[2023-03-15 08:55:06,630] [INFO] DFAST_QC pipeline started.
[2023-03-15 08:55:06,630] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 08:55:06,630] [INFO] DQC Reference Directory: /var/lib/cwl/stgc06974d5-c81c-4a3a-9793-4417e3ecb6f7/dqc_reference
[2023-03-15 08:55:08,347] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 08:55:08,347] [INFO] Task started: Prodigal
[2023-03-15 08:55:08,348] [INFO] Running command: cat /var/lib/cwl/stg7294807d-c8be-4a5d-8b46-cb86388747cf/OceanDNA-b22324.fa | prodigal -d OceanDNA-b22324/cds.fna -a OceanDNA-b22324/protein.faa -g 11 -q > /dev/null
[2023-03-15 08:55:50,431] [INFO] Task succeeded: Prodigal
[2023-03-15 08:55:50,432] [INFO] Task started: HMMsearch
[2023-03-15 08:55:50,432] [INFO] Running command: hmmsearch --tblout OceanDNA-b22324/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc06974d5-c81c-4a3a-9793-4417e3ecb6f7/dqc_reference/reference_markers.hmm OceanDNA-b22324/protein.faa > /dev/null
[2023-03-15 08:55:50,717] [INFO] Task succeeded: HMMsearch
[2023-03-15 08:55:50,717] [INFO] Found 6/6 markers.
[2023-03-15 08:55:50,760] [INFO] Query marker FASTA was written to OceanDNA-b22324/markers.fasta
[2023-03-15 08:55:50,760] [INFO] Task started: Blastn
[2023-03-15 08:55:50,760] [INFO] Running command: blastn -query OceanDNA-b22324/markers.fasta -db /var/lib/cwl/stgc06974d5-c81c-4a3a-9793-4417e3ecb6f7/dqc_reference/reference_markers.fasta -out OceanDNA-b22324/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 08:55:51,283] [INFO] Task succeeded: Blastn
[2023-03-15 08:55:51,284] [INFO] Selected 18 target genomes.
[2023-03-15 08:55:51,284] [INFO] Target genome list was writen to OceanDNA-b22324/target_genomes.txt
[2023-03-15 08:55:51,292] [INFO] Task started: fastANI
[2023-03-15 08:55:51,292] [INFO] Running command: fastANI --query /var/lib/cwl/stg7294807d-c8be-4a5d-8b46-cb86388747cf/OceanDNA-b22324.fa --refList OceanDNA-b22324/target_genomes.txt --output OceanDNA-b22324/fastani_result.tsv --threads 1
[2023-03-15 08:56:07,875] [INFO] Task succeeded: fastANI
[2023-03-15 08:56:07,875] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc06974d5-c81c-4a3a-9793-4417e3ecb6f7/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 08:56:07,875] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc06974d5-c81c-4a3a-9793-4417e3ecb6f7/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 08:56:07,876] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 08:56:07,876] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 08:56:07,876] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 08:56:07,876] [INFO] DFAST Taxonomy check result was written to OceanDNA-b22324/tc_result.tsv
[2023-03-15 08:56:07,876] [INFO] ===== Taxonomy check completed =====
[2023-03-15 08:56:07,876] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 08:56:07,876] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc06974d5-c81c-4a3a-9793-4417e3ecb6f7/dqc_reference/checkm_data
[2023-03-15 08:56:07,879] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 08:56:07,888] [INFO] Task started: CheckM
[2023-03-15 08:56:07,888] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b22324/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b22324/checkm_input OceanDNA-b22324/checkm_result
[2023-03-15 08:57:48,549] [INFO] Task succeeded: CheckM
[2023-03-15 08:57:48,550] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 4.17%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 08:57:48,554] [INFO] ===== Completeness check finished =====
[2023-03-15 08:57:48,554] [INFO] ===== Start GTDB Search =====
[2023-03-15 08:57:48,555] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b22324/markers.fasta)
[2023-03-15 08:57:48,555] [INFO] Task started: Blastn
[2023-03-15 08:57:48,555] [INFO] Running command: blastn -query OceanDNA-b22324/markers.fasta -db /var/lib/cwl/stgc06974d5-c81c-4a3a-9793-4417e3ecb6f7/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b22324/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 08:57:49,235] [INFO] Task succeeded: Blastn
[2023-03-15 08:57:49,236] [INFO] Selected 22 target genomes.
[2023-03-15 08:57:49,236] [INFO] Target genome list was writen to OceanDNA-b22324/target_genomes_gtdb.txt
[2023-03-15 08:57:49,421] [INFO] Task started: fastANI
[2023-03-15 08:57:49,422] [INFO] Running command: fastANI --query /var/lib/cwl/stg7294807d-c8be-4a5d-8b46-cb86388747cf/OceanDNA-b22324.fa --refList OceanDNA-b22324/target_genomes_gtdb.txt --output OceanDNA-b22324/fastani_result_gtdb.tsv --threads 1
[2023-03-15 08:58:05,529] [INFO] Task succeeded: fastANI
[2023-03-15 08:58:05,532] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 08:58:05,532] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_003233175.1	s__SZUA-33 sp003233175	75.3991	51	2071	d__Bacteria;p__Planctomycetota;c__Planctomycetia;o__Pirellulales;f__Pirellulaceae;g__SZUA-33	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003389325.1	s__LB-PLM-3 sp003389325	75.1793	58	2071	d__Bacteria;p__Planctomycetota;c__Planctomycetia;o__Pirellulales;f__PALSA-1355;g__LB-PLM-3	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 08:58:05,532] [INFO] GTDB search result was written to OceanDNA-b22324/result_gtdb.tsv
[2023-03-15 08:58:05,532] [INFO] ===== GTDB Search completed =====
[2023-03-15 08:58:05,533] [INFO] DFAST_QC result json was written to OceanDNA-b22324/dqc_result.json
[2023-03-15 08:58:05,533] [INFO] DFAST_QC completed!
[2023-03-15 08:58:05,533] [INFO] Total running time: 0h2m59s