was writen to OceanDNA-b17149/target_genomes_gtdb.txt
[2023-03-18 22:17:47,466] [INFO] Task started: fastANI
[2023-03-18 22:17:47,466] [INFO] Running command: fastANI --query /var/lib/cwl/stgbf7c535e-60a1-45c8-8305-df53feb1c27a/OceanDNA-b17149.fa --refList OceanDNA-b17149/target_genomes_gtdb.txt --output OceanDNA-b17149/fastani_result_gtdb.tsv --threads 1
[2023-03-18 22:17:52,747] [INFO] Task succeeded: fastANI
[2023-03-18 22:17:52,749] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-18 22:17:52,750] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018656005.1	s__JABHZW01 sp018656005	99.8442	355	389	d__Bacteria;p__Elusimicrobiota;c__Elusimicrobia;o__Elusimicrobiales;f__Elusimicrobiaceae;g__JABHZW01	95.0	99.82	99.62	0.95	0.91	8	conclusive
--------------------------------------------------------------------------------
[2023-03-18 22:17:52,750] [INFO] GTDB search result was written to OceanDNA-b17149/result_gtdb.tsv
[2023-03-18 22:17:52,750] [INFO] ===== GTDB Search completed =====
[2023-03-18 22:17:52,750] [INFO] DFAST_QC result json was written to OceanDNA-b17149/dqc_result.json
[2023-03-18 22:17:52,750] [INFO] DFAST_QC completed!
[2023-03-18 22:17:52,750] [INFO] Total running time: 0h0m40s