[2023-03-15 08:55:06,628] [INFO] DFAST_QC pipeline started.
[2023-03-15 08:55:06,628] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 08:55:06,628] [INFO] DQC Reference Directory: /var/lib/cwl/stg493f35e9-fdd3-4f66-9a40-9298ae8a7bc5/dqc_reference
[2023-03-15 08:55:08,346] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 08:55:08,346] [INFO] Task started: Prodigal
[2023-03-15 08:55:08,346] [INFO] Running command: cat /var/lib/cwl/stgb622312b-1327-4931-bde6-f363f37575f2/OceanDNA-b20595.fa | prodigal -d OceanDNA-b20595/cds.fna -a OceanDNA-b20595/protein.faa -g 11 -q > /dev/null
[2023-03-15 08:55:14,683] [INFO] Task succeeded: Prodigal
[2023-03-15 08:55:14,683] [INFO] Task started: HMMsearch
[2023-03-15 08:55:14,683] [INFO] Running command: hmmsearch --tblout OceanDNA-b20595/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg493f35e9-fdd3-4f66-9a40-9298ae8a7bc5/dqc_reference/reference_markers.hmm OceanDNA-b20595/protein.faa > /dev/null
[2023-03-15 08:55:14,884] [INFO] Task succeeded: HMMsearch
[2023-03-15 08:55:14,885] [INFO] Found 6/6 markers.
[2023-03-15 08:55:14,894] [INFO] Query marker FASTA was written to OceanDNA-b20595/markers.fasta
[2023-03-15 08:55:14,895] [INFO] Task started: Blastn
[2023-03-15 08:55:14,895] [INFO] Running command: blastn -query OceanDNA-b20595/markers.fasta -db /var/lib/cwl/stg493f35e9-fdd3-4f66-9a40-9298ae8a7bc5/dqc_reference/reference_markers.fasta -out OceanDNA-b20595/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 08:55:15,452] [INFO] Task succeeded: Blastn
[2023-03-15 08:55:15,452] [INFO] Selected 19 target genomes.
[2023-03-15 08:55:15,453] [INFO] Target genome list was writen to OceanDNA-b20595/target_genomes.txt
[2023-03-15 08:55:15,462] [INFO] Task started: fastANI
[2023-03-15 08:55:15,477] [INFO] Running command: fastANI --query /var/lib/cwl/stgb622312b-1327-4931-bde6-f363f37575f2/OceanDNA-b20595.fa --refList OceanDNA-b20595/target_genomes.txt --output OceanDNA-b20595/fastani_result.tsv --threads 1
[2023-03-15 08:55:23,203] [INFO] Task succeeded: fastANI
[2023-03-15 08:55:23,204] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg493f35e9-fdd3-4f66-9a40-9298ae8a7bc5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 08:55:23,204] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg493f35e9-fdd3-4f66-9a40-9298ae8a7bc5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 08:55:23,204] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 08:55:23,204] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 08:55:23,204] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 08:55:23,205] [INFO] DFAST Taxonomy check result was written to OceanDNA-b20595/tc_result.tsv
[2023-03-15 08:55:23,205] [INFO] ===== Taxonomy check completed =====
[2023-03-15 08:55:23,205] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 08:55:23,205] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg493f35e9-fdd3-4f66-9a40-9298ae8a7bc5/dqc_reference/checkm_data
[2023-03-15 08:55:23,208] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 08:55:23,210] [INFO] Task started: CheckM
[2023-03-15 08:55:23,211] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b20595/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b20595/checkm_input OceanDNA-b20595/checkm_result
[2023-03-15 08:55:44,914] [INFO] Task succeeded: CheckM
[2023-03-15 08:55:44,915] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 2.46%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 08:55:44,916] [INFO] ===== Completeness check finished =====
[2023-03-15 08:55:44,916] [INFO] ===== Start GTDB Search =====
[2023-03-15 08:55:44,917] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b20595/markers.fasta)
[2023-03-15 08:55:44,917] [INFO] Task started: Blastn
[2023-03-15 08:55:44,917] [INFO] Running command: blastn -query OceanDNA-b20595/markers.fasta -db /var/lib/cwl/stg493f35e9-fdd3-4f66-9a40-9298ae8a7bc5/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b20595/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 08:55:45,858] [INFO] Task succeeded: Blastn
[2023-03-15 08:55:45,859] [INFO] Selected 26 target genomes.
[2023-03-15 08:55:45,859] [INFO] Target genome list was writen to OceanDNA-b20595/target_genomes_gtdb.txt
[2023-03-15 08:55:46,086] [INFO] Task started: fastANI
[2023-03-15 08:55:46,086] [INFO] Running command: fastANI --query /var/lib/cwl/stgb622312b-1327-4931-bde6-f363f37575f2/OceanDNA-b20595.fa --refList OceanDNA-b20595/target_genomes_gtdb.txt --output OceanDNA-b20595/fastani_result_gtdb.tsv --threads 1
[2023-03-15 08:55:54,938] [INFO] Task succeeded: fastANI
[2023-03-15 08:55:54,940] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 08:55:54,940] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002719095.1	s__GCA-002719095 sp002719095	99.014	366	408	d__Bacteria;p__Marinisomatota;c__Marinisomatia;o__SCGC-AAA003-L08;f__GCA-002719095;g__GCA-002719095	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002705575.1	s__GCA-002719095 sp002705575	76.0561	63	408	d__Bacteria;p__Marinisomatota;c__Marinisomatia;o__SCGC-AAA003-L08;f__GCA-002719095;g__GCA-002719095	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 08:55:54,941] [INFO] GTDB search result was written to OceanDNA-b20595/result_gtdb.tsv
[2023-03-15 08:55:54,941] [INFO] ===== GTDB Search completed =====
[2023-03-15 08:55:54,941] [INFO] DFAST_QC result json was written to OceanDNA-b20595/dqc_result.json
[2023-03-15 08:55:54,941] [INFO] DFAST_QC completed!
[2023-03-15 08:55:54,941] [INFO] Total running time: 0h0m48s