[2023-03-16 21:45:15,278] [INFO] DFAST_QC pipeline started.
[2023-03-16 21:45:15,278] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 21:45:15,279] [INFO] DQC Reference Directory: /var/lib/cwl/stg9795560b-0ef8-4638-af14-664d3d6c4807/dqc_reference
[2023-03-16 21:45:16,453] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 21:45:16,453] [INFO] Task started: Prodigal
[2023-03-16 21:45:16,453] [INFO] Running command: cat /var/lib/cwl/stgecfcbfca-1fa1-4b03-8324-8299eba14ea8/OceanDNA-b20486.fa | prodigal -d OceanDNA-b20486/cds.fna -a OceanDNA-b20486/protein.faa -g 11 -q > /dev/null
[2023-03-16 21:45:23,321] [INFO] Task succeeded: Prodigal
[2023-03-16 21:45:23,321] [INFO] Task started: HMMsearch
[2023-03-16 21:45:23,321] [INFO] Running command: hmmsearch --tblout OceanDNA-b20486/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9795560b-0ef8-4638-af14-664d3d6c4807/dqc_reference/reference_markers.hmm OceanDNA-b20486/protein.faa > /dev/null
[2023-03-16 21:45:23,532] [INFO] Task succeeded: HMMsearch
[2023-03-16 21:45:23,533] [INFO] Found 6/6 markers.
[2023-03-16 21:45:23,544] [INFO] Query marker FASTA was written to OceanDNA-b20486/markers.fasta
[2023-03-16 21:45:23,544] [INFO] Task started: Blastn
[2023-03-16 21:45:23,544] [INFO] Running command: blastn -query OceanDNA-b20486/markers.fasta -db /var/lib/cwl/stg9795560b-0ef8-4638-af14-664d3d6c4807/dqc_reference/reference_markers.fasta -out OceanDNA-b20486/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 21:45:24,082] [INFO] Task succeeded: Blastn
[2023-03-16 21:45:24,082] [INFO] Selected 18 target genomes.
[2023-03-16 21:45:24,083] [INFO] Target genome list was writen to OceanDNA-b20486/target_genomes.txt
[2023-03-16 21:45:24,092] [INFO] Task started: fastANI
[2023-03-16 21:45:24,092] [INFO] Running command: fastANI --query /var/lib/cwl/stgecfcbfca-1fa1-4b03-8324-8299eba14ea8/OceanDNA-b20486.fa --refList OceanDNA-b20486/target_genomes.txt --output OceanDNA-b20486/fastani_result.tsv --threads 1
[2023-03-16 21:45:32,458] [INFO] Task succeeded: fastANI
[2023-03-16 21:45:32,459] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9795560b-0ef8-4638-af14-664d3d6c4807/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 21:45:32,459] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9795560b-0ef8-4638-af14-664d3d6c4807/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 21:45:32,459] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 21:45:32,459] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 21:45:32,459] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 21:45:32,460] [INFO] DFAST Taxonomy check result was written to OceanDNA-b20486/tc_result.tsv
[2023-03-16 21:45:32,460] [INFO] ===== Taxonomy check completed =====
[2023-03-16 21:45:32,460] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 21:45:32,460] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9795560b-0ef8-4638-af14-664d3d6c4807/dqc_reference/checkm_data
[2023-03-16 21:45:32,462] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 21:45:32,465] [INFO] Task started: CheckM
[2023-03-16 21:45:32,465] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b20486/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b20486/checkm_input OceanDNA-b20486/checkm_result
[2023-03-16 21:45:55,174] [INFO] Task succeeded: CheckM
[2023-03-16 21:45:55,175] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.08%
Contamintation: 0.46%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-16 21:45:55,177] [INFO] ===== Completeness check finished =====
[2023-03-16 21:45:55,177] [INFO] ===== Start GTDB Search =====
[2023-03-16 21:45:55,177] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b20486/markers.fasta)
[2023-03-16 21:45:55,177] [INFO] Task started: Blastn
[2023-03-16 21:45:55,177] [INFO] Running command: blastn -query OceanDNA-b20486/markers.fasta -db /var/lib/cwl/stg9795560b-0ef8-4638-af14-664d3d6c4807/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b20486/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 21:45:55,906] [INFO] Task succeeded: Blastn
[2023-03-16 21:45:55,907] [INFO] Selected 24 target genomes.
[2023-03-16 21:45:55,907] [INFO] Target genome list was writen to OceanDNA-b20486/target_genomes_gtdb.txt
[2023-03-16 21:45:55,937] [INFO] Task started: fastANI
[2023-03-16 21:45:55,937] [INFO] Running command: fastANI --query /var/lib/cwl/stgecfcbfca-1fa1-4b03-8324-8299eba14ea8/OceanDNA-b20486.fa --refList OceanDNA-b20486/target_genomes_gtdb.txt --output OceanDNA-b20486/fastani_result_gtdb.tsv --threads 1
[2023-03-16 21:46:02,928] [INFO] Task succeeded: fastANI
[2023-03-16 21:46:02,931] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 21:46:02,931] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002704045.1	s__GCA-002704045 sp002704045	86.5089	297	463	d__Bacteria;p__Marinisomatota;c__Marinisomatia;o__SCGC-AAA003-L08;f__GCA-002704045;g__GCA-002704045	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002703375.1	s__GCA-002704045 sp002703375	86.278	385	463	d__Bacteria;p__Marinisomatota;c__Marinisomatia;o__SCGC-AAA003-L08;f__GCA-002704045;g__GCA-002704045	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 21:46:02,931] [INFO] GTDB search result was written to OceanDNA-b20486/result_gtdb.tsv
[2023-03-16 21:46:02,931] [INFO] ===== GTDB Search completed =====
[2023-03-16 21:46:02,932] [INFO] DFAST_QC result json was written to OceanDNA-b20486/dqc_result.json
[2023-03-16 21:46:02,932] [INFO] DFAST_QC completed!
[2023-03-16 21:46:02,932] [INFO] Total running time: 0h0m48s