[2023-03-18 05:17:48,196] [INFO] DFAST_QC pipeline started.
[2023-03-18 05:17:48,196] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 05:17:48,196] [INFO] DQC Reference Directory: /var/lib/cwl/stg1ee07a4b-e92a-4643-84c9-4f4bcfcfba84/dqc_reference
[2023-03-18 05:17:49,268] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 05:17:49,269] [INFO] Task started: Prodigal
[2023-03-18 05:17:49,269] [INFO] Running command: cat /var/lib/cwl/stgaeb26959-658f-4451-a6ae-81e9fdb7c664/OceanDNA-b17165.fa | prodigal -d OceanDNA-b17165/cds.fna -a OceanDNA-b17165/protein.faa -g 11 -q > /dev/null
[2023-03-18 05:17:53,681] [INFO] Task succeeded: Prodigal
[2023-03-18 05:17:53,681] [INFO] Task started: HMMsearch
[2023-03-18 05:17:53,681] [INFO] Running command: hmmsearch --tblout OceanDNA-b17165/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1ee07a4b-e92a-4643-84c9-4f4bcfcfba84/dqc_reference/reference_markers.hmm OceanDNA-b17165/protein.faa > /dev/null
[2023-03-18 05:17:53,852] [INFO] Task succeeded: HMMsearch
[2023-03-18 05:17:53,853] [INFO] Found 6/6 markers.
[2023-03-18 05:17:53,864] [INFO] Query marker FASTA was written to OceanDNA-b17165/markers.fasta
[2023-03-18 05:17:53,865] [INFO] Task started: Blastn
[2023-03-18 05:17:53,865] [INFO] Running command: blastn -query OceanDNA-b17165/markers.fasta -db /var/lib/cwl/stg1ee07a4b-e92a-4643-84c9-4f4bcfcfba84/dqc_reference/reference_markers.fasta -out OceanDNA-b17165/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 05:17:54,401] [INFO] Task succeeded: Blastn
[2023-03-18 05:17:54,401] [INFO] Selected 20 target genomes.
[2023-03-18 05:17:54,402] [INFO] Target genome list was writen to OceanDNA-b17165/target_genomes.txt
[2023-03-18 05:17:54,422] [INFO] Task started: fastANI
[2023-03-18 05:17:54,422] [INFO] Running command: fastANI --query /var/lib/cwl/stgaeb26959-658f-4451-a6ae-81e9fdb7c664/OceanDNA-b17165.fa --refList OceanDNA-b17165/target_genomes.txt --output OceanDNA-b17165/fastani_result.tsv --threads 1
[2023-03-18 05:18:04,556] [INFO] Task succeeded: fastANI
[2023-03-18 05:18:04,556] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1ee07a4b-e92a-4643-84c9-4f4bcfcfba84/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 05:18:04,556] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1ee07a4b-e92a-4643-84c9-4f4bcfcfba84/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 05:18:04,557] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 05:18:04,557] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 05:18:04,557] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 05:18:04,557] [INFO] DFAST Taxonomy check result was written to OceanDNA-b17165/tc_result.tsv
[2023-03-18 05:18:04,557] [INFO] ===== Taxonomy check completed =====
[2023-03-18 05:18:04,557] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 05:18:04,557] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1ee07a4b-e92a-4643-84c9-4f4bcfcfba84/dqc_reference/checkm_data
[2023-03-18 05:18:04,560] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 05:18:04,567] [INFO] Task started: CheckM
[2023-03-18 05:18:04,567] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b17165/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b17165/checkm_input OceanDNA-b17165/checkm_result
[2023-03-18 05:18:21,843] [INFO] Task succeeded: CheckM
[2023-03-18 05:18:21,844] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 85.76%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 05:18:21,847] [INFO] ===== Completeness check finished =====
[2023-03-18 05:18:21,847] [INFO] ===== Start GTDB Search =====
[2023-03-18 05:18:21,847] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b17165/markers.fasta)
[2023-03-18 05:18:21,848] [INFO] Task started: Blastn
[2023-03-18 05:18:21,848] [INFO] Running command: blastn -query OceanDNA-b17165/markers.fasta -db /var/lib/cwl/stg1ee07a4b-e92a-4643-84c9-4f4bcfcfba84/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b17165/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 05:18:22,657] [INFO] Task succeeded: Blastn
[2023-03-18 05:18:22,658] [INFO] Selected 18 target genomes.
[2023-03-18 05:18:22,658] [INFO] Target genome list was writen to OceanDNA-b17165/target_genomes_gtdb.txt
[2023-03-18 05:18:22,671] [INFO] Task started: fastANI
[2023-03-18 05:18:22,671] [INFO] Running command: fastANI --query /var/lib/cwl/stgaeb26959-658f-4451-a6ae-81e9fdb7c664/OceanDNA-b17165.fa --refList OceanDNA-b17165/target_genomes_gtdb.txt --output OceanDNA-b17165/fastani_result_gtdb.tsv --threads 1
[2023-03-18 05:18:27,434] [INFO] Task succeeded: fastANI
[2023-03-18 05:18:27,436] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 05:18:27,437] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_004195325.1	s__Izemoplasma_B sp004195325	77.7392	72	235	d__Bacteria;p__Firmicutes;c__Bacilli;o__Izemoplasmatales;f__Izemoplasmataceae;g__Izemoplasma_B	95.0	N/A	N/A	N/A	N/A	1	-
GCA_000753575.1	s__Izemoplasma_B sp000753575	77.6711	51	235	d__Bacteria;p__Firmicutes;c__Bacilli;o__Izemoplasmatales;f__Izemoplasmataceae;g__Izemoplasma_B	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 05:18:27,437] [INFO] GTDB search result was written to OceanDNA-b17165/result_gtdb.tsv
[2023-03-18 05:18:27,437] [INFO] ===== GTDB Search completed =====
[2023-03-18 05:18:27,437] [INFO] DFAST_QC result json was written to OceanDNA-b17165/dqc_result.json
[2023-03-18 05:18:27,438] [INFO] DFAST_QC completed!
[2023-03-18 05:18:27,438] [INFO] Total running time: 0h0m39s