[2023-03-15 11:10:12,814] [INFO] DFAST_QC pipeline started.
[2023-03-15 11:10:12,824] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 11:10:12,824] [INFO] DQC Reference Directory: /var/lib/cwl/stg9f811207-910f-4616-9ee6-3737a8046eb2/dqc_reference
[2023-03-15 11:10:13,904] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 11:10:13,904] [INFO] Task started: Prodigal
[2023-03-15 11:10:13,904] [INFO] Running command: cat /var/lib/cwl/stg6fd9fd69-15e7-4a7a-8bfe-4b5b1ec08d3f/OceanDNA-b22230.fa | prodigal -d OceanDNA-b22230/cds.fna -a OceanDNA-b22230/protein.faa -g 11 -q > /dev/null
[2023-03-15 11:10:32,219] [INFO] Task succeeded: Prodigal
[2023-03-15 11:10:32,219] [INFO] Task started: HMMsearch
[2023-03-15 11:10:32,219] [INFO] Running command: hmmsearch --tblout OceanDNA-b22230/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9f811207-910f-4616-9ee6-3737a8046eb2/dqc_reference/reference_markers.hmm OceanDNA-b22230/protein.faa > /dev/null
[2023-03-15 11:10:32,424] [INFO] Task succeeded: HMMsearch
[2023-03-15 11:10:32,424] [INFO] Found 6/6 markers.
[2023-03-15 11:10:32,559] [INFO] Query marker FASTA was written to OceanDNA-b22230/markers.fasta
[2023-03-15 11:10:32,560] [INFO] Task started: Blastn
[2023-03-15 11:10:32,560] [INFO] Running command: blastn -query OceanDNA-b22230/markers.fasta -db /var/lib/cwl/stg9f811207-910f-4616-9ee6-3737a8046eb2/dqc_reference/reference_markers.fasta -out OceanDNA-b22230/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 11:10:33,096] [INFO] Task succeeded: Blastn
[2023-03-15 11:10:33,134] [INFO] Selected 23 target genomes.
[2023-03-15 11:10:33,134] [INFO] Target genome list was writen to OceanDNA-b22230/target_genomes.txt
[2023-03-15 11:10:33,146] [INFO] Task started: fastANI
[2023-03-15 11:10:33,146] [INFO] Running command: fastANI --query /var/lib/cwl/stg6fd9fd69-15e7-4a7a-8bfe-4b5b1ec08d3f/OceanDNA-b22230.fa --refList OceanDNA-b22230/target_genomes.txt --output OceanDNA-b22230/fastani_result.tsv --threads 1
[2023-03-15 11:10:49,348] [INFO] Task succeeded: fastANI
[2023-03-15 11:10:49,348] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9f811207-910f-4616-9ee6-3737a8046eb2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 11:10:49,348] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9f811207-910f-4616-9ee6-3737a8046eb2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 11:10:49,348] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 11:10:49,348] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 11:10:49,348] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 11:10:49,359] [INFO] DFAST Taxonomy check result was written to OceanDNA-b22230/tc_result.tsv
[2023-03-15 11:10:49,380] [INFO] ===== Taxonomy check completed =====
[2023-03-15 11:10:49,380] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 11:10:49,380] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9f811207-910f-4616-9ee6-3737a8046eb2/dqc_reference/checkm_data
[2023-03-15 11:10:49,383] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 11:10:49,395] [INFO] Task started: CheckM
[2023-03-15 11:10:49,395] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b22230/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b22230/checkm_input OceanDNA-b22230/checkm_result
[2023-03-15 11:11:38,795] [INFO] Task succeeded: CheckM
[2023-03-15 11:11:38,796] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 11:11:38,802] [INFO] ===== Completeness check finished =====
[2023-03-15 11:11:38,802] [INFO] ===== Start GTDB Search =====
[2023-03-15 11:11:38,803] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b22230/markers.fasta)
[2023-03-15 11:11:38,804] [INFO] Task started: Blastn
[2023-03-15 11:11:38,804] [INFO] Running command: blastn -query OceanDNA-b22230/markers.fasta -db /var/lib/cwl/stg9f811207-910f-4616-9ee6-3737a8046eb2/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b22230/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 11:11:39,588] [INFO] Task succeeded: Blastn
[2023-03-15 11:11:39,600] [INFO] Selected 12 target genomes.
[2023-03-15 11:11:39,600] [INFO] Target genome list was writen to OceanDNA-b22230/target_genomes_gtdb.txt
[2023-03-15 11:11:39,614] [INFO] Task started: fastANI
[2023-03-15 11:11:39,614] [INFO] Running command: fastANI --query /var/lib/cwl/stg6fd9fd69-15e7-4a7a-8bfe-4b5b1ec08d3f/OceanDNA-b22230.fa --refList OceanDNA-b22230/target_genomes_gtdb.txt --output OceanDNA-b22230/fastani_result_gtdb.tsv --threads 1
[2023-03-15 11:11:45,443] [INFO] Task succeeded: fastANI
[2023-03-15 11:11:45,450] [INFO] Found 11 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 11:11:45,450] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_015487555.1	s__UBA1924 sp015487555	77.8156	283	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002364005.1	s__UBA1924 sp002364005	77.5351	271	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	99.92	99.89	0.95	0.91	4	-
GCA_017642965.1	s__UBA1924 sp017642965	77.3064	202	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002337545.1	s__UBA1924 sp002337545	77.1948	221	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	98.57	98.49	0.90	0.89	3	-
GCA_016764015.1	s__UBA1924 sp016764015	77.1663	215	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_009827215.1	s__UBA1924 sp009827215	77.1061	142	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017640485.1	s__UBA1924 sp017640485	76.863	166	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016764895.1	s__UBA1924 sp016764895	76.6971	100	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003696675.1	s__UBA6054 sp003696675	76.6319	81	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA6054	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016763115.1	s__UBA1924 sp016763115	76.0851	99	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA1924	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002429045.1	s__UBA6054 sp002429045	76.0418	91	915	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__UBA1924;g__UBA6054	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 11:11:45,455] [INFO] GTDB search result was written to OceanDNA-b22230/result_gtdb.tsv
[2023-03-15 11:11:45,455] [INFO] ===== GTDB Search completed =====
[2023-03-15 11:11:45,456] [INFO] DFAST_QC result json was written to OceanDNA-b22230/dqc_result.json
[2023-03-15 11:11:45,456] [INFO] DFAST_QC completed!
[2023-03-15 11:11:45,456] [INFO] Total running time: 0h1m33s