[2023-03-16 07:49:28,794] [INFO] DFAST_QC pipeline started.
[2023-03-16 07:49:28,796] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 07:49:28,796] [INFO] DQC Reference Directory: /var/lib/cwl/stgc778212c-dd1d-446f-a08a-b07120ae8df7/dqc_reference
[2023-03-16 07:49:29,912] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 07:49:29,913] [INFO] Task started: Prodigal
[2023-03-16 07:49:29,913] [INFO] Running command: cat /var/lib/cwl/stg93c79c8b-3670-4c7d-b67b-aaeeef99702c/OceanDNA-b22267.fa | prodigal -d OceanDNA-b22267/cds.fna -a OceanDNA-b22267/protein.faa -g 11 -q > /dev/null
[2023-03-16 07:49:35,751] [INFO] Task succeeded: Prodigal
[2023-03-16 07:49:35,751] [INFO] Task started: HMMsearch
[2023-03-16 07:49:35,751] [INFO] Running command: hmmsearch --tblout OceanDNA-b22267/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc778212c-dd1d-446f-a08a-b07120ae8df7/dqc_reference/reference_markers.hmm OceanDNA-b22267/protein.faa > /dev/null
[2023-03-16 07:49:35,900] [INFO] Task succeeded: HMMsearch
[2023-03-16 07:49:35,900] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg93c79c8b-3670-4c7d-b67b-aaeeef99702c/OceanDNA-b22267.fa]
[2023-03-16 07:49:35,932] [INFO] Query marker FASTA was written to OceanDNA-b22267/markers.fasta
[2023-03-16 07:49:35,933] [INFO] Task started: Blastn
[2023-03-16 07:49:35,933] [INFO] Running command: blastn -query OceanDNA-b22267/markers.fasta -db /var/lib/cwl/stgc778212c-dd1d-446f-a08a-b07120ae8df7/dqc_reference/reference_markers.fasta -out OceanDNA-b22267/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 07:49:36,683] [INFO] Task succeeded: Blastn
[2023-03-16 07:49:36,685] [INFO] Selected 12 target genomes.
[2023-03-16 07:49:36,685] [INFO] Target genome list was writen to OceanDNA-b22267/target_genomes.txt
[2023-03-16 07:49:36,692] [INFO] Task started: fastANI
[2023-03-16 07:49:36,692] [INFO] Running command: fastANI --query /var/lib/cwl/stg93c79c8b-3670-4c7d-b67b-aaeeef99702c/OceanDNA-b22267.fa --refList OceanDNA-b22267/target_genomes.txt --output OceanDNA-b22267/fastani_result.tsv --threads 1
[2023-03-16 07:49:47,668] [INFO] Task succeeded: fastANI
[2023-03-16 07:49:47,668] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc778212c-dd1d-446f-a08a-b07120ae8df7/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 07:49:47,669] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc778212c-dd1d-446f-a08a-b07120ae8df7/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 07:49:47,669] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 07:49:47,669] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 07:49:47,669] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 07:49:47,672] [INFO] DFAST Taxonomy check result was written to OceanDNA-b22267/tc_result.tsv
[2023-03-16 07:49:47,673] [INFO] ===== Taxonomy check completed =====
[2023-03-16 07:49:47,673] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 07:49:47,673] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc778212c-dd1d-446f-a08a-b07120ae8df7/dqc_reference/checkm_data
[2023-03-16 07:49:47,677] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 07:49:47,685] [INFO] Task started: CheckM
[2023-03-16 07:49:47,685] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b22267/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b22267/checkm_input OceanDNA-b22267/checkm_result
[2023-03-16 07:50:10,735] [INFO] Task succeeded: CheckM
[2023-03-16 07:50:10,735] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.37%
Contamintation: 1.45%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-16 07:50:10,739] [INFO] ===== Completeness check finished =====
[2023-03-16 07:50:10,739] [INFO] ===== Start GTDB Search =====
[2023-03-16 07:50:10,739] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b22267/markers.fasta)
[2023-03-16 07:50:10,739] [INFO] Task started: Blastn
[2023-03-16 07:50:10,739] [INFO] Running command: blastn -query OceanDNA-b22267/markers.fasta -db /var/lib/cwl/stgc778212c-dd1d-446f-a08a-b07120ae8df7/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b22267/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 07:50:12,320] [INFO] Task succeeded: Blastn
[2023-03-16 07:50:12,324] [INFO] Selected 10 target genomes.
[2023-03-16 07:50:12,325] [INFO] Target genome list was writen to OceanDNA-b22267/target_genomes_gtdb.txt
[2023-03-16 07:50:12,354] [INFO] Task started: fastANI
[2023-03-16 07:50:12,355] [INFO] Running command: fastANI --query /var/lib/cwl/stg93c79c8b-3670-4c7d-b67b-aaeeef99702c/OceanDNA-b22267.fa --refList OceanDNA-b22267/target_genomes_gtdb.txt --output OceanDNA-b22267/fastani_result_gtdb.tsv --threads 1
[2023-03-16 07:50:17,056] [INFO] Task succeeded: fastANI
[2023-03-16 07:50:17,059] [INFO] Found 3 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 07:50:17,059] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002168105.2	s__UBA8653 sp002168105	87.6837	81	91	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__UBA8653	95.0	99.39	99.39	0.92	0.92	2	-
GCA_004213555.1	s__UBA8653 sp002862275	85.984	82	91	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__UBA8653	95.0	98.78	97.23	0.90	0.87	4	-
GCA_002705085.1	s__UBA8653 sp002705085	84.5227	72	91	d__Bacteria;p__Planctomycetota;c__Phycisphaerae;o__Phycisphaerales;f__SM1A02;g__UBA8653	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 07:50:17,062] [INFO] GTDB search result was written to OceanDNA-b22267/result_gtdb.tsv
[2023-03-16 07:50:17,066] [INFO] ===== GTDB Search completed =====
[2023-03-16 07:50:17,070] [INFO] DFAST_QC result json was written to OceanDNA-b22267/dqc_result.json
[2023-03-16 07:50:17,070] [INFO] DFAST_QC completed!
[2023-03-16 07:50:17,070] [INFO] Total running time: 0h0m48s