[2023-03-18 03:27:00,919] [INFO] DFAST_QC pipeline started.
[2023-03-18 03:27:00,919] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 03:27:00,919] [INFO] DQC Reference Directory: /var/lib/cwl/stg651ae35f-339a-400c-895c-349e70411d57/dqc_reference
[2023-03-18 03:27:02,640] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 03:27:02,640] [INFO] Task started: Prodigal
[2023-03-18 03:27:02,640] [INFO] Running command: cat /var/lib/cwl/stg55b42735-5aa4-4e3c-b345-9bee10333090/OceanDNA-b17714.fa | prodigal -d OceanDNA-b17714/cds.fna -a OceanDNA-b17714/protein.faa -g 11 -q > /dev/null
[2023-03-18 03:27:16,447] [INFO] Task succeeded: Prodigal
[2023-03-18 03:27:16,448] [INFO] Task started: HMMsearch
[2023-03-18 03:27:16,448] [INFO] Running command: hmmsearch --tblout OceanDNA-b17714/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg651ae35f-339a-400c-895c-349e70411d57/dqc_reference/reference_markers.hmm OceanDNA-b17714/protein.faa > /dev/null
[2023-03-18 03:27:16,641] [INFO] Task succeeded: HMMsearch
[2023-03-18 03:27:16,641] [INFO] Found 6/6 markers.
[2023-03-18 03:27:16,663] [INFO] Query marker FASTA was written to OceanDNA-b17714/markers.fasta
[2023-03-18 03:27:16,663] [INFO] Task started: Blastn
[2023-03-18 03:27:16,664] [INFO] Running command: blastn -query OceanDNA-b17714/markers.fasta -db /var/lib/cwl/stg651ae35f-339a-400c-895c-349e70411d57/dqc_reference/reference_markers.fasta -out OceanDNA-b17714/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 03:27:17,196] [INFO] Task succeeded: Blastn
[2023-03-18 03:27:17,197] [INFO] Selected 20 target genomes.
[2023-03-18 03:27:17,197] [INFO] Target genome list was writen to OceanDNA-b17714/target_genomes.txt
[2023-03-18 03:27:17,208] [INFO] Task started: fastANI
[2023-03-18 03:27:17,208] [INFO] Running command: fastANI --query /var/lib/cwl/stg55b42735-5aa4-4e3c-b345-9bee10333090/OceanDNA-b17714.fa --refList OceanDNA-b17714/target_genomes.txt --output OceanDNA-b17714/fastani_result.tsv --threads 1
[2023-03-18 03:27:28,428] [INFO] Task succeeded: fastANI
[2023-03-18 03:27:28,428] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg651ae35f-339a-400c-895c-349e70411d57/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 03:27:28,429] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg651ae35f-339a-400c-895c-349e70411d57/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 03:27:28,429] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 03:27:28,429] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 03:27:28,429] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 03:27:28,429] [INFO] DFAST Taxonomy check result was written to OceanDNA-b17714/tc_result.tsv
[2023-03-18 03:27:28,429] [INFO] ===== Taxonomy check completed =====
[2023-03-18 03:27:28,429] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 03:27:28,429] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg651ae35f-339a-400c-895c-349e70411d57/dqc_reference/checkm_data
[2023-03-18 03:27:28,432] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 03:27:28,437] [INFO] Task started: CheckM
[2023-03-18 03:27:28,437] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b17714/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b17714/checkm_input OceanDNA-b17714/checkm_result
[2023-03-18 03:28:06,046] [INFO] Task succeeded: CheckM
[2023-03-18 03:28:06,046] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.77%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 03:28:06,048] [INFO] ===== Completeness check finished =====
[2023-03-18 03:28:06,049] [INFO] ===== Start GTDB Search =====
[2023-03-18 03:28:06,049] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b17714/markers.fasta)
[2023-03-18 03:28:06,049] [INFO] Task started: Blastn
[2023-03-18 03:28:06,049] [INFO] Running command: blastn -query OceanDNA-b17714/markers.fasta -db /var/lib/cwl/stg651ae35f-339a-400c-895c-349e70411d57/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b17714/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 03:28:06,764] [INFO] Task succeeded: Blastn
[2023-03-18 03:28:06,765] [INFO] Selected 30 target genomes.
[2023-03-18 03:28:06,765] [INFO] Target genome list was writen to OceanDNA-b17714/target_genomes_gtdb.txt
[2023-03-18 03:28:06,795] [INFO] Task started: fastANI
[2023-03-18 03:28:06,795] [INFO] Running command: fastANI --query /var/lib/cwl/stg55b42735-5aa4-4e3c-b345-9bee10333090/OceanDNA-b17714.fa --refList OceanDNA-b17714/target_genomes_gtdb.txt --output OceanDNA-b17714/fastani_result_gtdb.tsv --threads 1
[2023-03-18 03:28:24,883] [INFO] Task succeeded: fastANI
[2023-03-18 03:28:24,886] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 03:28:24,886] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002726335.1	s__UBA8231 sp002726335	75.2105	52	719	d__Bacteria;p__Latescibacterota;c__UBA2968;o__UBA8231;f__UBA8231;g__UBA8231	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 03:28:24,886] [INFO] GTDB search result was written to OceanDNA-b17714/result_gtdb.tsv
[2023-03-18 03:28:24,886] [INFO] ===== GTDB Search completed =====
[2023-03-18 03:28:24,886] [INFO] DFAST_QC result json was written to OceanDNA-b17714/dqc_result.json
[2023-03-18 03:28:24,886] [INFO] DFAST_QC completed!
[2023-03-18 03:28:24,887] [INFO] Total running time: 0h1m24s