[2023-03-15 23:38:54,771] [INFO] DFAST_QC pipeline started.
[2023-03-15 23:38:54,775] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 23:38:54,775] [INFO] DQC Reference Directory: /var/lib/cwl/stg893f8c11-d7d1-4b9c-ac7d-87d910fa2af4/dqc_reference
[2023-03-15 23:38:55,898] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 23:38:55,898] [INFO] Task started: Prodigal
[2023-03-15 23:38:55,898] [INFO] Running command: cat /var/lib/cwl/stg4f2ce311-16b8-4f35-88a2-bafcd15290ef/OceanDNA-b22162.fa | prodigal -d OceanDNA-b22162/cds.fna -a OceanDNA-b22162/protein.faa -g 11 -q > /dev/null
[2023-03-15 23:39:01,152] [INFO] Task succeeded: Prodigal
[2023-03-15 23:39:01,152] [INFO] Task started: HMMsearch
[2023-03-15 23:39:01,152] [INFO] Running command: hmmsearch --tblout OceanDNA-b22162/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg893f8c11-d7d1-4b9c-ac7d-87d910fa2af4/dqc_reference/reference_markers.hmm OceanDNA-b22162/protein.faa > /dev/null
[2023-03-15 23:39:01,339] [INFO] Task succeeded: HMMsearch
[2023-03-15 23:39:01,340] [INFO] Found 6/6 markers.
[2023-03-15 23:39:01,450] [INFO] Query marker FASTA was written to OceanDNA-b22162/markers.fasta
[2023-03-15 23:39:01,451] [INFO] Task started: Blastn
[2023-03-15 23:39:01,451] [INFO] Running command: blastn -query OceanDNA-b22162/markers.fasta -db /var/lib/cwl/stg893f8c11-d7d1-4b9c-ac7d-87d910fa2af4/dqc_reference/reference_markers.fasta -out OceanDNA-b22162/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 23:39:02,020] [INFO] Task succeeded: Blastn
[2023-03-15 23:39:02,047] [INFO] Selected 13 target genomes.
[2023-03-15 23:39:02,048] [INFO] Target genome list was writen to OceanDNA-b22162/target_genomes.txt
[2023-03-15 23:39:02,055] [INFO] Task started: fastANI
[2023-03-15 23:39:02,055] [INFO] Running command: fastANI --query /var/lib/cwl/stg4f2ce311-16b8-4f35-88a2-bafcd15290ef/OceanDNA-b22162.fa --refList OceanDNA-b22162/target_genomes.txt --output OceanDNA-b22162/fastani_result.tsv --threads 1
[2023-03-15 23:39:09,693] [INFO] Task succeeded: fastANI
[2023-03-15 23:39:09,694] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg893f8c11-d7d1-4b9c-ac7d-87d910fa2af4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 23:39:09,694] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg893f8c11-d7d1-4b9c-ac7d-87d910fa2af4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 23:39:09,694] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 23:39:09,694] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 23:39:09,694] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 23:39:09,704] [INFO] DFAST Taxonomy check result was written to OceanDNA-b22162/tc_result.tsv
[2023-03-15 23:39:09,723] [INFO] ===== Taxonomy check completed =====
[2023-03-15 23:39:09,723] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 23:39:09,723] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg893f8c11-d7d1-4b9c-ac7d-87d910fa2af4/dqc_reference/checkm_data
[2023-03-15 23:39:09,726] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 23:39:09,756] [INFO] Task started: CheckM
[2023-03-15 23:39:09,756] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b22162/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b22162/checkm_input OceanDNA-b22162/checkm_result
[2023-03-15 23:39:29,717] [INFO] Task succeeded: CheckM
[2023-03-15 23:39:29,718] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.92%
Contamintation: 1.91%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 23:39:29,854] [INFO] ===== Completeness check finished =====
[2023-03-15 23:39:29,854] [INFO] ===== Start GTDB Search =====
[2023-03-15 23:39:29,854] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b22162/markers.fasta)
[2023-03-15 23:39:29,855] [INFO] Task started: Blastn
[2023-03-15 23:39:29,855] [INFO] Running command: blastn -query OceanDNA-b22162/markers.fasta -db /var/lib/cwl/stg893f8c11-d7d1-4b9c-ac7d-87d910fa2af4/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b22162/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 23:39:30,791] [INFO] Task succeeded: Blastn
[2023-03-15 23:39:30,822] [INFO] Selected 16 target genomes.
[2023-03-15 23:39:30,823] [INFO] Target genome list was writen to OceanDNA-b22162/target_genomes_gtdb.txt
[2023-03-15 23:39:30,841] [INFO] Task started: fastANI
[2023-03-15 23:39:30,841] [INFO] Running command: fastANI --query /var/lib/cwl/stg4f2ce311-16b8-4f35-88a2-bafcd15290ef/OceanDNA-b22162.fa --refList OceanDNA-b22162/target_genomes_gtdb.txt --output OceanDNA-b22162/fastani_result_gtdb.tsv --threads 1
[2023-03-15 23:39:37,374] [INFO] Task succeeded: fastANI
[2023-03-15 23:39:37,377] [INFO] Found 3 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 23:39:37,377] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_014238125.1	s__GCA-002718515 sp014238125	79.4685	107	241	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014381305.1	s__GCA-002718515 sp014381305	78.2706	98	241	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018656645.1	s__GCA-002718515 sp018656645	78.2425	101	241	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__GCA-002718515	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 23:39:37,384] [INFO] GTDB search result was written to OceanDNA-b22162/result_gtdb.tsv
[2023-03-15 23:39:37,390] [INFO] ===== GTDB Search completed =====
[2023-03-15 23:39:37,401] [INFO] DFAST_QC result json was written to OceanDNA-b22162/dqc_result.json
[2023-03-15 23:39:37,401] [INFO] DFAST_QC completed!
[2023-03-15 23:39:37,401] [INFO] Total running time: 0h0m43s