[2023-03-16 21:45:13,524] [INFO] DFAST_QC pipeline started.
[2023-03-16 21:45:13,524] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 21:45:13,525] [INFO] DQC Reference Directory: /var/lib/cwl/stg119e0d9a-4e89-4713-843a-113179836e80/dqc_reference
[2023-03-16 21:45:15,326] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 21:45:15,327] [INFO] Task started: Prodigal
[2023-03-16 21:45:15,327] [INFO] Running command: cat /var/lib/cwl/stgfa3c57d7-4862-493b-abf8-df25bbfc2dfc/OceanDNA-b20941.fa | prodigal -d OceanDNA-b20941/cds.fna -a OceanDNA-b20941/protein.faa -g 11 -q > /dev/null
[2023-03-16 21:45:36,226] [INFO] Task succeeded: Prodigal
[2023-03-16 21:45:36,226] [INFO] Task started: HMMsearch
[2023-03-16 21:45:36,226] [INFO] Running command: hmmsearch --tblout OceanDNA-b20941/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg119e0d9a-4e89-4713-843a-113179836e80/dqc_reference/reference_markers.hmm OceanDNA-b20941/protein.faa > /dev/null
[2023-03-16 21:45:36,476] [INFO] Task succeeded: HMMsearch
[2023-03-16 21:45:36,477] [INFO] Found 6/6 markers.
[2023-03-16 21:45:36,500] [INFO] Query marker FASTA was written to OceanDNA-b20941/markers.fasta
[2023-03-16 21:45:36,500] [INFO] Task started: Blastn
[2023-03-16 21:45:36,500] [INFO] Running command: blastn -query OceanDNA-b20941/markers.fasta -db /var/lib/cwl/stg119e0d9a-4e89-4713-843a-113179836e80/dqc_reference/reference_markers.fasta -out OceanDNA-b20941/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 21:45:37,044] [INFO] Task succeeded: Blastn
[2023-03-16 21:45:37,045] [INFO] Selected 25 target genomes.
[2023-03-16 21:45:37,045] [INFO] Target genome list was writen to OceanDNA-b20941/target_genomes.txt
[2023-03-16 21:45:37,060] [INFO] Task started: fastANI
[2023-03-16 21:45:37,060] [INFO] Running command: fastANI --query /var/lib/cwl/stgfa3c57d7-4862-493b-abf8-df25bbfc2dfc/OceanDNA-b20941.fa --refList OceanDNA-b20941/target_genomes.txt --output OceanDNA-b20941/fastani_result.tsv --threads 1
[2023-03-16 21:45:53,845] [INFO] Task succeeded: fastANI
[2023-03-16 21:45:53,845] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg119e0d9a-4e89-4713-843a-113179836e80/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 21:45:53,845] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg119e0d9a-4e89-4713-843a-113179836e80/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 21:45:53,846] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 21:45:53,846] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 21:45:53,846] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 21:45:53,846] [INFO] DFAST Taxonomy check result was written to OceanDNA-b20941/tc_result.tsv
[2023-03-16 21:45:53,846] [INFO] ===== Taxonomy check completed =====
[2023-03-16 21:45:53,846] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 21:45:53,846] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg119e0d9a-4e89-4713-843a-113179836e80/dqc_reference/checkm_data
[2023-03-16 21:45:53,849] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 21:45:53,855] [INFO] Task started: CheckM
[2023-03-16 21:45:53,856] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b20941/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b20941/checkm_input OceanDNA-b20941/checkm_result
[2023-03-16 21:46:46,395] [INFO] Task succeeded: CheckM
[2023-03-16 21:46:46,395] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.95%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 21:46:46,398] [INFO] ===== Completeness check finished =====
[2023-03-16 21:46:46,398] [INFO] ===== Start GTDB Search =====
[2023-03-16 21:46:46,398] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b20941/markers.fasta)
[2023-03-16 21:46:46,398] [INFO] Task started: Blastn
[2023-03-16 21:46:46,398] [INFO] Running command: blastn -query OceanDNA-b20941/markers.fasta -db /var/lib/cwl/stg119e0d9a-4e89-4713-843a-113179836e80/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b20941/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 21:46:47,176] [INFO] Task succeeded: Blastn
[2023-03-16 21:46:47,176] [INFO] Selected 19 target genomes.
[2023-03-16 21:46:47,177] [INFO] Target genome list was writen to OceanDNA-b20941/target_genomes_gtdb.txt
[2023-03-16 21:46:47,243] [INFO] Task started: fastANI
[2023-03-16 21:46:47,243] [INFO] Running command: fastANI --query /var/lib/cwl/stgfa3c57d7-4862-493b-abf8-df25bbfc2dfc/OceanDNA-b20941.fa --refList OceanDNA-b20941/target_genomes_gtdb.txt --output OceanDNA-b20941/fastani_result_gtdb.tsv --threads 1
[2023-03-16 21:46:58,711] [INFO] Task succeeded: fastANI
[2023-03-16 21:46:58,714] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 21:46:58,714] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002722845.1	s__UBA4151 sp002722845	99.7926	766	1095	d__Bacteria;p__Myxococcota;c__UBA4151;o__UBA4151;f__UBA4151;g__UBA4151	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002696345.1	s__UBA4151 sp002696345	79.015	285	1095	d__Bacteria;p__Myxococcota;c__UBA4151;o__UBA4151;f__UBA4151;g__UBA4151	95.0	98.21	97.58	0.88	0.85	4	-
--------------------------------------------------------------------------------
[2023-03-16 21:46:58,714] [INFO] GTDB search result was written to OceanDNA-b20941/result_gtdb.tsv
[2023-03-16 21:46:58,714] [INFO] ===== GTDB Search completed =====
[2023-03-16 21:46:58,715] [INFO] DFAST_QC result json was written to OceanDNA-b20941/dqc_result.json
[2023-03-16 21:46:58,715] [INFO] DFAST_QC completed!
[2023-03-16 21:46:58,715] [INFO] Total running time: 0h1m45s