[2023-03-14 10:55:58,925] [INFO] DFAST_QC pipeline started.
[2023-03-14 10:55:58,946] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 10:55:58,946] [INFO] DQC Reference Directory: /var/lib/cwl/stg3c8e2d87-2b4d-4c76-bea8-699b82905c60/dqc_reference
[2023-03-14 10:56:00,749] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 10:56:00,749] [INFO] Task started: Prodigal
[2023-03-14 10:56:00,749] [INFO] Running command: cat /var/lib/cwl/stgbb87dff3-1424-4c78-ab38-a78a6c2b05c5/OceanDNA-b22452.fa | prodigal -d OceanDNA-b22452/cds.fna -a OceanDNA-b22452/protein.faa -g 11 -q > /dev/null
[2023-03-14 10:56:22,116] [INFO] Task succeeded: Prodigal
[2023-03-14 10:56:22,116] [INFO] Task started: HMMsearch
[2023-03-14 10:56:22,116] [INFO] Running command: hmmsearch --tblout OceanDNA-b22452/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3c8e2d87-2b4d-4c76-bea8-699b82905c60/dqc_reference/reference_markers.hmm OceanDNA-b22452/protein.faa > /dev/null
[2023-03-14 10:56:22,439] [INFO] Task succeeded: HMMsearch
[2023-03-14 10:56:22,440] [INFO] Found 6/6 markers.
[2023-03-14 10:56:22,467] [INFO] Query marker FASTA was written to OceanDNA-b22452/markers.fasta
[2023-03-14 10:56:22,467] [INFO] Task started: Blastn
[2023-03-14 10:56:22,467] [INFO] Running command: blastn -query OceanDNA-b22452/markers.fasta -db /var/lib/cwl/stg3c8e2d87-2b4d-4c76-bea8-699b82905c60/dqc_reference/reference_markers.fasta -out OceanDNA-b22452/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 10:56:22,992] [INFO] Task succeeded: Blastn
[2023-03-14 10:56:22,992] [INFO] Selected 13 target genomes.
[2023-03-14 10:56:22,993] [INFO] Target genome list was writen to OceanDNA-b22452/target_genomes.txt
[2023-03-14 10:56:23,001] [INFO] Task started: fastANI
[2023-03-14 10:56:23,001] [INFO] Running command: fastANI --query /var/lib/cwl/stgbb87dff3-1424-4c78-ab38-a78a6c2b05c5/OceanDNA-b22452.fa --refList OceanDNA-b22452/target_genomes.txt --output OceanDNA-b22452/fastani_result.tsv --threads 1
[2023-03-14 10:56:31,547] [INFO] Task succeeded: fastANI
[2023-03-14 10:56:31,547] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3c8e2d87-2b4d-4c76-bea8-699b82905c60/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 10:56:31,548] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3c8e2d87-2b4d-4c76-bea8-699b82905c60/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 10:56:31,548] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 10:56:31,548] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-14 10:56:31,548] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-14 10:56:31,548] [INFO] DFAST Taxonomy check result was written to OceanDNA-b22452/tc_result.tsv
[2023-03-14 10:56:31,548] [INFO] ===== Taxonomy check completed =====
[2023-03-14 10:56:31,548] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 10:56:31,548] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3c8e2d87-2b4d-4c76-bea8-699b82905c60/dqc_reference/checkm_data
[2023-03-14 10:56:31,551] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 10:56:31,557] [INFO] Task started: CheckM
[2023-03-14 10:56:31,557] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b22452/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b22452/checkm_input OceanDNA-b22452/checkm_result
[2023-03-14 10:57:25,955] [INFO] Task succeeded: CheckM
[2023-03-14 10:57:25,955] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 2.08%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-14 10:57:25,958] [INFO] ===== Completeness check finished =====
[2023-03-14 10:57:25,958] [INFO] ===== Start GTDB Search =====
[2023-03-14 10:57:25,958] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b22452/markers.fasta)
[2023-03-14 10:57:25,959] [INFO] Task started: Blastn
[2023-03-14 10:57:25,959] [INFO] Running command: blastn -query OceanDNA-b22452/markers.fasta -db /var/lib/cwl/stg3c8e2d87-2b4d-4c76-bea8-699b82905c60/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b22452/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 10:57:26,672] [INFO] Task succeeded: Blastn
[2023-03-14 10:57:26,673] [INFO] Selected 21 target genomes.
[2023-03-14 10:57:26,673] [INFO] Target genome list was writen to OceanDNA-b22452/target_genomes_gtdb.txt
[2023-03-14 10:57:26,694] [INFO] Task started: fastANI
[2023-03-14 10:57:26,694] [INFO] Running command: fastANI --query /var/lib/cwl/stgbb87dff3-1424-4c78-ab38-a78a6c2b05c5/OceanDNA-b22452.fa --refList OceanDNA-b22452/target_genomes_gtdb.txt --output OceanDNA-b22452/fastani_result_gtdb.tsv --threads 1
[2023-03-14 10:57:39,712] [INFO] Task succeeded: fastANI
[2023-03-14 10:57:39,714] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-14 10:57:39,715] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_011523945.1	s__Mariniblastus sp011523945	94.2101	1143	1199	d__Bacteria;p__Planctomycetota;c__Planctomycetia;o__Pirellulales;f__Pirellulaceae;g__Mariniblastus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_011087765.1	s__Mariniblastus sp011087765	81.6918	801	1199	d__Bacteria;p__Planctomycetota;c__Planctomycetia;o__Pirellulales;f__Pirellulaceae;g__Mariniblastus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-14 10:57:39,715] [INFO] GTDB search result was written to OceanDNA-b22452/result_gtdb.tsv
[2023-03-14 10:57:39,715] [INFO] ===== GTDB Search completed =====
[2023-03-14 10:57:39,715] [INFO] DFAST_QC result json was written to OceanDNA-b22452/dqc_result.json
[2023-03-14 10:57:39,715] [INFO] DFAST_QC completed!
[2023-03-14 10:57:39,715] [INFO] Total running time: 0h1m41s