[2023-03-19 03:10:00,741] [INFO] DFAST_QC pipeline started.
[2023-03-19 03:10:00,742] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 03:10:00,742] [INFO] DQC Reference Directory: /var/lib/cwl/stg632ffe05-5f08-4483-84f1-bced5d37bdea/dqc_reference
[2023-03-19 03:10:01,881] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 03:10:01,881] [INFO] Task started: Prodigal
[2023-03-19 03:10:01,881] [INFO] Running command: cat /var/lib/cwl/stg149a22ba-3f9a-4058-b5ea-fd9dc2c8d55f/OceanDNA-b22087.fa | prodigal -d OceanDNA-b22087/cds.fna -a OceanDNA-b22087/protein.faa -g 11 -q > /dev/null
[2023-03-19 03:10:15,579] [INFO] Task succeeded: Prodigal
[2023-03-19 03:10:15,579] [INFO] Task started: HMMsearch
[2023-03-19 03:10:15,579] [INFO] Running command: hmmsearch --tblout OceanDNA-b22087/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg632ffe05-5f08-4483-84f1-bced5d37bdea/dqc_reference/reference_markers.hmm OceanDNA-b22087/protein.faa > /dev/null
[2023-03-19 03:10:15,779] [INFO] Task succeeded: HMMsearch
[2023-03-19 03:10:15,780] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg149a22ba-3f9a-4058-b5ea-fd9dc2c8d55f/OceanDNA-b22087.fa]
[2023-03-19 03:10:15,801] [INFO] Query marker FASTA was written to OceanDNA-b22087/markers.fasta
[2023-03-19 03:10:15,802] [INFO] Task started: Blastn
[2023-03-19 03:10:15,802] [INFO] Running command: blastn -query OceanDNA-b22087/markers.fasta -db /var/lib/cwl/stg632ffe05-5f08-4483-84f1-bced5d37bdea/dqc_reference/reference_markers.fasta -out OceanDNA-b22087/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 03:10:16,498] [INFO] Task succeeded: Blastn
[2023-03-19 03:10:16,499] [INFO] Selected 25 target genomes.
[2023-03-19 03:10:16,499] [INFO] Target genome list was writen to OceanDNA-b22087/target_genomes.txt
[2023-03-19 03:10:16,512] [INFO] Task started: fastANI
[2023-03-19 03:10:16,512] [INFO] Running command: fastANI --query /var/lib/cwl/stg149a22ba-3f9a-4058-b5ea-fd9dc2c8d55f/OceanDNA-b22087.fa --refList OceanDNA-b22087/target_genomes.txt --output OceanDNA-b22087/fastani_result.tsv --threads 1
[2023-03-19 03:10:35,473] [INFO] Task succeeded: fastANI
[2023-03-19 03:10:35,474] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg632ffe05-5f08-4483-84f1-bced5d37bdea/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 03:10:35,474] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg632ffe05-5f08-4483-84f1-bced5d37bdea/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 03:10:35,478] [INFO] Found 3 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 03:10:35,478] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-19 03:10:35,478] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Algisphaera agarilytica	strain=DSM 103725	GCA_014207595.1	1385975	1385975	type	True	77.6568	78	652	95	below_threshold
Phycisphaera mikurensis	strain=DSM 103959	GCA_014207395.1	547188	547188	type	True	75.7341	52	652	95	below_threshold
Phycisphaera mikurensis	strain=NBRC 102666	GCA_000284115.1	547188	547188	type	True	75.7136	53	652	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-19 03:10:35,478] [INFO] DFAST Taxonomy check result was written to OceanDNA-b22087/tc_result.tsv
[2023-03-19 03:10:35,478] [INFO] ===== Taxonomy check completed =====
[2023-03-19 03:10:35,478] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 03:10:35,478] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg632ffe05-5f08-4483-84f1-bced5d37bdea/dqc_reference/checkm_data
[2023-03-19 03:10:35,479] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 03:10:35,508] [INFO] Task started: CheckM
[2023-03-19 03:10:35,508] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b22087/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b22087/checkm_input OceanDNA-b22087/checkm_result
[2023-03-19 03:11:13,310] [INFO] Task succeeded: CheckM
[2023-03-19 03:11:13,310] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.02%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 03:11:13,313] [INFO] ===== Completeness check finished =====
[2023-03-19 03:11:13,313] [INFO] ===== Start GTDB Search =====
[2023-03-19 03:11:13,313] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b22087/markers.fasta)
[2023-03-19 03:11:13,314] [INFO] Task started: Blastn
[2023-03-19 03:11:13,314] [INFO] Running command: blastn -query OceanDNA-b22087/markers.fasta -db /var/lib/cwl/stg632ffe05-5f08-4483-84f1-bced5d37bdea/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b22087/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 03:11:14,471] [INFO] Task succeeded: Blastn
[2023-03-19 03:11:14,472] [INFO] Selected 27 target genomes.
[2023-03-19 03:11:14,472] [INFO] Target genome list was writen to OceanDNA-b22087/target_genomes_gtdb.txt
[2023-03-19 03:11:14,498] [INFO] Task started: fastANI
[2023-03-19 03:11:14,498] [INFO] Running command: fastANI --query /var/lib/cwl/stg149a22ba-3f9a-4058-b5ea-fd9dc2c8d55f/OceanDNA-b22087.fa --refList OceanDNA-b22087/target_genomes_gtdb.txt --output OceanDNA-b22087/fastani_result_gtdb.tsv --threads 1
[2023-03-19 03:11:37,182] [INFO] Task succeeded: fastANI
[2023-03-19 03:11:37,185] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-19 03:11:37,185] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014207595.1	s__Algisphaera agarilytica	77.6955	77	652	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__Phycisphaeraceae;g__Algisphaera	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000284115.1	s__Phycisphaera mikurensis	75.7822	50	652	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__Phycisphaeraceae;g__Phycisphaera	95.0	99.99	99.99	1.00	1.00	2	-
--------------------------------------------------------------------------------
[2023-03-19 03:11:37,185] [INFO] GTDB search result was written to OceanDNA-b22087/result_gtdb.tsv
[2023-03-19 03:11:37,185] [INFO] ===== GTDB Search completed =====
[2023-03-19 03:11:37,186] [INFO] DFAST_QC result json was written to OceanDNA-b22087/dqc_result.json
[2023-03-19 03:11:37,186] [INFO] DFAST_QC completed!
[2023-03-19 03:11:37,186] [INFO] Total running time: 0h1m36s