[2023-03-16 09:13:21,142] [INFO] DFAST_QC pipeline started.
[2023-03-16 09:13:21,142] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 09:13:21,142] [INFO] DQC Reference Directory: /var/lib/cwl/stged5110c9-db18-408a-a7f1-ace78a5b7b92/dqc_reference
[2023-03-16 09:13:22,472] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 09:13:22,472] [INFO] Task started: Prodigal
[2023-03-16 09:13:22,473] [INFO] Running command: cat /var/lib/cwl/stgacd5f22d-9cb5-4540-bd37-b0ba74c984a0/OceanDNA-b21444.fa | prodigal -d OceanDNA-b21444/cds.fna -a OceanDNA-b21444/protein.faa -g 11 -q > /dev/null
[2023-03-16 09:13:28,162] [INFO] Task succeeded: Prodigal
[2023-03-16 09:13:28,162] [INFO] Task started: HMMsearch
[2023-03-16 09:13:28,162] [INFO] Running command: hmmsearch --tblout OceanDNA-b21444/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stged5110c9-db18-408a-a7f1-ace78a5b7b92/dqc_reference/reference_markers.hmm OceanDNA-b21444/protein.faa > /dev/null
[2023-03-16 09:13:28,331] [INFO] Task succeeded: HMMsearch
[2023-03-16 09:13:28,332] [INFO] Found 6/6 markers.
[2023-03-16 09:13:28,344] [INFO] Query marker FASTA was written to OceanDNA-b21444/markers.fasta
[2023-03-16 09:13:28,345] [INFO] Task started: Blastn
[2023-03-16 09:13:28,345] [INFO] Running command: blastn -query OceanDNA-b21444/markers.fasta -db /var/lib/cwl/stged5110c9-db18-408a-a7f1-ace78a5b7b92/dqc_reference/reference_markers.fasta -out OceanDNA-b21444/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 09:13:29,059] [INFO] Task succeeded: Blastn
[2023-03-16 09:13:29,062] [INFO] Selected 13 target genomes.
[2023-03-16 09:13:29,062] [INFO] Target genome list was writen to OceanDNA-b21444/target_genomes.txt
[2023-03-16 09:13:29,068] [INFO] Task started: fastANI
[2023-03-16 09:13:29,068] [INFO] Running command: fastANI --query /var/lib/cwl/stgacd5f22d-9cb5-4540-bd37-b0ba74c984a0/OceanDNA-b21444.fa --refList OceanDNA-b21444/target_genomes.txt --output OceanDNA-b21444/fastani_result.tsv --threads 1
[2023-03-16 09:13:36,357] [INFO] Task succeeded: fastANI
[2023-03-16 09:13:36,357] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stged5110c9-db18-408a-a7f1-ace78a5b7b92/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 09:13:36,357] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stged5110c9-db18-408a-a7f1-ace78a5b7b92/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 09:13:36,357] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 09:13:36,357] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 09:13:36,357] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 09:13:36,359] [INFO] DFAST Taxonomy check result was written to OceanDNA-b21444/tc_result.tsv
[2023-03-16 09:13:36,361] [INFO] ===== Taxonomy check completed =====
[2023-03-16 09:13:36,361] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 09:13:36,362] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stged5110c9-db18-408a-a7f1-ace78a5b7b92/dqc_reference/checkm_data
[2023-03-16 09:13:36,364] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 09:13:36,372] [INFO] Task started: CheckM
[2023-03-16 09:13:36,372] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b21444/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b21444/checkm_input OceanDNA-b21444/checkm_result
[2023-03-16 09:13:59,105] [INFO] Task succeeded: CheckM
[2023-03-16 09:13:59,105] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.25%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 09:13:59,149] [INFO] ===== Completeness check finished =====
[2023-03-16 09:13:59,149] [INFO] ===== Start GTDB Search =====
[2023-03-16 09:13:59,149] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b21444/markers.fasta)
[2023-03-16 09:13:59,151] [INFO] Task started: Blastn
[2023-03-16 09:13:59,151] [INFO] Running command: blastn -query OceanDNA-b21444/markers.fasta -db /var/lib/cwl/stged5110c9-db18-408a-a7f1-ace78a5b7b92/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b21444/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 09:14:00,235] [INFO] Task succeeded: Blastn
[2023-03-16 09:14:00,237] [INFO] Selected 13 target genomes.
[2023-03-16 09:14:00,238] [INFO] Target genome list was writen to OceanDNA-b21444/target_genomes_gtdb.txt
[2023-03-16 09:14:00,341] [INFO] Task started: fastANI
[2023-03-16 09:14:00,342] [INFO] Running command: fastANI --query /var/lib/cwl/stgacd5f22d-9cb5-4540-bd37-b0ba74c984a0/OceanDNA-b21444.fa --refList OceanDNA-b21444/target_genomes_gtdb.txt --output OceanDNA-b21444/fastani_result_gtdb.tsv --threads 1
[2023-03-16 09:14:05,509] [INFO] Task succeeded: fastANI
[2023-03-16 09:14:05,511] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 09:14:05,511] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018700535.1	s__UM-FILTER-50-9 sp018700535	99.1456	172	179	d__Bacteria;p__Patescibacteria;c__ABY1;o__SG8-24;f__GWF2-40-263;g__UM-FILTER-50-9	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2023-03-16 09:14:05,514] [INFO] GTDB search result was written to OceanDNA-b21444/result_gtdb.tsv
[2023-03-16 09:14:05,516] [INFO] ===== GTDB Search completed =====
[2023-03-16 09:14:05,518] [INFO] DFAST_QC result json was written to OceanDNA-b21444/dqc_result.json
[2023-03-16 09:14:05,518] [INFO] DFAST_QC completed!
[2023-03-16 09:14:05,518] [INFO] Total running time: 0h0m44s