[2023-03-17 22:46:02,541] [INFO] DFAST_QC pipeline started.
[2023-03-17 22:46:02,541] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 22:46:02,541] [INFO] DQC Reference Directory: /var/lib/cwl/stgf26789ee-b16d-44d8-a8de-0dca0ea71c5d/dqc_reference
[2023-03-17 22:46:03,671] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 22:46:03,672] [INFO] Task started: Prodigal
[2023-03-17 22:46:03,672] [INFO] Running command: cat /var/lib/cwl/stg83ea2242-1b97-4bf2-99a1-5ae4eb4b4137/OceanDNA-b19658.fa | prodigal -d OceanDNA-b19658/cds.fna -a OceanDNA-b19658/protein.faa -g 11 -q > /dev/null
[2023-03-17 22:46:08,440] [INFO] Task succeeded: Prodigal
[2023-03-17 22:46:08,440] [INFO] Task started: HMMsearch
[2023-03-17 22:46:08,441] [INFO] Running command: hmmsearch --tblout OceanDNA-b19658/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf26789ee-b16d-44d8-a8de-0dca0ea71c5d/dqc_reference/reference_markers.hmm OceanDNA-b19658/protein.faa > /dev/null
[2023-03-17 22:46:08,585] [INFO] Task succeeded: HMMsearch
[2023-03-17 22:46:08,585] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg83ea2242-1b97-4bf2-99a1-5ae4eb4b4137/OceanDNA-b19658.fa]
[2023-03-17 22:46:08,601] [INFO] Query marker FASTA was written to OceanDNA-b19658/markers.fasta
[2023-03-17 22:46:08,601] [INFO] Task started: Blastn
[2023-03-17 22:46:08,601] [INFO] Running command: blastn -query OceanDNA-b19658/markers.fasta -db /var/lib/cwl/stgf26789ee-b16d-44d8-a8de-0dca0ea71c5d/dqc_reference/reference_markers.fasta -out OceanDNA-b19658/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 22:46:09,114] [INFO] Task succeeded: Blastn
[2023-03-17 22:46:09,115] [INFO] Selected 17 target genomes.
[2023-03-17 22:46:09,115] [INFO] Target genome list was writen to OceanDNA-b19658/target_genomes.txt
[2023-03-17 22:46:09,126] [INFO] Task started: fastANI
[2023-03-17 22:46:09,127] [INFO] Running command: fastANI --query /var/lib/cwl/stg83ea2242-1b97-4bf2-99a1-5ae4eb4b4137/OceanDNA-b19658.fa --refList OceanDNA-b19658/target_genomes.txt --output OceanDNA-b19658/fastani_result.tsv --threads 1
[2023-03-17 22:46:19,204] [INFO] Task succeeded: fastANI
[2023-03-17 22:46:19,204] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf26789ee-b16d-44d8-a8de-0dca0ea71c5d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 22:46:19,204] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf26789ee-b16d-44d8-a8de-0dca0ea71c5d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 22:46:19,204] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 22:46:19,204] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 22:46:19,204] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 22:46:19,205] [INFO] DFAST Taxonomy check result was written to OceanDNA-b19658/tc_result.tsv
[2023-03-17 22:46:19,205] [INFO] ===== Taxonomy check completed =====
[2023-03-17 22:46:19,205] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 22:46:19,205] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf26789ee-b16d-44d8-a8de-0dca0ea71c5d/dqc_reference/checkm_data
[2023-03-17 22:46:19,208] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 22:46:19,281] [INFO] Task started: CheckM
[2023-03-17 22:46:19,281] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b19658/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b19658/checkm_input OceanDNA-b19658/checkm_result
[2023-03-17 22:46:37,442] [INFO] Task succeeded: CheckM
[2023-03-17 22:46:37,442] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 74.64%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-17 22:46:37,444] [INFO] ===== Completeness check finished =====
[2023-03-17 22:46:37,444] [INFO] ===== Start GTDB Search =====
[2023-03-17 22:46:37,444] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b19658/markers.fasta)
[2023-03-17 22:46:37,445] [INFO] Task started: Blastn
[2023-03-17 22:46:37,445] [INFO] Running command: blastn -query OceanDNA-b19658/markers.fasta -db /var/lib/cwl/stgf26789ee-b16d-44d8-a8de-0dca0ea71c5d/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b19658/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 22:46:38,096] [INFO] Task succeeded: Blastn
[2023-03-17 22:46:38,097] [INFO] Selected 16 target genomes.
[2023-03-17 22:46:38,097] [INFO] Target genome list was writen to OceanDNA-b19658/target_genomes_gtdb.txt
[2023-03-17 22:46:38,111] [INFO] Task started: fastANI
[2023-03-17 22:46:38,111] [INFO] Running command: fastANI --query /var/lib/cwl/stg83ea2242-1b97-4bf2-99a1-5ae4eb4b4137/OceanDNA-b19658.fa --refList OceanDNA-b19658/target_genomes_gtdb.txt --output OceanDNA-b19658/fastani_result_gtdb.tsv --threads 1
[2023-03-17 22:46:43,959] [INFO] Task succeeded: fastANI
[2023-03-17 22:46:43,962] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-17 22:46:43,962] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002167345.2	s__CACEUX01 sp002167345	98.6567	201	208	d__Bacteria;p__Marinisomatota;c__Marinisomatia;o__Marinisomatales;f__UBA1611;g__CACEUX01	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_902562835.1	s__CACEUX01 sp902562835	94.0779	154	208	d__Bacteria;p__Marinisomatota;c__Marinisomatia;o__Marinisomatales;f__UBA1611;g__CACEUX01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-17 22:46:43,962] [INFO] GTDB search result was written to OceanDNA-b19658/result_gtdb.tsv
[2023-03-17 22:46:43,962] [INFO] ===== GTDB Search completed =====
[2023-03-17 22:46:43,963] [INFO] DFAST_QC result json was written to OceanDNA-b19658/dqc_result.json
[2023-03-17 22:46:43,963] [INFO] DFAST_QC completed!
[2023-03-17 22:46:43,963] [INFO] Total running time: 0h0m41s