[2023-03-18 07:06:19,997] [INFO] DFAST_QC pipeline started.
[2023-03-18 07:06:19,997] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 07:06:19,997] [INFO] DQC Reference Directory: /var/lib/cwl/stgfe633916-2d9f-4394-83c9-6ca75a244fa5/dqc_reference
[2023-03-18 07:06:21,100] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 07:06:21,101] [INFO] Task started: Prodigal
[2023-03-18 07:06:21,101] [INFO] Running command: cat /var/lib/cwl/stg40ba2a21-e5ee-467f-865a-3ffcb45cf5d6/OceanDNA-b20104.fa | prodigal -d OceanDNA-b20104/cds.fna -a OceanDNA-b20104/protein.faa -g 11 -q > /dev/null
[2023-03-18 07:06:25,453] [INFO] Task succeeded: Prodigal
[2023-03-18 07:06:25,453] [INFO] Task started: HMMsearch
[2023-03-18 07:06:25,453] [INFO] Running command: hmmsearch --tblout OceanDNA-b20104/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfe633916-2d9f-4394-83c9-6ca75a244fa5/dqc_reference/reference_markers.hmm OceanDNA-b20104/protein.faa > /dev/null
[2023-03-18 07:06:25,600] [INFO] Task succeeded: HMMsearch
[2023-03-18 07:06:25,601] [INFO] Found 6/6 markers.
[2023-03-18 07:06:25,615] [INFO] Query marker FASTA was written to OceanDNA-b20104/markers.fasta
[2023-03-18 07:06:25,615] [INFO] Task started: Blastn
[2023-03-18 07:06:25,615] [INFO] Running command: blastn -query OceanDNA-b20104/markers.fasta -db /var/lib/cwl/stgfe633916-2d9f-4394-83c9-6ca75a244fa5/dqc_reference/reference_markers.fasta -out OceanDNA-b20104/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 07:06:26,111] [INFO] Task succeeded: Blastn
[2023-03-18 07:06:26,111] [INFO] Selected 20 target genomes.
[2023-03-18 07:06:26,112] [INFO] Target genome list was writen to OceanDNA-b20104/target_genomes.txt
[2023-03-18 07:06:26,123] [INFO] Task started: fastANI
[2023-03-18 07:06:26,123] [INFO] Running command: fastANI --query /var/lib/cwl/stg40ba2a21-e5ee-467f-865a-3ffcb45cf5d6/OceanDNA-b20104.fa --refList OceanDNA-b20104/target_genomes.txt --output OceanDNA-b20104/fastani_result.tsv --threads 1
[2023-03-18 07:06:34,105] [INFO] Task succeeded: fastANI
[2023-03-18 07:06:34,105] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgfe633916-2d9f-4394-83c9-6ca75a244fa5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 07:06:34,106] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgfe633916-2d9f-4394-83c9-6ca75a244fa5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 07:06:34,106] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 07:06:34,106] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 07:06:34,106] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 07:06:34,106] [INFO] DFAST Taxonomy check result was written to OceanDNA-b20104/tc_result.tsv
[2023-03-18 07:06:34,106] [INFO] ===== Taxonomy check completed =====
[2023-03-18 07:06:34,106] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 07:06:34,106] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfe633916-2d9f-4394-83c9-6ca75a244fa5/dqc_reference/checkm_data
[2023-03-18 07:06:34,109] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 07:06:34,117] [INFO] Task started: CheckM
[2023-03-18 07:06:34,117] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b20104/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b20104/checkm_input OceanDNA-b20104/checkm_result
[2023-03-18 07:06:51,973] [INFO] Task succeeded: CheckM
[2023-03-18 07:06:51,974] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.30%
Contamintation: 4.69%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-18 07:06:51,975] [INFO] ===== Completeness check finished =====
[2023-03-18 07:06:51,976] [INFO] ===== Start GTDB Search =====
[2023-03-18 07:06:51,976] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b20104/markers.fasta)
[2023-03-18 07:06:51,976] [INFO] Task started: Blastn
[2023-03-18 07:06:51,976] [INFO] Running command: blastn -query OceanDNA-b20104/markers.fasta -db /var/lib/cwl/stgfe633916-2d9f-4394-83c9-6ca75a244fa5/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b20104/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 07:06:52,688] [INFO] Task succeeded: Blastn
[2023-03-18 07:06:52,688] [INFO] Selected 24 target genomes.
[2023-03-18 07:06:52,688] [INFO] Target genome list was writen to OceanDNA-b20104/target_genomes_gtdb.txt
[2023-03-18 07:06:53,297] [INFO] Task started: fastANI
[2023-03-18 07:06:53,297] [INFO] Running command: fastANI --query /var/lib/cwl/stg40ba2a21-e5ee-467f-865a-3ffcb45cf5d6/OceanDNA-b20104.fa --refList OceanDNA-b20104/target_genomes_gtdb.txt --output OceanDNA-b20104/fastani_result_gtdb.tsv --threads 1
[2023-03-18 07:07:01,411] [INFO] Task succeeded: fastANI
[2023-03-18 07:07:01,412] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 07:07:01,413] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_004321745.1	s__TMED80 sp002170405	90.436	88	140	d__Bacteria;p__Marinisomatota;c__Marinisomatia;o__Marinisomatales;f__UBA1611;g__TMED80	95.0	99.32	99.32	0.73	0.73	2	-
--------------------------------------------------------------------------------
[2023-03-18 07:07:01,413] [INFO] GTDB search result was written to OceanDNA-b20104/result_gtdb.tsv
[2023-03-18 07:07:01,413] [INFO] ===== GTDB Search completed =====
[2023-03-18 07:07:01,413] [INFO] DFAST_QC result json was written to OceanDNA-b20104/dqc_result.json
[2023-03-18 07:07:01,413] [INFO] DFAST_QC completed!
[2023-03-18 07:07:01,413] [INFO] Total running time: 0h0m41s