[2023-03-16 07:18:26,621] [INFO] DFAST_QC pipeline started.
[2023-03-16 07:18:26,621] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 07:18:26,622] [INFO] DQC Reference Directory: /var/lib/cwl/stg03d1dd2d-8bbc-4963-93a9-8f704221e0fd/dqc_reference
[2023-03-16 07:18:27,749] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 07:18:27,749] [INFO] Task started: Prodigal
[2023-03-16 07:18:27,749] [INFO] Running command: cat /var/lib/cwl/stgeb1b375b-aeb7-495f-91d6-479effeebed4/OceanDNA-b22103.fa | prodigal -d OceanDNA-b22103/cds.fna -a OceanDNA-b22103/protein.faa -g 11 -q > /dev/null
[2023-03-16 07:18:47,489] [INFO] Task succeeded: Prodigal
[2023-03-16 07:18:47,490] [INFO] Task started: HMMsearch
[2023-03-16 07:18:47,490] [INFO] Running command: hmmsearch --tblout OceanDNA-b22103/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg03d1dd2d-8bbc-4963-93a9-8f704221e0fd/dqc_reference/reference_markers.hmm OceanDNA-b22103/protein.faa > /dev/null
[2023-03-16 07:18:47,687] [INFO] Task succeeded: HMMsearch
[2023-03-16 07:18:47,688] [INFO] Found 6/6 markers.
[2023-03-16 07:18:47,706] [INFO] Query marker FASTA was written to OceanDNA-b22103/markers.fasta
[2023-03-16 07:18:47,708] [INFO] Task started: Blastn
[2023-03-16 07:18:47,708] [INFO] Running command: blastn -query OceanDNA-b22103/markers.fasta -db /var/lib/cwl/stg03d1dd2d-8bbc-4963-93a9-8f704221e0fd/dqc_reference/reference_markers.fasta -out OceanDNA-b22103/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 07:18:48,261] [INFO] Task succeeded: Blastn
[2023-03-16 07:18:48,262] [INFO] Selected 27 target genomes.
[2023-03-16 07:18:48,263] [INFO] Target genome list was writen to OceanDNA-b22103/target_genomes.txt
[2023-03-16 07:18:48,284] [INFO] Task started: fastANI
[2023-03-16 07:18:48,284] [INFO] Running command: fastANI --query /var/lib/cwl/stgeb1b375b-aeb7-495f-91d6-479effeebed4/OceanDNA-b22103.fa --refList OceanDNA-b22103/target_genomes.txt --output OceanDNA-b22103/fastani_result.tsv --threads 1
[2023-03-16 07:19:05,715] [INFO] Task succeeded: fastANI
[2023-03-16 07:19:05,716] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg03d1dd2d-8bbc-4963-93a9-8f704221e0fd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 07:19:05,716] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg03d1dd2d-8bbc-4963-93a9-8f704221e0fd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 07:19:05,716] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 07:19:05,716] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 07:19:05,716] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 07:19:05,718] [INFO] DFAST Taxonomy check result was written to OceanDNA-b22103/tc_result.tsv
[2023-03-16 07:19:05,718] [INFO] ===== Taxonomy check completed =====
[2023-03-16 07:19:05,718] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 07:19:05,718] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg03d1dd2d-8bbc-4963-93a9-8f704221e0fd/dqc_reference/checkm_data
[2023-03-16 07:19:05,721] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 07:19:05,805] [INFO] Task started: CheckM
[2023-03-16 07:19:05,806] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b22103/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b22103/checkm_input OceanDNA-b22103/checkm_result
[2023-03-16 07:19:58,983] [INFO] Task succeeded: CheckM
[2023-03-16 07:19:58,984] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 07:19:59,013] [INFO] ===== Completeness check finished =====
[2023-03-16 07:19:59,013] [INFO] ===== Start GTDB Search =====
[2023-03-16 07:19:59,014] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b22103/markers.fasta)
[2023-03-16 07:19:59,015] [INFO] Task started: Blastn
[2023-03-16 07:19:59,015] [INFO] Running command: blastn -query OceanDNA-b22103/markers.fasta -db /var/lib/cwl/stg03d1dd2d-8bbc-4963-93a9-8f704221e0fd/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b22103/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 07:19:59,953] [INFO] Task succeeded: Blastn
[2023-03-16 07:19:59,954] [INFO] Selected 13 target genomes.
[2023-03-16 07:19:59,954] [INFO] Target genome list was writen to OceanDNA-b22103/target_genomes_gtdb.txt
[2023-03-16 07:20:00,031] [INFO] Task started: fastANI
[2023-03-16 07:20:00,031] [INFO] Running command: fastANI --query /var/lib/cwl/stgeb1b375b-aeb7-495f-91d6-479effeebed4/OceanDNA-b22103.fa --refList OceanDNA-b22103/target_genomes_gtdb.txt --output OceanDNA-b22103/fastani_result_gtdb.tsv --threads 1
[2023-03-16 07:20:06,795] [INFO] Task succeeded: fastANI
[2023-03-16 07:20:06,803] [INFO] Found 13 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 07:20:06,803] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_009827215.1	s__UBA1924 sp009827215	81.9362	431	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017640485.1	s__UBA1924 sp017640485	80.5223	468	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016764895.1	s__UBA1924 sp016764895	78.8182	342	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002364005.1	s__UBA1924 sp002364005	77.6836	182	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	99.92	99.89	0.95	0.91	4	-
GCA_002337545.1	s__UBA1924 sp002337545	77.0728	167	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	98.57	98.49	0.90	0.89	3	-
GCA_015487555.1	s__UBA1924 sp015487555	77.0133	149	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002401225.1	s__UBA1924 sp002401225	76.8308	105	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017642965.1	s__UBA1924 sp017642965	76.8306	168	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003696675.1	s__UBA6054 sp003696675	76.6751	94	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA6054	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016764015.1	s__UBA1924 sp016764015	76.6343	111	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002429045.1	s__UBA6054 sp002429045	76.3573	98	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA6054	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016763115.1	s__UBA1924 sp016763115	76.0599	70	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002732755.1	s__GCA-2732755 sp002732755	75.7967	50	1017	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__GCA-2732755	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 07:20:06,814] [INFO] GTDB search result was written to OceanDNA-b22103/result_gtdb.tsv
[2023-03-16 07:20:06,815] [INFO] ===== GTDB Search completed =====
[2023-03-16 07:20:06,816] [INFO] DFAST_QC result json was written to OceanDNA-b22103/dqc_result.json
[2023-03-16 07:20:06,816] [INFO] DFAST_QC completed!
[2023-03-16 07:20:06,816] [INFO] Total running time: 0h1m40s