[2023-03-16 23:05:03,823] [INFO] DFAST_QC pipeline started.
[2023-03-16 23:05:03,823] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 23:05:03,824] [INFO] DQC Reference Directory: /var/lib/cwl/stg97009bee-9828-4d96-856e-4be150676286/dqc_reference
[2023-03-16 23:05:05,143] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 23:05:05,143] [INFO] Task started: Prodigal
[2023-03-16 23:05:05,143] [INFO] Running command: cat /var/lib/cwl/stg19e2107c-37cf-4bdd-b4d0-a9e3f9a493d8/OceanDNA-b1932.fa | prodigal -d OceanDNA-b1932/cds.fna -a OceanDNA-b1932/protein.faa -g 11 -q > /dev/null
[2023-03-16 23:05:13,441] [INFO] Task succeeded: Prodigal
[2023-03-16 23:05:13,441] [INFO] Task started: HMMsearch
[2023-03-16 23:05:13,441] [INFO] Running command: hmmsearch --tblout OceanDNA-b1932/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg97009bee-9828-4d96-856e-4be150676286/dqc_reference/reference_markers.hmm OceanDNA-b1932/protein.faa > /dev/null
[2023-03-16 23:05:13,609] [INFO] Task succeeded: HMMsearch
[2023-03-16 23:05:13,610] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg19e2107c-37cf-4bdd-b4d0-a9e3f9a493d8/OceanDNA-b1932.fa]
[2023-03-16 23:05:13,621] [INFO] Query marker FASTA was written to OceanDNA-b1932/markers.fasta
[2023-03-16 23:05:13,621] [INFO] Task started: Blastn
[2023-03-16 23:05:13,621] [INFO] Running command: blastn -query OceanDNA-b1932/markers.fasta -db /var/lib/cwl/stg97009bee-9828-4d96-856e-4be150676286/dqc_reference/reference_markers.fasta -out OceanDNA-b1932/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:05:14,106] [INFO] Task succeeded: Blastn
[2023-03-16 23:05:14,106] [INFO] Selected 6 target genomes.
[2023-03-16 23:05:14,106] [INFO] Target genome list was writen to OceanDNA-b1932/target_genomes.txt
[2023-03-16 23:05:14,110] [INFO] Task started: fastANI
[2023-03-16 23:05:14,110] [INFO] Running command: fastANI --query /var/lib/cwl/stg19e2107c-37cf-4bdd-b4d0-a9e3f9a493d8/OceanDNA-b1932.fa --refList OceanDNA-b1932/target_genomes.txt --output OceanDNA-b1932/fastani_result.tsv --threads 1
[2023-03-16 23:05:15,941] [INFO] Task succeeded: fastANI
[2023-03-16 23:05:15,942] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg97009bee-9828-4d96-856e-4be150676286/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 23:05:15,942] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg97009bee-9828-4d96-856e-4be150676286/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 23:05:15,942] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 23:05:15,942] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 23:05:15,942] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 23:05:15,942] [INFO] DFAST Taxonomy check result was written to OceanDNA-b1932/tc_result.tsv
[2023-03-16 23:05:15,942] [INFO] ===== Taxonomy check completed =====
[2023-03-16 23:05:15,943] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 23:05:15,943] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg97009bee-9828-4d96-856e-4be150676286/dqc_reference/checkm_data
[2023-03-16 23:05:15,945] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 23:05:15,949] [INFO] Task started: CheckM
[2023-03-16 23:05:15,949] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b1932/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b1932/checkm_input OceanDNA-b1932/checkm_result
[2023-03-16 23:05:41,839] [INFO] Task succeeded: CheckM
[2023-03-16 23:05:41,839] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 0.52%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-16 23:05:41,841] [INFO] ===== Completeness check finished =====
[2023-03-16 23:05:41,841] [INFO] ===== Start GTDB Search =====
[2023-03-16 23:05:41,842] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b1932/markers.fasta)
[2023-03-16 23:05:41,842] [INFO] Task started: Blastn
[2023-03-16 23:05:41,842] [INFO] Running command: blastn -query OceanDNA-b1932/markers.fasta -db /var/lib/cwl/stg97009bee-9828-4d96-856e-4be150676286/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b1932/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:05:42,494] [INFO] Task succeeded: Blastn
[2023-03-16 23:05:42,495] [INFO] Selected 17 target genomes.
[2023-03-16 23:05:42,495] [INFO] Target genome list was writen to OceanDNA-b1932/target_genomes_gtdb.txt
[2023-03-16 23:05:43,078] [INFO] Task started: fastANI
[2023-03-16 23:05:43,078] [INFO] Running command: fastANI --query /var/lib/cwl/stg19e2107c-37cf-4bdd-b4d0-a9e3f9a493d8/OceanDNA-b1932.fa --refList OceanDNA-b1932/target_genomes_gtdb.txt --output OceanDNA-b1932/fastani_result_gtdb.tsv --threads 1
[2023-03-16 23:05:48,812] [INFO] Task succeeded: fastANI
[2023-03-16 23:05:48,815] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 23:05:48,815] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002709645.1	s__UBA8592 sp002709645	99.7147	416	494	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__UBA8592;g__UBA8592	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_013912105.1	s__UBA8592 sp002171605	77.6566	151	494	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__UBA8592;g__UBA8592	95.0	99.28	98.74	0.85	0.81	5	-
--------------------------------------------------------------------------------
[2023-03-16 23:05:48,815] [INFO] GTDB search result was written to OceanDNA-b1932/result_gtdb.tsv
[2023-03-16 23:05:48,815] [INFO] ===== GTDB Search completed =====
[2023-03-16 23:05:48,816] [INFO] DFAST_QC result json was written to OceanDNA-b1932/dqc_result.json
[2023-03-16 23:05:48,816] [INFO] DFAST_QC completed!
[2023-03-16 23:05:48,816] [INFO] Total running time: 0h0m45s