[2023-03-15 18:20:15,199] [INFO] DFAST_QC pipeline started.
[2023-03-15 18:20:15,199] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 18:20:15,199] [INFO] DQC Reference Directory: /var/lib/cwl/stgf3157932-2a96-471b-83a8-c59bbdef2205/dqc_reference
[2023-03-15 18:20:16,845] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 18:20:16,845] [INFO] Task started: Prodigal
[2023-03-15 18:20:16,845] [INFO] Running command: cat /var/lib/cwl/stg7ee41890-dceb-469b-80a6-6b6920225372/OceanDNA-b20992.fa | prodigal -d OceanDNA-b20992/cds.fna -a OceanDNA-b20992/protein.faa -g 11 -q > /dev/null
[2023-03-15 18:20:39,820] [INFO] Task succeeded: Prodigal
[2023-03-15 18:20:39,821] [INFO] Task started: HMMsearch
[2023-03-15 18:20:39,821] [INFO] Running command: hmmsearch --tblout OceanDNA-b20992/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf3157932-2a96-471b-83a8-c59bbdef2205/dqc_reference/reference_markers.hmm OceanDNA-b20992/protein.faa > /dev/null
[2023-03-15 18:20:40,080] [INFO] Task succeeded: HMMsearch
[2023-03-15 18:20:40,081] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg7ee41890-dceb-469b-80a6-6b6920225372/OceanDNA-b20992.fa]
[2023-03-15 18:20:40,104] [INFO] Query marker FASTA was written to OceanDNA-b20992/markers.fasta
[2023-03-15 18:20:40,105] [INFO] Task started: Blastn
[2023-03-15 18:20:40,105] [INFO] Running command: blastn -query OceanDNA-b20992/markers.fasta -db /var/lib/cwl/stgf3157932-2a96-471b-83a8-c59bbdef2205/dqc_reference/reference_markers.fasta -out OceanDNA-b20992/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 18:20:40,615] [INFO] Task succeeded: Blastn
[2023-03-15 18:20:40,616] [INFO] Selected 22 target genomes.
[2023-03-15 18:20:40,616] [INFO] Target genome list was writen to OceanDNA-b20992/target_genomes.txt
[2023-03-15 18:20:40,873] [INFO] Task started: fastANI
[2023-03-15 18:20:40,873] [INFO] Running command: fastANI --query /var/lib/cwl/stg7ee41890-dceb-469b-80a6-6b6920225372/OceanDNA-b20992.fa --refList OceanDNA-b20992/target_genomes.txt --output OceanDNA-b20992/fastani_result.tsv --threads 1
[2023-03-15 18:20:54,445] [INFO] Task succeeded: fastANI
[2023-03-15 18:20:54,446] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf3157932-2a96-471b-83a8-c59bbdef2205/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 18:20:54,446] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf3157932-2a96-471b-83a8-c59bbdef2205/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 18:20:54,446] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 18:20:54,446] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 18:20:54,446] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 18:20:54,446] [INFO] DFAST Taxonomy check result was written to OceanDNA-b20992/tc_result.tsv
[2023-03-15 18:20:54,446] [INFO] ===== Taxonomy check completed =====
[2023-03-15 18:20:54,447] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 18:20:54,447] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf3157932-2a96-471b-83a8-c59bbdef2205/dqc_reference/checkm_data
[2023-03-15 18:20:54,449] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 18:20:54,454] [INFO] Task started: CheckM
[2023-03-15 18:20:54,455] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b20992/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b20992/checkm_input OceanDNA-b20992/checkm_result
[2023-03-15 18:21:51,340] [INFO] Task succeeded: CheckM
[2023-03-15 18:21:51,341] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 18:21:51,343] [INFO] ===== Completeness check finished =====
[2023-03-15 18:21:51,343] [INFO] ===== Start GTDB Search =====
[2023-03-15 18:21:51,343] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b20992/markers.fasta)
[2023-03-15 18:21:51,344] [INFO] Task started: Blastn
[2023-03-15 18:21:51,344] [INFO] Running command: blastn -query OceanDNA-b20992/markers.fasta -db /var/lib/cwl/stgf3157932-2a96-471b-83a8-c59bbdef2205/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b20992/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 18:21:52,047] [INFO] Task succeeded: Blastn
[2023-03-15 18:21:52,048] [INFO] Selected 22 target genomes.
[2023-03-15 18:21:52,048] [INFO] Target genome list was writen to OceanDNA-b20992/target_genomes_gtdb.txt
[2023-03-15 18:21:52,125] [INFO] Task started: fastANI
[2023-03-15 18:21:52,125] [INFO] Running command: fastANI --query /var/lib/cwl/stg7ee41890-dceb-469b-80a6-6b6920225372/OceanDNA-b20992.fa --refList OceanDNA-b20992/target_genomes_gtdb.txt --output OceanDNA-b20992/fastani_result_gtdb.tsv --threads 1
[2023-03-15 18:22:04,036] [INFO] Task succeeded: fastANI
[2023-03-15 18:22:04,038] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 18:22:04,039] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002728755.1	s__UBA3505 sp002728755	99.9258	1155	1243	d__Bacteria;p__Myxococcota;c__UBA9042;o__UBA3505;f__UBA3505;g__UBA3505	95.0	99.81	99.81	0.85	0.85	2	conclusive
--------------------------------------------------------------------------------
[2023-03-15 18:22:04,039] [INFO] GTDB search result was written to OceanDNA-b20992/result_gtdb.tsv
[2023-03-15 18:22:04,039] [INFO] ===== GTDB Search completed =====
[2023-03-15 18:22:04,039] [INFO] DFAST_QC result json was written to OceanDNA-b20992/dqc_result.json
[2023-03-15 18:22:04,039] [INFO] DFAST_QC completed!
[2023-03-15 18:22:04,039] [INFO] Total running time: 0h1m49s